EFSA Compendium of Botanicals

Draft - work in progress

Nigella damascena L.

Family:: Ranunculaceae
Approval state:: Approved
Number of articles retrieved:: 319
Number of processed references:: 5

Compositions

#1: Damascenine in seeds #2: Damasterpenes I-IV in seeds #3: Oxazonigelladine (an alkaloid) in seeds #4: Beta-selinene, gamma-selinene and selina-4(14),11-diene in essential oil from seeds #5: Alpha-selinene and epi-alpha-selinene in essential oil from seeds #6: Beta-selinene, gamma-selinene and selina-4(14),11-diene in extract from seeds #7: Alpha-selinene and epi-alpha-selinene in extract from seeds #8: 7-epi-alpha-selinene in essential oil from seeds #9: Damascenine in essential oil from seeds #10: Valencene (naphthalene derivative) in extract of seeds #11: 7-epi-alpha-selinene in extract of seeds #12: Damascenine in extract of seeds #13: Damascenine in essential oil from seeds #14: 7-epi-alpha-selinene in essential oil from seeds #15: 5-Epi-Paradisiol (naphthalene derivative) in extract of seeds

Endpoint Studies

Genotoxicity

Composition #1: Ewins, Arthur J. Constitution and Synthesis of Damascenine, the Alkaloid of Nigella damascena. proc. chem soc., 1912, vol. 28, p. 38. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Light petroleum
Substance:

damascenine (RF-00004249-PAR)
Analytical method code:

Traditional analytical techniques (wet chemical tests) (F003A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.32
Internal remarks:

Damascenine in seeds

Composition #2: Ogawa, Keiko., Nakamura, Seikou., Asada, Yumiko., Yamashita, Masayuki., Matsuda, Hisashi. Oxazonigelladine and dolabellane-type diterpene constituents from Nigella damascena seeds. tetrahedron, 2017, p. Ahead of Print. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

Diterpene esters (RF-00010831-PAR)
Additional substance description:

4 novel dolabellane-type diterpenes, named "Damasterpenes I, II, III, and IV"
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

Also: IR spec, EI Mass Spec, UV spec
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Damasterpenes I-IV in seeds

Composition #3: Ogawa, Keiko., Nakamura, Seikou., Asada, Yumiko., Yamashita, Masayuki., Matsuda, Hisashi. Oxazonigelladine and dolabellane-type diterpene constituents from Nigella damascena seeds. tetrahedron, 2017, p. Ahead of Print. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

Oxazonigelladine, a novel isoxazolidinone derivative
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

Also: IR spec, EI Mass Spec, UV spec
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Oxazonigelladine (an alkaloid) in seeds

Composition #4: Fico, G., Bader, A., Flamini, G., Cioni, PL., Morelli, L. Essential oil of Niagella damascena L. (Ranunculaceae) seeds. journal of essential oil research, 2003, vol. 15, no. 1, p. 57–58. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

Hydrodistillation
Substance:

beta-selinene (RF-00011306-PAR)
Additional substance description:

Related compounds also reported: gamma-selinene (1.7%), and selina-4(14),11-diene (3.2%)
Analytical method code:

GC-MS (Q) (F344A)
Analytical method - additional description:

Additional quantitative analysis by DELSI DI 700
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

4.8
Internal remarks:

Beta-selinene, gamma-selinene and selina-4(14),11-diene in essential oil from seeds

Composition #5: Fico, G., Bader, A., Flamini, G., Cioni, PL., Morelli, L. Essential oil of Niagella damascena L. (Ranunculaceae) seeds. journal of essential oil research, 2003, vol. 15, no. 1, p. 57–58. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

Hydrodistillation
Substance:

alpha-Selinene (RF-00010780-PAR)
Additional substance description:

Related compound also reported: 7 epi-alpha-selinene (2.9%)
Analytical method code:

GC-MS (Q) (F344A)
Analytical method - additional description:

Additional quantitative analysis by DELSI DI 700
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

7.8
Internal remarks:

Alpha-selinene and epi-alpha-selinene in essential oil from seeds

Composition #6: Fico, G., Bader, A., Flamini, G., Cioni, PL., Morelli, L. Essential oil of Niagella damascena L. (Ranunculaceae) seeds. journal of essential oil research, 2003, vol. 15, no. 1, p. 57–58. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform
Substance:

beta-selinene (RF-00011306-PAR)
Additional substance description:

Related compounds also reported: gamma-selinene (1.9%), and selina-4(14),11-diene (2.2%)
Analytical method code:

GC-MS (Q) (F344A)
Analytical method - additional description:

Additional quantitative analysis by DELSI DI 700
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

7.2
Internal remarks:

Beta-selinene, gamma-selinene and selina-4(14),11-diene in extract from seeds

Composition #7: Fico, G., Bader, A., Flamini, G., Cioni, PL., Morelli, L. Essential oil of Niagella damascena L. (Ranunculaceae) seeds. journal of essential oil research, 2003, vol. 15, no. 1, p. 57–58. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform
Substance:

alpha-Selinene (RF-00010780-PAR)
Additional substance description:

