EFSA Compendium of Botanicals

Draft - work in progress

Lobelia inflata L.

Family:: Campanulaceae
Approval state:: Approved
Number of articles retrieved:: 308
Number of processed references:: 4

Compositions

#1: lobelin - alkaloid #2: lobeline - whole plant #3: alkaloid - root #4: lobeline - root #5: alkaloid - whole plant #6: (−)-8-ethyl-10-phenylnorlobelionol, (−)-8-ethyl-10-phenyl-lobelionol, norlobeline, lobelidine, lobelanine norlobelanine, lobelanidine - whole plant #7: piperidine alkaloids: 52 lobeline derivatives in seedlings/ shoots

Endpoint Studies

#1: acro-narcotic poison - lobelia powder in rabbit and dog (vomitting and sleeping)

Genotoxicity

Composition #1: da Costa E Silva, Liana Dantas., Rodrigues, Laise Carla Lima Verde., Dos Santos, Viviane Ramos., da Costa Allgayer, Mariangela., de Barros Falcao Ferraz, Alexandre., Rolla, Helena Campos., Pereira, Patricia., Picada, Jaqueline Nascimento. Evaluation of mutagenic and genotoxic activities of lobeline and its modulation on genomic instability induced by ethanol. . life sciences, 2014, vol. 103, no. 2, p. 73–8. DOI: 10.1016/j.lfs.2014.03.034. [DOI link]

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Substance:

lobeline (RF-00003905-PAR)
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lobelin - alkaloid

Composition #2: Banyai, Peter., Vojnich, Viktor Jozsef., Mathe, Akos., Kursinszki, Laszlo., Szoke, Eva. Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L. . in vitro cellular & developmental biology-plant, 2014, vol. 50, no. 6, p. 760–765. DOI: 10.1007/s11627-014-9639-8. [DOI link]

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

0.5000 g dried and powdered extracted with HCl–methanol
Substance:

lobeline (RF-00003905-PAR)
Analytical method code:

Mass Spectroscopy and hyphenated methods without chromatography (F063A)
Result unit:

Microgram (G283A)
Result expression:

Whole weight (B001A)
Result type:

Non Quantified Value ( less than LOQ) (LOQ)
Value (concentration, content):

21.48-25.04
Internal remarks:

lobeline - whole plant

Composition #3: Banyai, Peter., Vojnich, Viktor Jozsef., Mathe, Akos., Kursinszki, Laszlo., Szoke, Eva. Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L. . in vitro cellular & developmental biology-plant, 2014, vol. 50, no. 6, p. 760–765. DOI: 10.1007/s11627-014-9639-8. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

0.5000 g dried and powdered extracted with HCl–methanol
Substance:

Piperidine alkaloids (RF-00004320-PAR)
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Result unit:

Milligram (G155A)
Result expression:

Whole weight (B001A)
Result type:

Non Quantified Value ( less than LOQ) (LOQ)
Value (concentration, content):

0.29-0.31
Internal remarks:

alkaloid - root

Composition #4: Banyai, Peter., Vojnich, Viktor Jozsef., Mathe, Akos., Kursinszki, Laszlo., Szoke, Eva. Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L. . in vitro cellular & developmental biology-plant, 2014, vol. 50, no. 6, p. 760–765. DOI: 10.1007/s11627-014-9639-8. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

0.5000 g dried and powdered extracted with HCl–methanol
Substance:

lobeline (RF-00003905-PAR)
Analytical method code:

Mass Spectroscopy and hyphenated methods without chromatography (F063A)
Result unit:

Milligram (G155A)
Result expression:

Whole weight (B001A)
Result type:

Non Quantified Value ( less than LOQ) (LOQ)
Value (concentration, content):

5.08-8.88
Internal remarks:

lobeline - root

Composition #5: Banyai, Peter., Vojnich, Viktor Jozsef., Mathe, Akos., Kursinszki, Laszlo., Szoke, Eva. Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L. . in vitro cellular & developmental biology-plant, 2014, vol. 50, no. 6, p. 760–765. DOI: 10.1007/s11627-014-9639-8. [DOI link]

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

0.5000 g dried and powdered extracted with HCl–methanol
Substance:

Piperidine alkaloids (RF-00004320-PAR)
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Result unit:

Milligram (G155A)
Result expression:

Whole weight (B001A)
Result type:

Non Quantified Value ( less than LOQ) (LOQ)
Value (concentration, content):

0.62-0.66
Internal remarks:

alkaloid - whole plant

Composition #6: Banyai, Peter., Vojnich, Viktor Jozsef., Mathe, Akos., Kursinszki, Laszlo., Szoke, Eva. Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L. . in vitro cellular & developmental biology-plant, 2014, vol. 50, no. 6, p. 760–765. DOI: 10.1007/s11627-014-9639-8. [DOI link]

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

0.5000 g dried and powdered extracted with HCl–methanol
Substance:

lobeline (RF-00003905-PAR)
Additional substance description:

(−)-8-ethyl-10-phenylnorlobelionol, (−)-8-ethyl-10-phenyl-lobelionol, norlobeline, lobelidine, lobelanine norlobelanine, lobelanidine
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Result unit:

Microgram (G283A)
Result expression:

Whole weight (B001A)
Result type:

Non Quantified Value ( less than LOQ) (LOQ)
Value (concentration, content):

21.48-25.04
Internal remarks:

(−)-8-ethyl-10-phenylnorlobelionol, (−)-8-ethyl-10-phenyl-lobelionol, norlobeline, lobelidine, lobelanine norlobelanine, lobelanidine - whole plant

Composition #7: Kursinszki, Laszlo., Szoke, Eva. HPLC-ESI-MS/MS of brain neurotransmitter modulator lobeline and related piperidine alkaloids in Lobelia inflata L . journal of mass spectrometry, 2015, vol. 50, no. 5, p. 727–733. DOI: 10.1002/jms.3581. [DOI link]

Language:

English (en)
Plant part:

Shoot (as part-nature) (A067T)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

Piperidine alkaloids (RF-00004320-PAR)
Additional substance description:

52 lobeline derivatives: lobeline, OH-lobeline, diethyllobelidione, phenyllobelinol, lobelone and derivatives, lobelidine, lobelanine, norbeline, etc.
Analytical method code:

HPLC-MS/MS (F698A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

piperidine alkaloids: 52 lobeline derivatives in seedlings/ shoots

Endpoint study #1: Johnson, H. Case of suspected poisoning by lobelia inflata: With the appearances after death. british medical journal, 1858, vol. s4-1, no. 77, p. 493–495. DOI: 10.1136/bmj.s4-1.77.493. [DOI link]

Study relevance:

Animal (target species) health
Plant part:

Live plants (as part-nature) (A0BA0)
Preparation/Extraction method:

Drying (dehydration) (A07KG)
Additional plant/substance description:

lobelia powder
Type of toxicological test:

case report (CHD060TT)
Tested organism:

Rabbit (as animal) (A057T)
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

SYSTEMIC (TOX01A)
Target tissue:

neurologic: Brain (TT034A)
Effect description:

lobelia inflata is a powerful acro-narcotic poison; acts especially upon the brain and the stomach
Effect or parameter linked to the endpoint:

mortality (CHD010BE)
Internal remarks:

acro-narcotic poison - lobelia powder in rabbit and dog (vomitting and sleeping)