Composition #1: Vieira, Marianne M., Campelo, Yuri D M., Sakai, Vanessa Y., Lima, David F., Carvalho, Paulo S, Jr., Ellena, Javier A., Silva, Paulo R P., Vasconcelos, Luciene C., Godejohann, Markus., Petrilli, Helena M., Constantino, Vera R L., Mascarenhas, Yvonne P., Leite, Jose Roberto De Souza De Almeida., Veras, Leiz M C., Cunha, Vanessa R R., Lima, Filipe C D A., Guimaraes, Maria A. Industrial Scale Isolation, Structural and Spectroscopic Characterization of Epiisopiloturine from Pilocarpus microphyllus Stapf Leaves: A Promising Alkaloid against Schistosomiasis . figshare, 2013 . .
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Plant part:
Leaves (as part-nature) (A0EKT)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
toluene, methylene chloride
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Substance:
imidazole alkaloids (RF-00010804-PAR)
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Additional substance description:
Epiisopiloturine, pilocarpine
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Analytical method code:
High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
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Yes/No Qualitative:
Positive/Present (POS)
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Result type:
Qualitative Value (Binary) (BIN)
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Internal remarks:
epiisopiloturine and pilocarpine in leaves
Composition #2: Lima, David Fernandes., De Lima, Luiza Ianny., Rocha, Jefferson Almeida., De Andrade, Ivanilza Moreira., Grazina, Liliana Goncalves., Villa, Caterina., Meira, Liliana., Veras, Leiz Maria Costa., Azevedo, Iabita Fabiana Sousa., Biase, Adriele Giaretta., Costa, Joana., Oliveira, Maria Beatriz PP., Mafra, Isabel., Roberto De Souza De Almeida Leite, Jose. Pilocarpine (%, w/w) contents in S01, S02, and S03 over one year, measured with HPLC. . figshare, 2017 . DOI: http://dx.doi.org/10.1371/journal.pone.0170281.t002.
[
DOI link]
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Plant part:
Leaves (as part-nature) (A0EKT)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
chloroform
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Substance:
Epiisopiloturine (RF-00013336-PAR)
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Analytical method code:
High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
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Result unit:
Percent (G138A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
0.019-0.383
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Internal remarks:
epiisopiloturine in leaves
Composition #3: Lima, David Fernandes., De Lima, Luiza Ianny., Rocha, Jefferson Almeida., De Andrade, Ivanilza Moreira., Grazina, Liliana Goncalves., Villa, Caterina., Meira, Liliana., Veras, Leiz Maria Costa., Azevedo, Iabita Fabiana Sousa., Biase, Adriele Giaretta., Costa, Joana., Oliveira, Maria Beatriz PP., Mafra, Isabel., Roberto De Souza De Almeida Leite, Jose. Pilocarpine (%, w/w) contents in S01, S02, and S03 over one year, measured with HPLC. . figshare, 2017 . DOI: http://dx.doi.org/10.1371/journal.pone.0170281.t002.
[
DOI link]
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Plant part:
Leaves (as part-nature) (A0EKT)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
chloroform
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Substance:
pilocarpine (RF-00003545-PAR)
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Analytical method code:
High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
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Result unit:
Percent (G138A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
0.219-0.947
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Internal remarks:
pilocarpine in leaves
Composition #4: Sandhu, Sardul Singh., Abreu, Ilka Nacif., Colombo, Carlos Augusto., Mazzafera, Paulo. Pilocarpine content and molecular diversity in Jaborandi. scientia agricola, 2006, vol. 63, no. 5, p. 478–482. .
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Plant part:
Leaves (as part-nature) (A0EKT)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
methanol
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Substance:
pilocarpine (RF-00003545-PAR)
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Analytical method code:
High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
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Result unit:
Microgram/gram (G049A)
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Result expression:
Dry matter (B002A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
16.3-235.9
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Internal remarks:
Pilocarpine in leaves
Composition #5: Abreu, Ilka N., Mazzafera, Paulo., Eberlin, Marcos N., Zullo, Marco Antonio T., Sawaya, Alexandra C. H. F. Characterization of the variation in the imidazole alkaloid profile of Pilocarpus microphyllus in different seasons and parts of the plant by electrospray ionization mass spectrometry fingerprinting and identification of novel alkaloids by tandem mass spectrometry . rapid communications in mass spectrometry, 2007, vol. 21, no. 7, p. 1205–1213. DOI: 10.1002/rcm.2942.
