EFSA Compendium of Botanicals

Draft - work in progress

Tanacetum vulgare L.

Family:: Asteraceae
Approval state:: Approved
Number of articles retrieved:: 976
Number of processed references:: 7

Compositions

#1: Polyacetylene in roots #2: alpha-thujone, living plant, GC-FID, essential oil #3: beta-thujone, living plant, GC-FID, essential oil #4: camphore, living plant, GC-FID, essential oil #5: 1,8-cineole in flowers #6: Artemisia ketone in flowers #7: cis-pinocamphone in flowers #8: Camphor in flowers #9: Pinocarvone in flowers #10: trans-thujone in flowers #11: Myrcene in flowers #12: Umbellulone in flowers #13: Verbenone in flowers #14: 1,8-cineole in aerial parts #15: alpha-Thujone in aerial parts #16: beta-Thujone in aerial parts #17: Artemisia ketone in aerial parts #18: Camphor in aerial parts #19: alpha-thujone in essential oil of aerial parts #20: Artemisia ketone in essential oil of aerial parts #21: 1,8-cineole in essential oil of aerial parts #22: beta-thujone in essential oil of aerial parts #23: trans-dihydrocarvone in essential oil of aerial parts #24: Camphor in essential oil of aerial parts #25: Iso-Pinocamphone in essential oil of aerial parts #26: alpha-terthienyl and related thiophenes in aerial parts

Endpoint Studies

#1: hypoactivity, trembling, breathing difficulty, polyuria, asthenia, acute toxicity in high doses in mice

Genotoxicity

Composition #1: Wishart, DS., Jewison, T., Guo, AC., Wilson, M., Knox, C., Liu, Y., Djoumbou, Y., Mandal, R., Aziat, F., Dong, E., Bouatra, S., Sinelnikov, I., Arndt, D., Xia, J., Liu, P., Yallou, F., Bjorndahl, T., Perez-Pineiro, R., Eisner, R., Allen, F., Neveu, V., Greiner, R., Scalbert, A. 1-Acetoxy-4,6-tetradecadiene-8,10,12-triyne (HMDB30881). human metabolome database, 2012 . .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified
Substance:

polyacetylene derivatives polyacetylene derivatives (RF-00004332-PAR)
Additional substance description:

1-Acetoxy-4,6-tetradecadiene-8,10,12-triyne
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Polyacetylene in roots

Composition #2: Chiasson, H., Belanger, A., Bostanian, N., Vincent, C., Poliquin, A. Acaricidal properties of Artemisia absinthium and Tanacetum vulgare (Asteraceae) essential oils obtained by three methods of extraction . journal of economic entomology, 2001, vol. 94, no. 1, p. 167–171. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

Three processes were compared: Microwave assisted process (MAP), distillation in water (DW) and direct steam distillation (DSD)
Substance:

alpha-thujone (RF-00003676-PAR)
Analytical method code:

GC-FID (F040A)
Result unit:

Percent (G138A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.4-1.6
Internal remarks:

alpha-thujone, living plant, GC-FID, essential oil

Composition #3: Chiasson, H., Belanger, A., Bostanian, N., Vincent, C., Poliquin, A. Acaricidal properties of Artemisia absinthium and Tanacetum vulgare (Asteraceae) essential oils obtained by three methods of extraction . journal of economic entomology, 2001, vol. 94, no. 1, p. 167–171. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

Three processes were compared: Microwave assisted process (MAP), distillation in water (DW) and direct steam distillation (DSD)
Substance:

beta-thujone (RF-00003712-PAR)
Analytical method code:

GC-FID (F040A)
Result unit:

Percent (G138A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

87.6-92.2
Internal remarks:

beta-thujone, living plant, GC-FID, essential oil

Composition #4: Chiasson, H., Belanger, A., Bostanian, N., Vincent, C., Poliquin, A. Acaricidal properties of Artemisia absinthium and Tanacetum vulgare (Asteraceae) essential oils obtained by three methods of extraction . journal of economic entomology, 2001, vol. 94, no. 1, p. 167–171. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

Three processes were compared: Microwave assisted process (MAP), distillation in water (DW) and direct steam distillation (DSD)
Substance:

(-)-camphor (RF-00003469-PAR)
Analytical method code:

GC-FID (F040A)
Result unit:

Percent (G138A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1-1.1
Internal remarks:

camphore, living plant, GC-FID, essential oil

Composition #5: Keskitalo, M., Pehu, E., Simon, JE. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes . biochemical systematics and ecology, 2001, vol. 29, no. 3, p. 267–285. DOI: 10.1016/S0305-1978(00)00056-9. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol followed by petroleum ether
Substance:

1,8-cineole (RF-00003470-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.15-47.12
Internal remarks:

1,8-cineole in flowers

Composition #6: Keskitalo, M., Pehu, E., Simon, JE. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes . biochemical systematics and ecology, 2001, vol. 29, no. 3, p. 267–285. DOI: 10.1016/S0305-1978(00)00056-9. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol followed by petroleum ether
Substance:

artemisia ketone (RF-00004157-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.54-81.36
Internal remarks:

