EFSA Compendium of Botanicals

Draft - work in progress

Ranunculus bulbosus L.

Family:: Ranunculaceae
Approval state:: Approved
Number of articles retrieved:: 186
Number of processed references:: 4

Compositions

#1: protoenemonin in stems #2: anemonin #3: protocatechuic acid in aerial parts #4: Hydroxybenzoic acid in aerial parts #5: p-coumaric acid in aerial parts

Endpoint Studies

#1: abortion in horses #2: weight loss, diarrhea, ulcers and erosions in the stomach and large intestine in horses

Genotoxicity

Composition #1: Bonora, A., Dall'Olio, G., Bruni, A. Separation and quantitation of protoanemonin in Ranunculaceae by normal- and reversed-phase HPLC. planta medica, 1985, vol. (5), p. 364–7. .

Language:

English (en)
Plant part:

Stem / stalk (as part-nature) (A067S)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

n-hexane
Substance:

Protoanemonin (RF-00003593-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Microgram/gram (G049A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

7765.6
Internal remarks:

protoenemonin in stems

Composition #2: Shahzad Aslam, M., Choudhary, B.A., Uzair, M., Subhan Ijaz, A. The genus Ranunculus: A phytochemical and ethnopharmacological review. international journal of pharmacy and pharmaceutical sciences, 2012, vol. 4, no. SUPPL. 5, p. 15–22. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Additional extraction description:

unspecified
Substance:

anemonin (RF-00011185-PAR)
Analytical method - additional description:

unspecified
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

anemonin

Composition #3: Vlcek, J., Klejdus, B., Kuban, V. Determination of phenolic substances in plant materials by capillary electrophoresis and liquid chromatography . chemicke listy, 2002, vol. 96, no. 1, p. 39–44. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethyl ether
Substance:

3,4-Dihydroxybenzoic acid (RF-00008853-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

capillary electrophoresis
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.33-1.42
Internal remarks:

protocatechuic acid in aerial parts

Composition #4: Vlcek, J., Klejdus, B., Kuban, V. Determination of phenolic substances in plant materials by capillary electrophoresis and liquid chromatography . chemicke listy, 2002, vol. 96, no. 1, p. 39–44. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethyl ether
Substance:

4-Hydroxybenzoic acid (RF-00008325-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.14
Internal remarks:

Hydroxybenzoic acid in aerial parts

Composition #5: Vlcek, J., Klejdus, B., Kuban, V. Determination of phenolic substances in plant materials by capillary electrophoresis and liquid chromatography . chemicke listy, 2002, vol. 96, no. 1, p. 39–44. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethyl ether
Substance:

p-coumaric acid (RF-00010717-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

capillary electrophoresis
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.19-0.20
Internal remarks:

p-coumaric acid in aerial parts

Endpoint study #1: Swerczek, Thomas W. Abortions in Thoroughbred mares associated with consumption of bulbosus buttercups (Ranunculus bulbosus L) . javma-journal of the american veterinary medical association, 2016, vol. 248, no. 6, p. 669–672. .

Study relevance:

Animal (target species) health
Plant part:

Aerial part of plants (as part-nature) (A166F)
Type of toxicological test:

case report (CHD060TT)
Tested organism:

Horse (as animal) (A0B9Z)
Sex:

Female
Number of organisms dosed:

7
Control groups used:

no
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

REPRODUCTIVE (TOX05A)
Target tissue:

Urogenital (TT006A)
Effect description:

abortion
Effect or parameter linked to the endpoint:

reproduction (CHD014BE)
Internal remarks:

abortion in horses

Endpoint study #2: Swerczek, Thomas W. Abortions in Thoroughbred mares associated with consumption of bulbosus buttercups (Ranunculus bulbosus L) . javma-journal of the american veterinary medical association, 2016, vol. 248, no. 6, p. 669–672. .

Study relevance:

Animal (target species) health
Plant part:

Aerial part of plants (as part-nature) (A166F)
Type of toxicological test:

case report (CHD060TT)
Tested organism:

Horse (as animal) (A0B9Z)
Sex:

Male/Female
Control groups used:

no
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

SYSTEMIC (TOX01A)
Target tissue:

Digestive (TT002A)
Effect description:

weight loss, severe diarrhea, incoordination, recumbency, paralysis, ulcers and erosions in the stomach and large intestine
Effect or parameter linked to the endpoint:

histopathology non neoplastic (CHD025BE)
Internal remarks:

weight loss, diarrhea, ulcers and erosions in the stomach and large intestine in horses