EFSA Compendium of Botanicals

Draft - work in progress

Nicotiana tabacum L.

Family:: Solanaceae
Approval state:: Approved
Number of articles retrieved:: 57537
Number of processed references:: 2

Compositions

#1: anthraquinones #2: tannins #3: coumarins #4: alkaloids #5: cardiac glycosides

Endpoint Studies

Genotoxicity

#1: micronucleus test positive in tobacco chewers

Composition #1: da Silva, Fernanda R., Erdtmann, Bernardo., Dalpiaz, Tiago., Nunes, Emilene., Ferraz, Alexandre., Martins, Tales L. C., Dias, Johny F., da Rosa, Darlan P., Porawskie, Marilene., Bona, Silvia., da Silva, Juliana. Genotoxicity of Nicotiana tabacum leaves on Helix aspersa. genetics and molecular biology, 2013, vol. 36, no. 2, p. 269–275. DOI: 10.1590/S1415-47572013005000020. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

anthraquinones (RF-00004343-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

anthraquinones

Composition #2: da Silva, Fernanda R., Erdtmann, Bernardo., Dalpiaz, Tiago., Nunes, Emilene., Ferraz, Alexandre., Martins, Tales L. C., Dias, Johny F., da Rosa, Darlan P., Porawskie, Marilene., Bona, Silvia., da Silva, Juliana. Genotoxicity of Nicotiana tabacum leaves on Helix aspersa. genetics and molecular biology, 2013, vol. 36, no. 2, p. 269–275. DOI: 10.1590/S1415-47572013005000020. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

Tannins (RF-00004337-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

tannins

Composition #3: da Silva, Fernanda R., Erdtmann, Bernardo., Dalpiaz, Tiago., Nunes, Emilene., Ferraz, Alexandre., Martins, Tales L. C., Dias, Johny F., da Rosa, Darlan P., Porawskie, Marilene., Bona, Silvia., da Silva, Juliana. Genotoxicity of Nicotiana tabacum leaves on Helix aspersa. genetics and molecular biology, 2013, vol. 36, no. 2, p. 269–275. DOI: 10.1590/S1415-47572013005000020. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

coumarins (RF-00004351-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

coumarins

Composition #4: da Silva, Fernanda R., Erdtmann, Bernardo., Dalpiaz, Tiago., Nunes, Emilene., Ferraz, Alexandre., Martins, Tales L. C., Dias, Johny F., da Rosa, Darlan P., Porawskie, Marilene., Bona, Silvia., da Silva, Juliana. Genotoxicity of Nicotiana tabacum leaves on Helix aspersa. genetics and molecular biology, 2013, vol. 36, no. 2, p. 269–275. DOI: 10.1590/S1415-47572013005000020. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

Alkaloids (RF-00004360-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

alkaloids

Composition #5: da Silva, Fernanda R., Erdtmann, Bernardo., Dalpiaz, Tiago., Nunes, Emilene., Ferraz, Alexandre., Martins, Tales L. C., Dias, Johny F., da Rosa, Darlan P., Porawskie, Marilene., Bona, Silvia., da Silva, Juliana. Genotoxicity of Nicotiana tabacum leaves on Helix aspersa. genetics and molecular biology, 2013, vol. 36, no. 2, p. 269–275. DOI: 10.1590/S1415-47572013005000020. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

cardiac glycosides (RF-00010823-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

cardiac glycosides

Genotox #1: Rana, S.V.S., Verma, Y., Singh, G.D. Assessment of genotoxicity amongst smokers, alcoholics, and tobacco chewers of North India using micronucleus assay and urinary 8-hydroxyl−2′-deoxyguanosine, as biomarkers. environmental monitoring and assessment, 2017, vol. 189, no. 8 . DOI: 10.1007/s10661-017-6103-3. [DOI link]

Study category:

Genotoxicity
Type of toxicological test:

micronucleus assay (CHD051TT)
Method type:

in vivo
Tested organism:

Human (as organism) (A056J)
Route of exposure:

OTHER (OECD0PL)
Exposure duration for in vivo:

5.0
Unit of exposure duration:

Year (G207A)
Number of organisms dosed:

11
Endpoint measured:

DNA damage and/or repair
Is genotoxic:

Positive
Remarks on genotox study:

micronucleus test positive (0.47%cells) in tobacco chewers, Tobacco chewers consumed 2.0 g of tobacco on an average per day for the last 5 years.
Internal remarks:

micronucleus test positive in tobacco chewers
Internal ID:

2-s2.0-85023747702