Artemisia ketone in flowers

Composition #7: Keskitalo, M., Pehu, E., Simon, JE. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes . biochemical systematics and ecology, 2001, vol. 29, no. 3, p. 267–285. DOI: 10.1016/S0305-1978(00)00056-9. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol followed by petroleum ether
Substance:

iso-pinocamphone (RF-00003875-PAR)
Additional substance description:

cis-pinocamphone
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.03-19.10
Internal remarks:

cis-pinocamphone in flowers

Composition #8: Keskitalo, M., Pehu, E., Simon, JE. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes . biochemical systematics and ecology, 2001, vol. 29, no. 3, p. 267–285. DOI: 10.1016/S0305-1978(00)00056-9. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol followed by petroleum ether
Substance:

Camphor (RF-00003986-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.06-73.02
Internal remarks:

Camphor in flowers

Composition #9: Keskitalo, M., Pehu, E., Simon, JE. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes . biochemical systematics and ecology, 2001, vol. 29, no. 3, p. 267–285. DOI: 10.1016/S0305-1978(00)00056-9. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol followed by petroleum ether
Substance:

pinocarvone (RF-00003547-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.06-4.38
Internal remarks:

Pinocarvone in flowers

Composition #10: Keskitalo, M., Pehu, E., Simon, JE. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes . biochemical systematics and ecology, 2001, vol. 29, no. 3, p. 267–285. DOI: 10.1016/S0305-1978(00)00056-9. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol followed by petroleum ether
Substance:

alpha-thujone (RF-00003676-PAR)
Additional substance description:

trans-thujone
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.04-81.87
Internal remarks:

trans-thujone in flowers

Composition #11: Keskitalo, M., Pehu, E., Simon, JE. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes . biochemical systematics and ecology, 2001, vol. 29, no. 3, p. 267–285. DOI: 10.1016/S0305-1978(00)00056-9. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol followed by petroleum ether
Substance:

myrcene (RF-00003503-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.13-39.39
Internal remarks:

Myrcene in flowers

Composition #12: Keskitalo, M., Pehu, E., Simon, JE. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes . biochemical systematics and ecology, 2001, vol. 29, no. 3, p. 267–285. DOI: 10.1016/S0305-1978(00)00056-9. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol followed by petroleum ether
Substance:

umbellulone (RF-00011282-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.03-12.18
Internal remarks:

Umbellulone in flowers

Composition #13: Keskitalo, M., Pehu, E., Simon, JE. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes . biochemical systematics and ecology, 2001, vol. 29, no. 3, p. 267–285. DOI: 10.1016/S0305-1978(00)00056-9. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol followed by petroleum ether
Substance:

Verbenone (RF-00010114-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.21-3.49
Internal remarks:

Verbenone in flowers

Composition #14: Rohloff, J., Mordal, R., Dragland, S. Chemotypical variation of tansy (Tanacetum vulgare L.) from 40 different locations in Norway . journal of agricultural and food chemistry, 2004, vol. 52, no. 6, p. 1742–1748. DOI: 10.1021/jf0352430. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

1,8-cineole (RF-00003470-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.5-51.4
Internal remarks:

1,8-cineole in aerial parts

Composition #15: Rohloff, J., Mordal, R., Dragland, S. Chemotypical variation of tansy (Tanacetum vulgare L.) from 40 different locations in Norway . journal of agricultural and food chemistry, 2004, vol. 52, no. 6, p. 1742–1748. DOI: 10.1021/jf0352430. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

alpha-thujone (RF-00003676-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.1-73.5
Internal remarks:

alpha-Thujone in aerial parts

Composition #16: Rohloff, J., Mordal, R., Dragland, S. Chemotypical variation of tansy (Tanacetum vulgare L.) from 40 different locations in Norway . journal of agricultural and food chemistry, 2004, vol. 52, no. 6, p. 1742–1748. DOI: 10.1021/jf0352430. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

beta-thujone (RF-00003712-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

5.0-95.7
Internal remarks:

beta-Thujone in aerial parts

Composition #17: Rohloff, J., Mordal, R., Dragland, S. Chemotypical variation of tansy (Tanacetum vulgare L.) from 40 different locations in Norway . journal of agricultural and food chemistry, 2004, vol. 52, no. 6, p. 1742–1748. DOI: 10.1021/jf0352430. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

artemisia ketone (RF-00004157-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

19.8-71.5
Internal remarks:

Artemisia ketone in aerial parts

Composition #18: Rohloff, J., Mordal, R., Dragland, S. Chemotypical variation of tansy (Tanacetum vulgare L.) from 40 different locations in Norway . journal of agricultural and food chemistry, 2004, vol. 52, no. 6, p. 1742–1748. DOI: 10.1021/jf0352430. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

(-)-camphor (RF-00003469-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.5-39.2
Internal remarks:

