EFSA Compendium of Botanicals

Draft - work in progress

Strychnos ignatii P.J. Bergius

Family:: Loganiaceae
Approval state:: Approved
Number of articles retrieved:: 62
Number of processed references:: 5

Compositions

#1: Brucine in seeds #2: Strychnine in seeds #3: Strychinine in seeds #4: Brucine in seeds #5: strychnine and pseudostrychnine in stem bark #6: brucine and pseudobrucine in stem bark #7: Longicaudatine in root bark #8: Alkaloids in stem wood #9: Alkaloids in roots

Endpoint Studies

Genotoxicity

Composition #1: Biala, R. Gadi., Tits, M., Wauters, J.-N., Angenot, L. A new HPLC method for the assay of alkaloids in Strychnos nux-vomica and Strychnos ignatii . fitoterapia, 1996, vol. 67, no. 2, p. 163–165. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

mixture of Et2O (1.5 ml), ammonia (0.25 ml) and EtOH (0.5 ml)
Substance:

Brucine (RF-00011214-PAR)
Analytical method code:

HPLC-DAD (F587A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.35-0.67
Internal remarks:

Brucine in seeds

Composition #2: Biala, R. Gadi., Tits, M., Wauters, J.-N., Angenot, L. A new HPLC method for the assay of alkaloids in Strychnos nux-vomica and Strychnos ignatii . fitoterapia, 1996, vol. 67, no. 2, p. 163–165. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

mixture of Et2O (1.5 ml), ammonia (0.25 ml) and EtOH (0.5 ml)
Substance:

strychnine (RF-00003591-PAR)
Analytical method code:

HPLC-DAD (F587A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.84-1.77
Internal remarks:

Strychnine in seeds

Composition #3: Dhalwal, Kamlesh., Shinde, Vaibhav M., Namdeo, Ajay G., Mahadlik, Kakasaheb R., Kadam, Shivajirao. S. Development and validation of a TLC-Densitometric method for the simultaneous quantitation of strychnine and brucine from Strychnos spp. and its formulations . journal of chromatographic science, 2007, vol. 45, no. 10, p. 706–709. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

strychnine (RF-00003591-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

HPTLC-densitometric method
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.811
Internal remarks:

Strychinine in seeds

Composition #4: Dhalwal, Kamlesh., Shinde, Vaibhav M., Namdeo, Ajay G., Mahadlik, Kakasaheb R., Kadam, Shivajirao. S. Development and validation of a TLC-Densitometric method for the simultaneous quantitation of strychnine and brucine from Strychnos spp. and its formulations . journal of chromatographic science, 2007, vol. 45, no. 10, p. 706–709. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

Brucine (RF-00011214-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

HPTLC-densitometric method
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.699
Internal remarks:

Brucine in seeds

Composition #5: BISSET, NG., WALKER, MD. ALKALOIDS FROM STEM BARK OF STRYCHNOS-IGNATII. phytochemistry, 1974, vol. 13, no. 2, p. 525–526. DOI: 10.1016/S0031-9422(00)91254-8. [DOI link]

Language:

English (en)
Plant part:

Bark (as part-nature) (A066X)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

CH2Cl2 (following basification 10% NH,OH--20% aq. Na,CO, (1: 1))
Substance:

strychnine (RF-00003591-PAR)
Additional substance description:

strychnine and pseudostrychnine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

GLC and IR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

strychnine and pseudostrychnine in stem bark

Composition #6: BISSET, NG., WALKER, MD. ALKALOIDS FROM STEM BARK OF STRYCHNOS-IGNATII. phytochemistry, 1974, vol. 13, no. 2, p. 525–526. DOI: 10.1016/S0031-9422(00)91254-8. [DOI link]

Language:

English (en)
Plant part:

Bark (as part-nature) (A066X)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

CH2Cl2 (following basification 10% NH,OH--20% aq. Na,CO, (1: 1))
Substance:

Brucine (RF-00011214-PAR)
Additional substance description:

brucine and pseudobrucine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

GLC and IR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

brucine and pseudobrucine in stem bark

Composition #7: MASSIOT, G., ZECHES, M., MIRAND, C., LEMENOLIVIER, L., DELAUDE, C., BASER, KHC., BAVOVADA, R., BISSET, NG., HYLANDS, PJ., STROMBOM, J., VERPOORTE, R. OCCURRENCE OF LONGICAUDATINE, A NEW TYPE OF BIS-INDOLE BASE AND BISNOR-C-ALKALOID-H IN STRYCHNOS SPECIES . journal of organic chemistry, 1983, vol. 48, no. 11, p. 1869–1872. DOI: 10.1021/jo00159a015. [DOI link]

Language:

English (en)
Plant part:

Bark (as part-nature) (A066X)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

longicaudatine
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Longicaudatine in root bark

Composition #8: DATTA, B., BISSET, NG. ALKALOIDS OF STRYCHNOS-IGNATII. planta medica, 1990, vol. 56, no. 1, p. 133–133. DOI: 10.1055/s-2006-960910. [DOI link]

Language:

English (en)
Plant part:

Wood (as part-nature) (A166P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

pseudostrychnine, strychnine N-oxide, normelinonine B, isostrychnine, protostrychnine, 10-hydroxystrychnine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

UV, IR, NMR, and mass spectra
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Alkaloids in stem wood

Composition #9: DATTA, B., BISSET, NG. ALKALOIDS OF STRYCHNOS-IGNATII. planta medica, 1990, vol. 56, no. 1, p. 133–133. DOI: 10.1055/s-2006-960910. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

pseudostrychnine, strychnine N-oxide, normelinonine B, isostrychnine, protostrychnine, 10-hydroxystrychnine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

UV, IR, NMR, and mass spectra
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Alkaloids in roots