EFSA Compendium of Botanicals

Draft - work in progress

Melittis melissophyllum L.

Family:: Lamiaceae
Approval state:: Approved
Number of articles retrieved:: 69
Number of processed references:: 4

Compositions

#1: Camphor #2: coumarin #3: coumarins in dry leaves #4: coumarins in fresh leaves #5: p-coumaric acid in aerial parts #6: o-coumaric acid in aerial parts #7: protocatechuic acid in aerial parts #8: p-hydroxybenzoic acid in aerial parts

Endpoint Studies

Genotoxicity

Composition #1: Skaltsa-Diamantidis, H., Tsitsa-Tzardi, E., Tzakou, O., Argyriadou, N. Analysis of the essential oil of Melittis melissophyllum L. Subsp. albida Guss. journal of essential oil research, 1991, vol. 3, no. 5, p. 367–368. DOI: 10.1080/10412905.1991.9697961. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

(-)-camphor (RF-00003469-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

3.61
Internal remarks:

Camphor

Composition #2: Maggi, Filippo., Conti, Fabio., Cristalli, Gloria., Giuliani, Claudia., Papa, Fabrizio., Sagratini, Gianni., Vittori, Sauro. Chemical Differences in Volatiles between Melittis melissophyllum L. subsp melissophyllum and subsp albida (Guss) P. W. Ball (Lamiaceae) Determined by Solid-Phase Microextraction (SPME) Coupled with GC/FID and GC/MS . chemistry & biodiversity, 2011, vol. 8, no. 2, p. 325–343. DOI: 10.1002/cbdv.201000262. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Additional extraction description:

Headspace Solid-Phase Microextraction (SPME)
Substance:

Coumarin (RF-00000370-TOX)
Analytical method code:

GC-MS (F046A)
Analytical method - additional description:

HS-SPME-GC-MS
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.2-38.5
Internal remarks:

coumarin

Composition #3: Maggi, F., Barboni, L., Caprioli, G., Papa, F., Ricciutelli, M., Sagratini, G., Vittori, S. HPLC quantification of coumarin in bastard balm (Melittis melissophyllum L., Lamiaceae) . fitoterapia, 2011, vol. 82, no. 8, p. 1215–1221. DOI: 10.1016/j.fitote.2011.08.011. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Ethanol/water (80:20 v/v)
Substance:

Coumarin (RF-00000370-TOX)
Analytical method code:

HPLC-DAD (F587A)
Analytical method - additional description:

HPLC - MS
Result unit:

Milligram/kilogram (G061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

3091-11125
Internal remarks:

coumarins in dry leaves

Composition #4: Maggi, F., Barboni, L., Caprioli, G., Papa, F., Ricciutelli, M., Sagratini, G., Vittori, S. HPLC quantification of coumarin in bastard balm (Melittis melissophyllum L., Lamiaceae) . fitoterapia, 2011, vol. 82, no. 8, p. 1215–1221. DOI: 10.1016/j.fitote.2011.08.011. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Ethanol/water (80:20 v/v)
Substance:

Coumarin (RF-00000370-TOX)
Analytical method code:

HPLC-DAD (F587A)
Analytical method - additional description:

HPLC - MS
Result unit:

Milligram/kilogram (G061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

4588-14392
Internal remarks:

coumarins in fresh leaves

Composition #5: Skrzypczak-Pietraszek, Ewa., Pietraszek, Jacek. Chemical profile and seasonal variation of phenolic acid content in bastard balm (Melittis melissophyllum L., Lamiaceae) . journal of pharmaceutical and biomedical analysis, 2012, vol. 66, p. 154–161. DOI: 10.1016/j.jpba.2012.03.037. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

p-coumaric acid (RF-00010717-PAR)
Analytical method code:

HPLC-DAD (F587A)
Analytical method - additional description:

HPLC
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

40.48-121.40
Internal remarks:

p-coumaric acid in aerial parts

Composition #6: Skrzypczak-Pietraszek, Ewa., Pietraszek, Jacek. Chemical profile and seasonal variation of phenolic acid content in bastard balm (Melittis melissophyllum L., Lamiaceae) . journal of pharmaceutical and biomedical analysis, 2012, vol. 66, p. 154–161. DOI: 10.1016/j.jpba.2012.03.037. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

2-Hydroxycinnamic acid (RF-00010695-PAR)
Additional substance description:

o-coumaric acid
Analytical method code:

HPLC-DAD (F587A)
Analytical method - additional description:

HPLC
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.63-2.61
Internal remarks:

o-coumaric acid in aerial parts

Composition #7: Skrzypczak-Pietraszek, Ewa., Pietraszek, Jacek. Chemical profile and seasonal variation of phenolic acid content in bastard balm (Melittis melissophyllum L., Lamiaceae) . journal of pharmaceutical and biomedical analysis, 2012, vol. 66, p. 154–161. DOI: 10.1016/j.jpba.2012.03.037. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

3,4-Dihydroxybenzoic acid (RF-00008853-PAR)
Additional substance description:

syn for protocatechuic acid
Analytical method code:

HPLC-DAD (F587A)
Analytical method - additional description:

HPLC
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.1-8.22
Internal remarks:

protocatechuic acid in aerial parts

Composition #8: Skrzypczak-Pietraszek, Ewa., Pietraszek, Jacek. Chemical profile and seasonal variation of phenolic acid content in bastard balm (Melittis melissophyllum L., Lamiaceae) . journal of pharmaceutical and biomedical analysis, 2012, vol. 66, p. 154–161. DOI: 10.1016/j.jpba.2012.03.037. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

4-Hydroxybenzoic acid (RF-00008325-PAR)
Additional substance description:

syn for p-hydroxybenzoic acids
Analytical method code:

HPLC-DAD (F587A)
Analytical method - additional description:

HPLC
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

30.21-110.91
Internal remarks:

p-hydroxybenzoic acid in aerial parts