EFSA Compendium of Botanicals

Draft - work in progress

Leonurus sibiricus L.

Family:: Lamiaceae
Approval state:: Approved
Number of articles retrieved:: 300
Number of processed references:: 9

Compositions

#1: Tannins of pyrocatechol group - content is 9.43% in blossoms, 9.91/% in leaves, and 5.24% in stems #2: medioresinol #3: syringaresinol #4: pinoresinol O- Beta -D-glucopyranoside in terrestrial parts #5: isoquercitin in terrestrial parts #6: 4-hydroxybenzoic acid in aerial parts #7: isoquercitrin in aerial parts #8: sibiricinones A-E and 15-epi-sibiricinones D and E in aerial parts #9: labdane diterpens: bis-spirolabdane-type diterpenoids (leosibirinone A, 3R-acetoxyleoheteronone C, leosibirinone B, 3R-hydroxyleoheteronone A, 3R-acetoxyleoheteronone E, and 3R-acetoxy-15-epileoheteronone E) in aerial parts #10: phenylpropanoids in aerial parts #11: p-Coumaric acid in roots #12: leojaponin in aerial parts #13: Leonurine in aerial parts

Endpoint Studies

Genotoxicity

Composition #1: Zhelnov, I. I., Grazhdan, A. K. Active substances in Leonurus sibericus. Tannins. sb. tr. posvyashch. xx-letiyu farmatsevt. fak., tomskii med. inst., tomsk, 1962, p. 125–7. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Substance:

Tannins (RF-00004337-PAR)
Additional substance description:

pyrocatechol group of tannins
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Tannins of pyrocatechol group - content is 9.43% in blossoms, 9.91/% in leaves, and 5.24% in stems

Composition #2: Liu, Juan., Zhou, Qin-mei., Peng, Cheng., Liu, Lu-si., Xiong, Liang., Liu, Zhao-hua. Chemical constituents and their anti-platelet aggregation activities of Leonurus sibiricus L.. zhongchengyao, 2015, vol. 37, no. 11, p. 2439–2442. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Substance:

medioresinol (RF-00011065-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

medioresinol

Composition #3: Liu, Juan., Zhou, Qin-mei., Peng, Cheng., Liu, Lu-si., Xiong, Liang., Liu, Zhao-hua. Chemical constituents and their anti-platelet aggregation activities of Leonurus sibiricus L.. zhongchengyao, 2015, vol. 37, no. 11, p. 2439–2442. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Substance:

syringaresinol (RF-00011386-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

syringaresinol

Composition #4: Hayashi, Katsuhiko., Ikoma, Rie., Deyama, Takeshi. Phenolic compounds and iridoids from Leonurus sibiricus. natural medicines, 2001, vol. 55, no. 5, p. 276–276. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol / ether / ethyl acetate / n-BuOH (terrestrial parts)
Substance:

Pinoresinol (RF-00011088-PAR)
Additional substance description:

pinoresinol O- Beta -D-glucopyranoside
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

pinoresinol O- Beta -D-glucopyranoside in terrestrial parts

Composition #5: Hayashi, Katsuhiko., Ikoma, Rie., Deyama, Takeshi. Phenolic compounds and iridoids from Leonurus sibiricus. natural medicines, 2001, vol. 55, no. 5, p. 276–276. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol / ether / ethyl acetate / n-BuOH (terrestrial parts)
Substance:

Isoquercitrin (RF-00010712-PAR)
Additional substance description:

pinoresinol O- Beta -D-glucopyranoside
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

isoquercitin in terrestrial parts

Composition #6: Pan, Sheng-Ming., Ding, Hsiou-Yu., Chang, Wen-Liang., Lin, Hang-Ching. Phenols from the aerial parts of Leonurus sibiricus. chinese pharmaceutical journal (taipei), 2006, vol. 58, no. 1, p. 35–40. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

4-Hydroxybenzoic acid (RF-00008325-PAR)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

4-hydroxybenzoic acid in aerial parts

Composition #7: Pan, Sheng-Ming., Ding, Hsiou-Yu., Chang, Wen-Liang., Lin, Hang-Ching. Phenols from the aerial parts of Leonurus sibiricus. chinese pharmaceutical journal (taipei), 2006, vol. 58, no. 1, p. 35–40. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

