EFSA Compendium of Botanicals

Draft - work in progress

Lotus corniculatus L.

Family:: Fabaceae
Approval state:: Approved
Number of articles retrieved:: 6107
Number of processed references:: 3

Compositions

#1: Genistein (aerial parts) #2: Genistin (aerial parts) #3: saponin derivative: soyasaponin I, dehydrosoyasaponin I and pharbitoside A (aerial parts) #4: p-coumaric acid (aerial parts) #5: lotaustralin and linamarin (unfolded leaves from shoot apex)

Endpoint Studies

Genotoxicity

Composition #1: Hanganu, Daniela., Vlase, Laurian., Olah, Neli. Phytochemical analysis of isoflavones from some Fabaceae species extracts. notulae botanicae horti agrobotanici cluj-napoca, 2010, vol. 38, no. 1, p. 57–60. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

50% ethanol
Substance:

genistein (RF-00000297-NTR)
Additional substance description:

un-hydrolysed extract
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Microgram/millilitre (G052A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.26
Internal remarks:

Genistein (aerial parts)

Composition #2: Hanganu, Daniela., Vlase, Laurian., Olah, Neli. Phytochemical analysis of isoflavones from some Fabaceae species extracts. notulae botanicae horti agrobotanici cluj-napoca, 2010, vol. 38, no. 1, p. 57–60. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

50% ethanol
Substance:

genistin (RF-00003829-PAR)
Additional substance description:

un-hydrolysed extract
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Microgram/millilitre (G052A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.75
Internal remarks:

Genistin (aerial parts)

Composition #3: Abderrahmane, M., Bouheroum, M., Malafronte, N., D'ambola, M., Meriem, A., Severino, L. Phytochemical investigation and citotoxic activity of lotus corniculatus. pharmacologyonline, 2014, vol. 3, p. 222–225. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70% methanol, n-BuOH and chloroform
Substance:

Saponins (RF-00004333-PAR)
Additional substance description:

saponin derivative: soyasaponin I, dehydrosoyasaponin I and pharbitoside A
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Column chromatography
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

saponin derivative: soyasaponin I, dehydrosoyasaponin I and pharbitoside A (aerial parts)

Composition #4: Abderrahmane, M., Bouheroum, M., Malafronte, N., D'ambola, M., Meriem, A., Severino, L. Phytochemical investigation and citotoxic activity of lotus corniculatus. pharmacologyonline, 2014, vol. 3, p. 222–225. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70% methanol, n-BuOH and chloroform
Substance:

p-coumaric acid (RF-00010717-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Column chromatography-HPLC
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

p-coumaric acid (aerial parts)

Composition #5: Bjarnholt, Nanna., Rook, Fred., Motawia, Mohammed Saddik., Cornett, Claus., Jorgensen, Charlotte., Olsen, Carl Erik., Jaroszewski, Jerzy W., Bak, Soren., Moller, Birger Lindberg. Diversification of an ancient theme: Hydroxynitrile glucosides. phytochemistry, 2008, vol. 69, no. 7, p. 1507–1516. DOI: 10.1016/j.phytochem.2008.01.022. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

80% (v/v) MeOH
Substance:

Cyanogenic glycosides (RF-00004375-PAR)
Additional substance description:

lotaustralin and linamarin
Analytical method code:

LC-MS (F026A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lotaustralin and linamarin (unfolded leaves from shoot apex)