EFSA Compendium of Botanicals

Draft - work in progress

Lupinus reflexus Rose

Family:: Fabaceae
Approval state:: Approved
Number of articles retrieved:: 5
Number of processed references:: 3

Compositions

#1: Cyanogenic glycosides in seed #2: Sparteine in seeds #3: Lupanine in seeds #4: 3-hydroxylupanine in seeds #5: 13-hydroxylupanine in seeds #6: Isoflavones in roots and leaves

Endpoint Studies

#1: Trypsin inhibition #2: Trypsin inhibition

Genotoxicity

Composition #1: Ruiz-Lopez, M. A., Garcia-Lopez, P. M., Castaneda-Vazquez, H., Zamora, N. J. F., Garzon-De la Mora, P., Banuelos Pineda, J., Burbano, C., Pedrosa, M. M., Cuadrado, C., Muzquiz, M. Chemical composition and antinutrient content of three Lupinus species from Jalisco, Mexico . journal of food composition and analysis, 2000, vol. 13, no. 3, p. 193–199. DOI: 10.1006/jfca.1999.0887. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Additional extraction description:

unspecified
Substance:

Cyanogenic glycosides (RF-00004375-PAR)
Analytical method code:

Unspecified (F598A)
Analytical method - additional description:

The cyanogenic glycosides were determined by the measurement of released HCN from lupin #our
Result unit:

Milligram/100 gram (G057A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<0.01
Internal remarks:

Cyanogenic glycosides in seed

Composition #2: Ruiz, MA., Sotelo, A. Chemical composition, nutritive value, and toxicology evaluation of Mexican wild lupins . journal of agricultural and food chemistry, 2001, vol. 49, no. 11, p. 5336–5339. DOI: 10.1021/jf010247v. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

dichlormethane
Substance:

(-)-Sparteine (RF-00004059-PAR)
Analytical method code:

Unspecified (F598A)
Analytical method - additional description:

identified by comparison with authentic samples
Result unit:

Milligram/gram (G060A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

26.63
Internal remarks:

Sparteine in seeds

Composition #3: Ruiz, MA., Sotelo, A. Chemical composition, nutritive value, and toxicology evaluation of Mexican wild lupins . journal of agricultural and food chemistry, 2001, vol. 49, no. 11, p. 5336–5339. DOI: 10.1021/jf010247v. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

dichlormethane
Substance:

Lupanine (RF-00003910-PAR)
Analytical method code:

Unspecified (F598A)
Analytical method - additional description:

identified by comparison with authentic samples
Result unit:

Milligram/gram (G060A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.91
Internal remarks:

Lupanine in seeds

Composition #4: Ruiz, MA., Sotelo, A. Chemical composition, nutritive value, and toxicology evaluation of Mexican wild lupins . journal of agricultural and food chemistry, 2001, vol. 49, no. 11, p. 5336–5339. DOI: 10.1021/jf010247v. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

dichlormethane
Substance:

3-Hydroxylupanine (RF-00005707-PAR)
Analytical method code:

Unspecified (F598A)
Analytical method - additional description:

identified by comparison with authentic samples
Result unit:

Milligram/gram (G060A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.16
Internal remarks:

3-hydroxylupanine in seeds

Composition #5: Ruiz, MA., Sotelo, A. Chemical composition, nutritive value, and toxicology evaluation of Mexican wild lupins . journal of agricultural and food chemistry, 2001, vol. 49, no. 11, p. 5336–5339. DOI: 10.1021/jf010247v. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

dichlormethane
Substance:

13-Hydroxylupanine (RF-00005706-PAR)
Analytical method code:

Unspecified (F598A)
Analytical method - additional description:

identified by comparison with authentic samples
Result unit:

Milligram/gram (G060A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.08
Internal remarks:

13-hydroxylupanine in seeds

Composition #6: Stobiecki, Maciej., Staszkow, Anna., Piasecka, Anna., Garcia-Lopez, Pedro M., Zamora-Natera, Francisco., Kachlicki, Piotr. LC-MSMS Profiling of Flavonoid Conjugates in Wild Mexican Lupine, Lupinus reflexus . journal of natural products, 2010, vol. 73, no. 7, p. 1254–1260. DOI: 10.1021/np100139d. [DOI link]

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 80% methanol from leaves and roots
Substance:

isoflavones (RF-00004359-PAR)
Additional substance description:

genistein, 2′-hydroxygenistein, biochanin A, luteone, and 2,3-didehydrokievitone
Analytical method code:

HPLC-UV (F021A)
Analytical method - additional description:

LC + UV + MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isoflavones in roots and leaves

Endpoint study #1: Ruiz-Lopez, M. A., Garcia-Lopez, P. M., Castaneda-Vazquez, H., Zamora, N. J. F., Garzon-De la Mora, P., Banuelos Pineda, J., Burbano, C., Pedrosa, M. M., Cuadrado, C., Muzquiz, M. Chemical composition and antinutrient content of three Lupinus species from Jalisco, Mexico . journal of food composition and analysis, 2000, vol. 13, no. 3, p. 193–199. DOI: 10.1006/jfca.1999.0887. [DOI link]

Study relevance:

Human health
Plant part:

Seed (as part-nature) (A066P)
Type of toxicological test:

in vitro (CHD061TT)
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

Digestive (TOX18A)
Target tissue:

digestive: Pancreas (TT024A)
Effect description:

Trypsin inhibition - 1.37 TUI / mg of sample
Effect or parameter linked to the endpoint:

enzyme inhibition (CHD032BE)
Internal remarks:

Trypsin inhibition

Endpoint study #2: Ruiz, MA., Sotelo, A. Chemical composition, nutritive value, and toxicology evaluation of Mexican wild lupins . journal of agricultural and food chemistry, 2001, vol. 49, no. 11, p. 5336–5339. DOI: 10.1021/jf010247v. [DOI link]

Study relevance:

Human health
Plant part:

Seed (as part-nature) (A066P)
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

dichlormethane
Type of toxicological test:

in vitro (CHD061TT)
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

Digestive (TOX18A)
Target tissue:

digestive: Pancreas (TT024A)
Effect description:

Trypsin inhibition - 0.93 TUI / mg of sample
Effect or parameter linked to the endpoint:

enzyme inhibition (CHD032BE)
Internal remarks:

Trypsin inhibition