EFSA Compendium of Botanicals

Draft - work in progress

Melilot (as plant) // Melilotus officinalis (L.) Pall.

Family:: Fabaceae
Approval state:: Approved
Number of articles retrieved:: 1159
Number of processed references:: 9

Compositions

#1: medicarpin 3-O-β-glucopyranoside (pterocarpans) #2: coumarins in aerial part #3: Saponins in aerial part #4: umbelliferone in aerial part #5: o-coumaric acid in aerial part #6: genistin in aerial part #7: Coumarins in aerial parts #8: dihydrocoumarin #9: coumarin in leaves and stems #10: coumarin in aerial part #11: Coumarins (coumarin, melilotic acid, o-coumaric acid, 3,4-dihydrocoumarin and ethyl melilotate)

Endpoint Studies

Genotoxicity

Composition #1: Yang, J., Wang, L.-L., Zhou, X.-T., Yuan, J.-Z., Zhang, T.-J., Liu, F. Chemical constituents from Melilotus officinalis. chinese traditional and herbal drugs, 2014, vol. 45, no. 5, p. 622–625. DOI: 10.7501/j.issn.0253-2670.2014.05.005. [DOI link]

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

Pterocarpans (RF-00010808-PAR)
Additional substance description:

medicarpin 3-O-β-glucopyranoside
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

medicarpin 3-O-β-glucopyranoside (pterocarpans)

Composition #2: Pascual, ME., Carretero, ME., Slowing, KV., Villar, A. Simplified screening by TLC of plant drugs. pharmaceutical biology, 2002, vol. 40, no. 2, p. 139–143. DOI: 10.1076/phbi.40.2.139.5849. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

hexane, dichloromethane, ethanol
Substance:

coumarins (RF-00004351-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

coumarins in aerial part

Composition #3: Pascual, ME., Carretero, ME., Slowing, KV., Villar, A. Simplified screening by TLC of plant drugs. pharmaceutical biology, 2002, vol. 40, no. 2, p. 139–143. DOI: 10.1076/phbi.40.2.139.5849. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

hexane, dichloromethane, ethanol
Substance:

Saponins (RF-00004333-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Saponins in aerial part

Composition #4: Gird, Cerasela Elena., Dutu, Ligia Elena., Popescu, Maria Lidia., Pavel, Mariana., Sterie, Alina Titina. EXPERIMENTAL RESEARCH REGARDING THE ACTIVE EXTRACTS, POLYPHENOLS-STANDARDIZED. NOTE II. MELILOTI HERBA - PHARMACOGNOSTIC ANALYSIS OF THE RAW MATERIAL . farmacia, 2009, vol. 57, no. 2, p. 184–191. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

umbelliferone (RF-00004096-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

umbelliferone in aerial part

Composition #5: Gird, Cerasela Elena., Dutu, Ligia Elena., Popescu, Maria Lidia., Pavel, Mariana., Sterie, Alina Titina. EXPERIMENTAL RESEARCH REGARDING THE ACTIVE EXTRACTS, POLYPHENOLS-STANDARDIZED. NOTE II. MELILOTI HERBA - PHARMACOGNOSTIC ANALYSIS OF THE RAW MATERIAL . farmacia, 2009, vol. 57, no. 2, p. 184–191. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

p-coumaric acid (RF-00010717-PAR)
Additional substance description:

o-coumaric acid
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

o-coumaric acid in aerial part

Composition #6: Hanganu, Daniela., Vlase, Laurian., Olah, Neli. LC/MS ANALYSIS OF ISOFLAVONES FROM FABACEAE SPECIES EXTRACTS. farmacia, 2010, vol. 58, no. 2, p. 177–183. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol 50%
Substance:

genistin (RF-00003829-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/millilitre (G079A)
Result expression:

Reconstituted product (B007A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

567.2
Internal remarks:

genistin in aerial part

Composition #7: Nair, R. M., Whittall, A., Hughes, S. J., Craig, A. D., Revell, D. K., Miller, S. M., Powell, T., Auricht, G. C. Variation in coumarin content of Melilotus species grown in South Australia . new zealand journal of agricultural research, 2010, vol. 53, no. 3, p. 201–213. DOI: 10.1080/00288233.2010.495743. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water/methanol
Substance:

coumarins (RF-00004351-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.332
Internal remarks:

Coumarins in aerial parts

Composition #8: Kubrak, Tomasz., Dresler, Slawomir., Szymczak, Grazyna., Bogucka-Kocka, Anna. Rapid Determination of Coumarins in Plants by Capillary Electrophoresis. analytical letters, 2015, vol. 48, no. 18, p. 2819–2832. DOI: 10.1080/00032719.2015.1055573. [DOI link]

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

3,4-Dihydrocoumarin (RF-00001799-PAR)
Analytical method code:

Capillary (Zone) Electrophoresis (CE or CZE) (F071A)
Result unit:

Milligram/gram (G060A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.538
Internal remarks:

dihydrocoumarin

Composition #9: Wu, Cai-Xia., Zhao, Guo-Qi., Liu, Da-Lin., Liu, Su-Jiao., Gun, Xiao-Xiao., Tang, Qian. Discovery and Weed Inhibition Effects of Coumarin as the Predominant Allelochemical of Yellow Sweetclover (Melilotus officinalis) . international journal of agriculture and biology, 2016, vol. 18, no. 1, p. 168–175. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water
Substance:

Coumarin (RF-00000370-TOX)
Analytical method code:

LC-MS (F026A)
Analytical method - additional description:

NMR
Result unit:

Microgram/millilitre (G052A)
Result expression:

Reconstituted product (B007A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

46.78
Internal remarks:

coumarin in leaves and stems

Composition #10: BOURGAUD, F., POUTARAUD, A., GUCKERT, A. EXTRACTION OF COUMARINS FROM PLANT-MATERIAL (LEGUMINOSAE). phytochemical analysis, 1994, vol. 5, no. 3, p. 127–132. DOI: 10.1002/pca.2800050308. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water, ethyl acetate, diethylether, chloroform, methanol,
Substance:

Coumarin (RF-00000370-TOX)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

coumarin in aerial part

Composition #11: Ehlers, D., Platte, S., Bork, WR., Gerard, D., Quirin, KW. HPLC analysis of sweat clover extracts. deutsche lebensmittel-rundschau, 1997, vol. 93, no. 3, p. 77–79. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

coumarins (RF-00004351-PAR)
Additional substance description:

coumarin, melilotic acid, o-coumaric acid, 3,4-dihydrocoumarin and ethyl melilotate
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Coumarins (coumarin, melilotic acid, o-coumaric acid, 3,4-dihydrocoumarin and ethyl melilotate)