Camphor in aerial parts

Composition #19: Mockute, D., Judzentiene, A. Composition of the essential oils of Tanacetum vulgare L. growing wild in Vilnius district (Lithuania) . journal of essential oil research, 2004, vol. 16, no. 6, p. 550–553. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

inflorescences and leaves separated and essential oil obtained by hydrodistillation
Substance:

alpha-thujone (RF-00003676-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.8-71.5
Internal remarks:

alpha-thujone in essential oil of aerial parts

Composition #20: Mockute, D., Judzentiene, A. Composition of the essential oils of Tanacetum vulgare L. growing wild in Vilnius district (Lithuania) . journal of essential oil research, 2004, vol. 16, no. 6, p. 550–553. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

inflorescences and leaves separated and essential oil obtained by hydrodistillation
Substance:

artemisia ketone (RF-00004157-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

13.1-30.5
Internal remarks:

Artemisia ketone in essential oil of aerial parts

Composition #21: Mockute, D., Judzentiene, A. Composition of the essential oils of Tanacetum vulgare L. growing wild in Vilnius district (Lithuania) . journal of essential oil research, 2004, vol. 16, no. 6, p. 550–553. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

inflorescences and leaves separated and essential oil obtained by hydrodistillation
Substance:

1,8-cineole (RF-00003470-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

7.0-32.7
Internal remarks:

1,8-cineole in essential oil of aerial parts

Composition #22: Mockute, D., Judzentiene, A. Composition of the essential oils of Tanacetum vulgare L. growing wild in Vilnius district (Lithuania) . journal of essential oil research, 2004, vol. 16, no. 6, p. 550–553. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

inflorescences and leaves separated and essential oil obtained by hydrodistillation
Substance:

beta-thujone (RF-00003712-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=13.4
Internal remarks:

beta-thujone in essential oil of aerial parts

Composition #23: Mockute, D., Judzentiene, A. Composition of the essential oils of Tanacetum vulgare L. growing wild in Vilnius district (Lithuania) . journal of essential oil research, 2004, vol. 16, no. 6, p. 550–553. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

inflorescences and leaves separated and essential oil obtained by hydrodistillation
Substance:

Dihydrocarvone (RF-00011286-PAR)
Additional substance description:

trans-dihydrocarvone
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=1.0
Internal remarks:

trans-dihydrocarvone in essential oil of aerial parts

Composition #24: Mockute, D., Judzentiene, A. Composition of the essential oils of Tanacetum vulgare L. growing wild in Vilnius district (Lithuania) . journal of essential oil research, 2004, vol. 16, no. 6, p. 550–553. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

inflorescences and leaves separated and essential oil obtained by hydrodistillation
Substance:

Camphor (RF-00003986-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=41.4
Internal remarks:

Camphor in essential oil of aerial parts

Composition #25: Mockute, D., Judzentiene, A. Composition of the essential oils of Tanacetum vulgare L. growing wild in Vilnius district (Lithuania) . journal of essential oil research, 2004, vol. 16, no. 6, p. 550–553. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

inflorescences and leaves separated and essential oil obtained by hydrodistillation
Substance:

iso-pinocamphone (RF-00003875-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC-FID
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=5.8
Internal remarks:

Iso-Pinocamphone in essential oil of aerial parts

Composition #26: TOSI, B., BONORA, A., DALLOLIO, G., BRUNI, A. SCREENING FOR TOXIC THIOPHENE COMPOUNDS FROM CRUDE DRUGS OF THE FAMILY COMPOSITAE USED IN NORTHERN ITALY . phytotherapy research, 1991, vol. 5, no. 2, p. 59–62. DOI: 10.1002/ptr.2650050204. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol extraction of flowers and leaves
Substance:

alpha-terthienyl (RF-00004139-PAR)
Additional substance description:

other related/derivative thiophenes were detected in aerial parts
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Analytical method - additional description:

HPTLC-densitometry (data expressed as total thiophene)
Result unit:

Microgram/gram (G049A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

136
Internal remarks:

alpha-terthienyl and related thiophenes in aerial parts

Endpoint study #1: Lahlou, Sanaa., Israili, Zafar H., Lyoussi, Badiaa. Acute and chronic toxicity of a lyophilised aqueous extract of Tanacetum vulgare leaves in rodents . journal of ethnopharmacology, 2008, vol. 117, no. 2, p. 221–227. DOI: 10.1016/j.jep.2008.01.024. [DOI link]

Study relevance:

Human health
Plant part:

Leaves (as part-nature) (A0EKT)
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

water
Type of toxicological test:

acute toxicity (CHD016TT)
Tested organism:

House mouse (as animal) (A0CNT)
Sex:

Male/Female
Route of exposure:

ORAL: GAVAGE (OECD2231PL)
Number of organisms dosed:

10
Endpoint measured:

Dose level (CHD014EP)
Concentration unit:

Gram/kilogram (G015A)
Effect concentration:

9.9
Critical effect:

NEUROTOXICITY (TOX04A)
Target tissue:

Neurologic (TT004A)
Effect description:

hypoactivity, trembling, breathing difficulty, polyuria, asthenia
Effect or parameter linked to the endpoint:

neurology (CHD027BE)
Internal remarks:

hypoactivity, trembling, breathing difficulty, polyuria, asthenia, acute toxicity in high doses in mice