EFSA Compendium of Botanicals

Draft - work in progress

Ocimum carnosum (Spreng.) Link & Otto ex Benth. // Ocimum selloi Benth.

Family:: Lamiaceae
Approval state:: Approved
Number of articles retrieved:: 88
Number of processed references:: 8

Compositions

#1: estragole #2: eugenol #3: estragole #4: methyleugenol #5: estragole #6: estragol #7: estragol #8: estragole - flower - essentail oil #9: estragole - leaves - essentail oil #10: methyleugenol - flower - essentail oil #11: methyleugenol - leaves - essentail oil

Endpoint Studies

#1: Deaths and toxicity symptoms (e.g. hypoactivity, ataxia and lethargy) in mouse #2: case report on leaves ingestion: Six day-old newborn who presented cessation of breathing during body bath associated with hypersalivation and general hypotonia lasting about 2 minutes. Initial clinical presentation included hyporeactivity, hypotonia, bradycardia and hypothermia. neurotoxicity

Genotoxicity

Composition #1: Padilha de Paula, Josiane., Gomes-Carneiro, Maria Regina., Paumgartten, Francisco J. R. Chemical composition, toxicity and mosquito repellency of Ocimum selloi oil. . journal of ethnopharmacology, 2003, vol. 88, no. 2-3, p. 253–260. DOI: 10.1016/S0378-8741(03)00233-2. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Substance:

Estragole (RF-00003806-PAR)
Analytical method code:

Unspecified (F598A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

55.3
Internal remarks:

estragole

Composition #2: de Paula, Josiane Padilha., Farago, Paulo V., Ribas, Joao Luiz C., Spinardi, Gisele Mara S., Doll, Patricia M., Artoni, Roberto F., Zawadzki, Sonia F. In vivo evaluation of the mutagenic potential of estragole and eugenol chemotypes of Ocimum selloi Benth. Essential oil . latin american journal of pharmacy, 2007, vol. 26, no. 6, p. 846–851. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

Eugenol (RF-00003807-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

45.11
Internal remarks:

eugenol

Composition #3: de Paula, Josiane Padilha., Farago, Paulo V., Ribas, Joao Luiz C., Spinardi, Gisele Mara S., Doll, Patricia M., Artoni, Roberto F., Zawadzki, Sonia F. In vivo evaluation of the mutagenic potential of estragole and eugenol chemotypes of Ocimum selloi Benth. Essential oil . latin american journal of pharmacy, 2007, vol. 26, no. 6, p. 846–851. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

Estragole (RF-00003806-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

46.33
Internal remarks:

estragole

Composition #4: Agostini, Fabiana., Atti dos Santos, Ana Cristina., Rossato, Marcelo., Pansera, Marcia Regina., dos Santos, Paula Luciana., Serafini, Luciana Atti., Molon, Rosangela., Moyna, Patrick. Essential Oil Yield and Composition of Lamiaceae Species Growing in Southern Brazil . brazilian archives of biology and technology, 2009, vol. 52, no. 2, p. 473–478. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

methyleugenol (RF-00003931-PAR)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

35.68
Internal remarks:

methyleugenol

Composition #5: Costa, Larissa C. B., Pinto, Jose E. B. P., Castro, Evaristo M., Alves, Eduardo., Rosal, Louise F., Bertolucci, Suzan K. V., Alves, Pericles B., Evangelino, Tamara S. Yield and Composition of the Essential Oil of Ocimum selloi Benth. Cultivated Under Colored Netting . journal of essential oil research, 2010, vol. 22, no. 1, p. 34–39. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

Estragole (RF-00003806-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

86.1-93.2
Internal remarks:

estragole

Composition #6: Aparecida Salgado de Morais, L., Ming, Lin Chau., Ortiz Mayo Marques, M., Almeida Meireles, M. A., Mathe, A., Barata, AM., Carlen, C., Craker, L. Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction . xxviii international horticultural congress on science and horticulture for people (ihc2010): a new look at medicinal and aromatic plants seminar, 2011, vol. 925, p. 155–158. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation and supercritical fluid extraction
Substance:

Estragole (RF-00003806-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

4.3-38.3
Internal remarks:

estragol

Composition #7: Facanali, Roselaine., Colombo, Carlos A., Teixeira, Joao P. F., Ming, Lin C., Zucchi, Maria I., Marques, Marcia O. M. Genetic and chemical diversity of native populations of Ocimum selloi Benth . industrial crops and products, 2015, vol. 76, p. 249–257. DOI: 10.1016/j.indcrop.2015.06.045. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodystillation
Substance:

Estragole (RF-00003806-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.3-46.3
Internal remarks:

estragol

Composition #8: Martins, ER., Casali, VWD., Barbosa, LCA., Carazza, F. Essential oil in the taxonomy of Ocimum selloi Benth. journal of the brazilian chemical society, 1997, vol. 8, no. 1, p. 29–32. .

