EFSA Compendium of Botanicals

Draft - work in progress

Smilax aspera L.

Family:: Smilacaceae
Approval state:: Approved
Number of articles retrieved:: 159
Number of processed references:: 3

Compositions

#1: Melatonin in leaves #2: Steroidal saponins (curillin G, asparagoside E, asparoside A, asparoside B) in roots #3: Furostanol saponins in roots

Endpoint Studies

Genotoxicity

Composition #1: Zohar, Ram., Izhaki, Ido., Koplovich, Avi., Ben-Shlomo, Rachel. Phytomelatonin in the leaves and fruits of wild perennial plants. phytochemistry letters, 2011, vol. 4, no. 3, p. 222–226. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

1 gram wet leaves, crushed and extracted with 10 mL methanol + 0.1% HCl
Substance:

melatonin (RF-00003485-PAR)
Analytical method code:

Enzyme-linked immunosorbent assay (ELISA) (F080A)
Result unit:

Picogram/gram (G080A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

443
Internal remarks:

Melatonin in leaves

Composition #2: Belhouchet, Z., Sautour, M., Miyamoto, T., Lacaille-Dubois, M. A. Steroidal saponins from the roots of Smilax aspera subsp mauritanica. planta medica, 2008, vol. 74, no. 9, p. 1033–1034. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol:water, 7:3
Substance:

steroidal saponins (RF-00010818-PAR)
Additional substance description:

curillin G, asparagoside E, asparoside A, asparoside B
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

HR-ESI-MS, IR Spec, NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Steroidal saponins (curillin G, asparagoside E, asparoside A, asparoside B) in roots

Composition #3: Ivanova, Antoaneta., Mikhova, Bozhanka., Batsalova, Tsvetelina., Dzhambazov, Balik., Kostova, Ivanka. New furostanol saponins from Smilax aspera L. and their in vitro cytotoxicity . fitoterapia, 2011, vol. 82, no. 2, p. 282–287. DOI: 10.1016/j.fitote.2010.10.012. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

steroidal furostan saponins steroidal furostan saponins (RF-00004397-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

Also: TLC, HRESIMS, NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Furostanol saponins in roots