EFSA Compendium of Botanicals

Draft - work in progress

Anemone chinensis Bunge // Pulsatilla chinensis (Bunge) Regel

Family:: Ranunculaceae
Approval state:: Approved
Number of articles retrieved:: 337
Number of processed references:: 5

Compositions

#1: esculetin (root) #2: saponins #3: pinoresinol #4: β-peltatin #5: 8-Methoxypsoralen #6: pentacyclic triterpene saponins #7: Saponins (pulsatilla saponin A)

Endpoint Studies

Genotoxicity

Composition #1: Yang, Lu., Wang, Liang., Yin, Zhong-qiong., Li, Fu-xing., Jia, Ren-yong., Wang, Hui., Liu, Yu. Content determination of esculetin in modified Radix Pulsatillae granule by RP-HPLC.. zhongshouyi yiyao zazhi, 2014, vol. 33, no. 1, p. 39–43. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

Esculetin (RF-00003667-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

esculetin (root)

Composition #2: Mimaki, Y., Kuroda, M., Asano, T., Sashida, Y. Triterpene saponins and lignans from the roots of Pulsatilla chinensis and their cytotoxic activity against HL-60 cells. JOURNAL OF NATURAL PRODUCTS, 1999, vol. 62, no. 9, p. 1279–1283. DOI: 10.1021/np9901837. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH
Substance:

Saponins (RF-00004333-PAR)
Additional substance description:

hederagenin 3-O-{O-α-Lrhamnopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-R-Larabinopyranoside} ; among others
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

saponins

Composition #3: Mimaki, Y., Kuroda, M., Asano, T., Sashida, Y. Triterpene saponins and lignans from the roots of Pulsatilla chinensis and their cytotoxic activity against HL-60 cells. JOURNAL OF NATURAL PRODUCTS, 1999, vol. 62, no. 9, p. 1279–1283. DOI: 10.1021/np9901837. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH
Substance:

Pinoresinol (RF-00011088-PAR)
Additional substance description:

(+)-pinoresinol
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

pinoresinol

Composition #4: Mimaki, Y., Kuroda, M., Asano, T., Sashida, Y. Triterpene saponins and lignans from the roots of Pulsatilla chinensis and their cytotoxic activity against HL-60 cells. JOURNAL OF NATURAL PRODUCTS, 1999, vol. 62, no. 9, p. 1279–1283. DOI: 10.1021/np9901837. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH
Substance:

beta-peltatin A (RF-00003711-PAR)
Additional substance description:

β-peltatin
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

β-peltatin

Composition #5: Quan, Gui-Hua., Oh, Sei-Ryang., Song, Hyuk-Hwan., Lee, Hyeong-Kyu., Chin, Young-Won. Preparative Isolation of 8-Methoxypsoralen from the Rhizomes of Pulsatilla chinensis using High-speed Counter-current Chromatography. JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, 2011, vol. 54, no. 4, p. 623–627. DOI: 10.3839/jksabc.2011.094. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH
Substance:

Methoxsalen Methoxsalen (RF-00003863-PAR)
Additional substance description:

8-Methoxypsoralen
Analytical method code:

HPLC with standard detection methods (F019A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

8-Methoxypsoralen

Composition #6: Xu, Qiong-ming., Shu, Zhan., Zhu, Wei-feng., Liu, Yan-li., Li, Xiao-ran., Yang, Shi-lin. Lupane-Type Triterpenoidal Saponins from Pulsatilla chinensis and Their Anticomplement Activities through the Classical Pathway. PLANTA MEDICA, 2013, vol. 79, no. 6, p. 506–512. DOI: 10.1055/s-0032-1328323. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH (70%)
Substance:

pentacyclic triterpene saponins (RF-00004334-PAR)
Additional substance description:

e.g. 23-hydroxy-3β-[(O-α-L-rhamnopyranosyl-(1→2)-O-(β-D-glucopyranosyl-( 1→4)-α-L-arabinopyranosyl)]lup-20(29)-en-28-oicacid 28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

pentacyclic triterpene saponins

Composition #7: Liu, Qiang., Chen, Weichang., Jiao, Yang., Hou, Jianquan., Wu, Qingyu., Liu, Yanli., Qi, Xiaofei. Pulsatilla saponin A, an active molecule from Pulsatilla chinensis, induces cancer cell death and inhibits tumor growth in mouse xenograft models. JOURNAL OF SURGICAL RESEARCH, 2014, vol. 188, no. 2, p. 387–395. DOI: 10.1016/j.jss.2014.01.026. [DOI link]

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH (70%)
Substance:

Saponins (RF-00004333-PAR)
Additional substance description:

Pulsatilla saponin A
Analytical method code:

HPLC-DAD (F587A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Saponins (pulsatilla saponin A)