EFSA Compendium of Botanicals

Draft - work in progress

Scopolia carniolica Jacq.

Family:: Solanaceae
Approval state:: Approved
Number of articles retrieved:: 107
Number of processed references:: 4

Compositions

#1: Scopine in underground parts #2: Atropine in underground parts #3: Scopolamine in underground parts #4: Tropine in underground parts #5: Hyoscine N-oxide in plants #6: Hyoscyamine N-oxides in plants #7: Scopolin in roots #8: Scopoletin in roots #9: Calystegines in roots, leaves, and flowers

Endpoint Studies

Genotoxicity

Composition #1: Bendik, I., Bauerova, O., Bauer, S., Mokry, J., Tomko, J. Alkaloids from Scopolia carniolica. chemicke zvesti, 1958, vol. 12, p. 181–4. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ether
Substance:

Scopine (RF-00004717-PAR)
Analytical method code:

Chromatographic tests (F015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Scopine in underground parts

Composition #2: Bendik, I., Bauerova, O., Bauer, S., Mokry, J., Tomko, J. Alkaloids from Scopolia carniolica. chemicke zvesti, 1958, vol. 12, p. 181–4. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ether
Substance:

Atropine (RF-00000246-TOX)
Analytical method code:

Chromatographic tests (F015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Atropine in underground parts

Composition #3: Bendik, I., Bauerova, O., Bauer, S., Mokry, J., Tomko, J. Alkaloids from Scopolia carniolica. chemicke zvesti, 1958, vol. 12, p. 181–4. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ether
Substance:

Scopolamine (RF-00000248-TOX)
Analytical method code:

Chromatographic tests (F015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Scopolamine in underground parts

Composition #4: Bendik, I., Bauerova, O., Bauer, S., Mokry, J., Tomko, J. Alkaloids from Scopolia carniolica. chemicke zvesti, 1958, vol. 12, p. 181–4. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ether
Substance:

tropine (RF-00004093-PAR)
Analytical method code:

Chromatographic tests (F015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Tropine in underground parts

Composition #5: Phillipson, J. David., Handa, S. S. N-oxides of hyoscyamine and hyoscine in the Solanaceae. phytochemistry (elsevier), 1975, vol. 14, no. 4, p. 999–1003. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

roots, stems, and leaves: 5% NH4OH in MeOH
Substance:

Tropane alkaloids (RF-00000245-TOX)
Additional substance description:

hyoscine N-oxide
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC and MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Hyoscine N-oxide in plants

Composition #6: Phillipson, J. David., Handa, S. S. N-oxides of hyoscyamine and hyoscine in the Solanaceae. phytochemistry (elsevier), 1975, vol. 14, no. 4, p. 999–1003. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

roots, stems, and leaves: 5% NH4OH in MeOH
Substance:

Tropane alkaloids (RF-00000245-TOX)
Additional substance description:

two hyoscyamine N-oxides
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC and MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Hyoscyamine N-oxides in plants

Composition #7: Rollinger, JM., Hornick, A., Langer, T., Stuppner, H., Prast, H. Acetylcholinesterase inhibitory activity of scopolin and scopoletin discovered by virtual screening of natural products . journal of medicinal chemistry, 2004, vol. 47, no. 25, p. 6248–6254. DOI: 10.1021/jm049655r. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

Scopolin (RF-00010995-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC, NMR, LC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Scopolin in roots

Composition #8: Rollinger, JM., Hornick, A., Langer, T., Stuppner, H., Prast, H. Acetylcholinesterase inhibitory activity of scopolin and scopoletin discovered by virtual screening of natural products . journal of medicinal chemistry, 2004, vol. 47, no. 25, p. 6248–6254. DOI: 10.1021/jm049655r. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

scopoletin (RF-00004048-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC, NMR, LC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Scopoletin in roots

Composition #9: Drager, B., vanAlmsick, A., Mrachatz, G. Distribution of calystegines in several Solanaceae. planta medica, 1995, vol. 61, no. 6, p. 577–579. DOI: 10.1055/s-2006-959381. [DOI link]

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

50% Methanol/water
Substance:

Calystegines (RF-00010841-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC and GC
Result unit:

Microgram/gram (G049A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

25-220
Internal remarks:

Calystegines in roots, leaves, and flowers