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

Estragole (RF-00003806-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

MS, IR and 1H-NMR
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

92.54
Internal remarks:

estragole - flower - essentail oil

Composition #9: Martins, ER., Casali, VWD., Barbosa, LCA., Carazza, F. Essential oil in the taxonomy of Ocimum selloi Benth. journal of the brazilian chemical society, 1997, vol. 8, no. 1, p. 29–32. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

Estragole (RF-00003806-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

MS, IR and 1H-NMR
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

94.95
Internal remarks:

estragole - leaves - essentail oil

Composition #10: Martins, ER., Casali, VWD., Barbosa, LCA., Carazza, F. Essential oil in the taxonomy of Ocimum selloi Benth. journal of the brazilian chemical society, 1997, vol. 8, no. 1, p. 29–32. .

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

methyleugenol (RF-00003931-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

MS, IR and 1H-NMR
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

66.18
Internal remarks:

methyleugenol - flower - essentail oil

Composition #11: Martins, ER., Casali, VWD., Barbosa, LCA., Carazza, F. Essential oil in the taxonomy of Ocimum selloi Benth. journal of the brazilian chemical society, 1997, vol. 8, no. 1, p. 29–32. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

methyleugenol (RF-00003931-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

MS, IR and 1H-NMR
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

65.49
Internal remarks:

methyleugenol - leaves - essentail oil

Endpoint study #1: Padilha de Paula, Josiane., Gomes-Carneiro, Maria Regina., Paumgartten, Francisco J. R. Chemical composition, toxicity and mosquito repellency of Ocimum selloi oil. . journal of ethnopharmacology, 2003, vol. 88, no. 2-3, p. 253–260. DOI: 10.1016/S0378-8741(03)00233-2. [DOI link]

Study relevance:

Human health
Plant part:

Leaves (as part-nature) (A0EKT)
Preparation/Extraction method:

Essential oil (as part-nature) (A166Q)
Additional plant/substance description:

hydrodistillation
Type of toxicological test:

short-term toxicity (CHD017TT)
Tested organism:

House mouse (as animal) (A0CNT)
Strain of test organism:

SwissWebster (CHD006ST)
Sex:

Male/Female
Route of exposure:

ORAL: GAVAGE (OECD2231PL)
Exposure duration:

14.0
Unit of exposure duration:

Day (G134A)
Endpoint measured:

Amount of drug required to induce mortality (CHD076EP)
Concentration unit:

Milligram/kilogram (G061A)
Effect concentration:

1250
Critical effect:

SYSTEMIC (TOX01A)
Target tissue:

neurologic: Central Nervous System (TT035A)
Effect description:

Deaths and symptoms (e.g. hypoactivity, ataxia and lethargy)
Effect or parameter linked to the endpoint:

mortality (CHD010BE)
Internal remarks:

Deaths and toxicity symptoms (e.g. hypoactivity, ataxia and lethargy) in mouse

Endpoint study #2: Vigna1, Andrés., Valdez, Maríanoel., Pascale, Antonio., Moraes4, Mario., Borbonet5, Daniel., Laborde6, Amalia. Intoxicación por anís de campo (Ocimum selloi).: A propósito de un caso. archivos de pediatría del uruguay, 2015, vol. 86, no. 4, p. 300–303. .

Study relevance:

Human health
Plant part:

Leaves (as part-nature) (A0EKT)
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

water - infusion
Type of toxicological test:

case report (CHD060TT)
Tested organism:

Human (as organism) (A056J)
Sex:

Male
Route of exposure:

ORAL: DRINKING WATER (OECD2229PL)
Exposure duration:

1.0
Unit of exposure duration:

Day (G134A)
Number of organisms dosed:

1
Control groups used:

no
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

NEUROTOXICITY (TOX04A)
Target tissue:

Neurologic (TT004A)
Effect description:

Six day-old newborn who presented cessation of breathing during body bath associated with hypersalivation and general hypotonia lasting about 2 minutes. Initial clinical presentation included hyporeactivity, hypotonia, bradycardia and hypothermia
Effect or parameter linked to the endpoint:

neurology (CHD027BE)
Internal remarks:

case report on leaves ingestion: Six day-old newborn who presented cessation of breathing during body bath associated with hypersalivation and general hypotonia lasting about 2 minutes. Initial clinical presentation included hyporeactivity, hypotonia, bradycardia and hypothermia. neurotoxicity