Related compound also reported: 7 epi-alpha-selinene (1.0%)
Analytical method code:

GC-MS (Q) (F344A)
Analytical method - additional description:

Additional quantitative analysis by DELSI DI 700
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

6.5
Internal remarks:

Alpha-selinene and epi-alpha-selinene in extract from seeds

Composition #8: Rchid, H., Nmila, R., Bessiere, JM., Sauvaire, Y., Chokairi, M. Volatile components of Nigella damascena L. and Nigella sativa L. seeds. journal of essential oil research, 2004, vol. 16, no. 6, p. 585–587. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

Hydrodistillation
Substance:

alpha-Selinene (RF-00010780-PAR)
Additional substance description:

Related substance was actually measured: 7-epi-alpha-selinene
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC/EIMS
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

3.5
Internal remarks:

7-epi-alpha-selinene in essential oil from seeds

Composition #9: Rchid, H., Nmila, R., Bessiere, JM., Sauvaire, Y., Chokairi, M. Volatile components of Nigella damascena L. and Nigella sativa L. seeds. journal of essential oil research, 2004, vol. 16, no. 6, p. 585–587. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

Hydrodistillation
Substance:

damascenine (RF-00004249-PAR)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

GC/EIMS
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

3.2
Internal remarks:

Damascenine in essential oil from seeds

Composition #10: Sajfrtova, Marie., Sovova, Helena., Karban, Jindrich. Enrichment of Nigella damascena extract with volatile compounds using supercritical fluid extraction . journal of supercritical fluids, 2014, vol. 94, p. 160–164. DOI: 10.1016/j.supflu.2014.07.013. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Hexane
Substance:

Naphthalene derivatives (RF-00010833-PAR)
Additional substance description:

Valencene
Analytical method code:

GC-FID-MS (F607A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.01-0.85
Internal remarks:

Valencene (naphthalene derivative) in extract of seeds

Composition #11: Sajfrtova, Marie., Sovova, Helena., Karban, Jindrich. Enrichment of Nigella damascena extract with volatile compounds using supercritical fluid extraction . journal of supercritical fluids, 2014, vol. 94, p. 160–164. DOI: 10.1016/j.supflu.2014.07.013. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Hexane
Substance:

alpha-Selinene (RF-00010780-PAR)
Additional substance description:

Actual compound measured: 7-epi-alpha-selinene
Analytical method code:

GC-FID-MS (F607A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.05-0.55
Internal remarks:

7-epi-alpha-selinene in extract of seeds

Composition #12: Sajfrtova, Marie., Sovova, Helena., Karban, Jindrich. Enrichment of Nigella damascena extract with volatile compounds using supercritical fluid extraction . journal of supercritical fluids, 2014, vol. 94, p. 160–164. DOI: 10.1016/j.supflu.2014.07.013. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Hexane
Substance:

damascenine (RF-00004249-PAR)
Analytical method code:

GC-FID-MS (F607A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.20-13.21
Internal remarks:

Damascenine in extract of seeds

Composition #13: Sajfrtova, Marie., Sovova, Helena., Karban, Jindrich. Enrichment of Nigella damascena extract with volatile compounds using supercritical fluid extraction . journal of supercritical fluids, 2014, vol. 94, p. 160–164. DOI: 10.1016/j.supflu.2014.07.013. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

damascenine (RF-00004249-PAR)
Analytical method code:

GC-FID-MS (F607A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

4.49
Internal remarks:

Damascenine in essential oil from seeds

Composition #14: Sajfrtova, Marie., Sovova, Helena., Karban, Jindrich. Enrichment of Nigella damascena extract with volatile compounds using supercritical fluid extraction . journal of supercritical fluids, 2014, vol. 94, p. 160–164. DOI: 10.1016/j.supflu.2014.07.013. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

Hydrodistillation
Substance:

alpha-Selinene (RF-00010780-PAR)
Additional substance description:

Actual compound measured: 7-epi-alpha-selinene
Analytical method code:

GC-FID-MS (F607A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.25
Internal remarks:

7-epi-alpha-selinene in essential oil from seeds

Composition #15: Sajfrtova, Marie., Sovova, Helena., Karban, Jindrich. Enrichment of Nigella damascena extract with volatile compounds using supercritical fluid extraction . journal of supercritical fluids, 2014, vol. 94, p. 160–164. DOI: 10.1016/j.supflu.2014.07.013. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Hexane
Substance:

Naphthalene derivatives (RF-00010833-PAR)
Additional substance description:

5-Epi-Paradisiol
Analytical method code:

GC-FID-MS (F607A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.02-0.13
Internal remarks:

5-Epi-Paradisiol (naphthalene derivative) in extract of seeds