[
DOI link]
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Plant part:
Leaves (as part-nature) (A0EKT)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
10% NH4OH
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Substance:
imidazole alkaloids (RF-00010804-PAR)
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Additional substance description:
pilosinine
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Analytical method code:
HPLC with standard detection methods (F019A)
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Yes/No Qualitative:
Positive/Present (POS)
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Result type:
Qualitative Value (Binary) (BIN)
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Internal remarks:
pilosinine in leaves
Composition #6: Sawaya, Alexandra C. H. F., Abreu, Ilka Nacif., Andreazza, Nathalia Luiza., Eberlin, Marcos N., Mazzafera, Paulo. HPLC-ESI-MS/MS of imidazole alkaloids in Pilocarpus microphyllus. molecules, 2008, vol. 13, no. 7, p. 1518–1529. DOI: 10.3390/molecules13071518.
[
DOI link]
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Plant part:
Leaves (as part-nature) (A0EKT)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
NaOH 10%
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Substance:
imidazole alkaloids (RF-00010804-PAR)
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Additional substance description:
pilosine and derivatives
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Analytical method code:
HPLC with standard detection methods (F019A)
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Yes/No Qualitative:
Positive/Present (POS)
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Result type:
Qualitative Value (Binary) (BIN)
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Internal remarks:
pilosine and derivatives in leaves
Composition #7: Helena Frankland Sawaya, Alexandra Christine., Vaz, Boniek Gontijo., Eberlin, Marcos Nogueira., Mazzafera, Paulo. Screening species of Pilocarpus (Rutaceae) as sources of pilocarpine and other imidazole alkaloids . genetic resources and crop evolution, 2011, vol. 58, no. 3, p. 471–480. DOI: 10.1007/s10722-011-9660-2.
[
DOI link]
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Plant part:
Leaves (as part-nature) (A0EKT)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
10% NH4OH
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Substance:
Alkaloids (RF-00004360-PAR)
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Additional substance description:
Total alkaloids
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Analytical method code:
HPLC with standard detection methods (F019A)
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Analytical method - additional description:
HPLC-ESI-MS-MS
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Result unit:
Percent (G138A)
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Result expression:
Dry matter (B002A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
0.88-1.08
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Internal remarks:
Total alkaloids in leaves
Composition #8: Helena Frankland Sawaya, Alexandra Christine., Vaz, Boniek Gontijo., Eberlin, Marcos Nogueira., Mazzafera, Paulo. Screening species of Pilocarpus (Rutaceae) as sources of pilocarpine and other imidazole alkaloids . genetic resources and crop evolution, 2011, vol. 58, no. 3, p. 471–480. DOI: 10.1007/s10722-011-9660-2.
[
DOI link]
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-
Plant part:
Leaves (as part-nature) (A0EKT)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
10% NH4OH
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Substance:
pilocarpine (RF-00003545-PAR)
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Analytical method code:
HPLC with standard detection methods (F019A)
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Analytical method - additional description:
HPLC-ESI-MS-MS
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Result unit:
Percent (G138A)
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Result expression:
Whole weight (B001A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
<0.03
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Internal remarks:
pilocarpine (fresh) in leaves
Composition #9: Helena Frankland Sawaya, Alexandra Christine., Vaz, Boniek Gontijo., Eberlin, Marcos Nogueira., Mazzafera, Paulo. Screening species of Pilocarpus (Rutaceae) as sources of pilocarpine and other imidazole alkaloids . genetic resources and crop evolution, 2011, vol. 58, no. 3, p. 471–480. DOI: 10.1007/s10722-011-9660-2.
[
DOI link]
-
-
Plant part:
Leaves (as part-nature) (A0EKT)
-
Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
10% NH4OH
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Substance:
pilocarpine (RF-00003545-PAR)
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Substance used for equivalence:
Alkaloids (RF-00004360-PAR)
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Analytical method code:
HPLC with standard detection methods (F019A)
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Analytical method - additional description:
HPLC-ESI-MS-MS
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Result unit:
Percent (G138A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
23.6-34.5
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Internal remarks:
pilocarpine (vs. total alkaloids) in leaves