EFSA Compendium of Botanicals

Draft - work in progress

Nigella (as plant) // Nigella sativa L.

Family:: Ranunculaceae
Approval state:: Approved
Number of articles retrieved:: 4059
Number of processed references:: 3

Compositions

#1: Thymoquinone in seeds

Endpoint Studies

#1: acute toxicity - rat

Genotoxicity

#1: intermediate mutagenicity - salmonella

Composition #1: Khalil, W.K.B., Abdel-Gawad, F.K.H., Belattar, N., Senator, A., Abdel-Wahhab, M.A. Protective effects of Nigella sativa extract against chromiumvi-induced genotoxicity in Nile Tilapia (Oreochromis niloticus) and zebrafish (Danio rerio). global veterinaria, 2011, vol. 7, no. 3, p. 283–293. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Substance:

thymoquinone (RF-00004084-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Thymoquinone in seeds

Endpoint study #1: Vahdati-Mashhadian, N., Rakhshandeh, H., Omidi, A. An investigation on LD50 and subacute hepatic toxicity of Nigella sativa seed extracts in mice . pharmazie, 2005, vol. 60, no. 7, p. 544–547. .

Study relevance:

Human health
Plant part:

Seed (as part-nature) (A066P)
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

methanol and chloroform extract
Type of toxicological test:

acute toxicity (CHD016TT)
Tested organism:

Rat (as animal) (A0CNV)
Sex:

Male/Female
Route of exposure:

ORAL: DRINKING WATER (OECD2229PL)
Unit of exposure duration:

single (food/feed) (G203A)
Number of organisms dosed:

8
Control groups used:

yes
Endpoint measured:

Dose level (CHD014EP)
Concentration unit:

Gram/kilogram (G015A)
Effect concentration:

21
Critical effect:

Digestive (TOX18A)
Target tissue:

digestive: Stomach (TT027A)
Effect description:

weight loss
Effect or parameter linked to the endpoint:

body weight (CHD005BE)
Internal remarks:

acute toxicity - rat

Genotox #1: Mahmoud, I., Alkofahi, A., Abdelaziz, A. Mutagenic and toxic activities of several spices and some jordanian medicinal plants. pharmaceutical biology, 1992, vol. 30, no. 2, p. 81–85. DOI: 10.3109/13880209209053961. [DOI link]

Study category:

Mutagenicity
Type of toxicological test:

bacterial gene mutation assay (CHD027TT)
Method type:

in vitro
Tested organism:

Salmonella typhimurium (as organism) (A0CPZ)
Strain of test organism:

TA 102 (CHD025ST)
Is genotoxic:

Positive
Remarks on genotox study:

intermediate mutagenic activity
Internal remarks:

intermediate mutagenicity - salmonella
Internal ID:

eid=2-s2.0-84907105429