Isoquercitrin (RF-00010712-PAR)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

isoquercitrin in aerial parts

Composition #8: Boalino, DM., McLean, S., Reynolds, WF., Tinto, WF. Labdane diterpenes of Leonurus sibiricus. journal of natural products, 2004, vol. 67, no. 4, p. 714–717. DOI: 10.1021/np030480i. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

sibiricinones A-E and 15-epi-sibiricinones D and E
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

sibiricinones A-E and 15-epi-sibiricinones D and E in aerial parts

Composition #9: Moon, Hyun Teak., Jin, Qinglong., Shin, Ji Eun., Choi, Eun Jin., Han, Hyo-Kyung., Kim, Yeong Shik., Woo, Eun-Rhan. Bis-spirolabdane-Type Diterpenoids from Leonurus sibiricus. journal of natural products, 2010, vol. 73, no. 2, p. 123–126. DOI: 10.1021/np900471x. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

bis-spirolabdane-type diterpenoids (leosibirinone A, 3R-acetoxyleoheteronone C, leosibirinone B, 3R-hydroxyleoheteronone A, 3R-acetoxyleoheteronone E, and 3R-acetoxy-15-epileoheteronone E)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

labdane diterpens: bis-spirolabdane-type diterpenoids (leosibirinone A, 3R-acetoxyleoheteronone C, leosibirinone B, 3R-hydroxyleoheteronone A, 3R-acetoxyleoheteronone E, and 3R-acetoxy-15-epileoheteronone E) in aerial parts

Composition #10: Pitschmann, Anna., Zehl, Martin., Heiss, Elke., Purevsuren, Sodnomtseren., Urban, Ernst., Dirsch, Verena M., Glasl, Sabine. Quantitation of phenylpropanoids and iridoids in insulin-sensitising extracts of Leonurus sibiricus L. (Lamiaceae) . phytochemical analysis, 2016, vol. 27, no. 1, p. 23–31. DOI: 10.1002/pca.2583. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol / Methanol - water / butanol
Substance:

phenylpropanoids (RF-00004367-PAR)
Analytical method code:

HPLC-CAD (F328A)
Analytical method - additional description:

HPLC-CAD and HPLC-DAD-MS analyses
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.58 - 13.01
Internal remarks:

phenylpropanoids in aerial parts

Composition #11: Sitarek, Przemyslaw., Skala, Ewa., Toma, Monika., Wielanek, Marzena., Szemraj, Janusz., Nieborowska-Skorska, Malgorzata., Kolasa, Maciej., Skorski, Tomasz., Wysokinska, Halina., Sliwinski, Tomasz. A preliminary study of apoptosis induction in glioma cells via alteration of the Bax/Bcl-2-p53 axis by transformed and non-transformed root extracts of Leonurus sibiricus L. . tumor biology, 2016, vol. 37, no. 7, p. 8753–8764. DOI: 10.1007/s13277-015-4714-2. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol - water
Substance:

p-coumaric acid (RF-00010717-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/gram (G060A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.0526
Internal remarks:

p-Coumaric acid in roots

Composition #12: Zachow, Lucimara L., Avila, Janaina M., Saldanha, Geovane A., Mostardeiro, Marco A., da Silva, Ubiratan F., Morel, Ademir F., Dalcol, Ionara I. Chemical composition and evaluation of prolyl oligopeptidase and acetylcholinesterase inhibitory activities of Leonurus Sibiricus L. from Brazil . natural product research, 2017, vol. 31, no. 12, p. 1459–1463. DOI: 10.1080/14786419.2016.1255890. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

leojaponin
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

leojaponin in aerial parts

Composition #13: Zachow, Lucimara L., Avila, Janaina M., Saldanha, Geovane A., Mostardeiro, Marco A., da Silva, Ubiratan F., Morel, Ademir F., Dalcol, Ionara I. Chemical composition and evaluation of prolyl oligopeptidase and acetylcholinesterase inhibitory activities of Leonurus Sibiricus L. from Brazil . natural product research, 2017, vol. 31, no. 12, p. 1459–1463. DOI: 10.1080/14786419.2016.1255890. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

Leonurine
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

HPLC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Leonurine in aerial parts