EFSA Compendium of Botanicals

Draft - work in progress

Leonurus cardiaca L.

Family:: Lamiaceae
Approval state:: Approved
Number of articles retrieved:: 326
Number of processed references:: 7

Compositions

#1: Labdane diterpenes (leosibiricin, preleosibirin, leosibirin and isoballotenol acetate) in shoots #2: Labdane diterpene ( 19-acetoxypregaleopsin) in aerial part #3: Labdane diterpenes (mainly leosibiricin) in root #4: Labdane diterpenes (mainly leosibiricin) in flowers #5: Labdane diterpenes (mainly leosibiricin) in leaves #6: Labdane diterpenes (mainly leosibiricin) in stem #7: dehydro-1,8-cineole in essential oil #8: Stachydrine in aerial parts #9: stachydrine in aerial parts (with fruit) #10: Labdan diterpene (isoleosibirin) in aerial part #11: Stachydrine in aerial parts

Endpoint Studies

Genotoxicity

Composition #1: Knoess, Werner. Furanic labdane diterpenes in differentiated and undifferentiated cultures of Marrubium vulgare and Leonurus cardiaca . plant physiology and biochemistry (montrouge), 1994, vol. 32, no. 6, p. 785–789. .

Language:

English (en)
Plant part:

Shoot (as part-nature) (A067T)
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

leosibiricin, preleosibirin, leosibirin and isoballotenol acetate
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Labdane diterpenes (leosibiricin, preleosibirin, leosibirin and isoballotenol acetate) in shoots

Composition #2: Papanov, Georgi Y., Malakov, Peter Y., Rodriguez, Benjamin., De La Torre, Maria C. A prefuranic labdane diterpene from Leonurus cardiaca. phytochemistry (oxford), 1998, vol. 47, no. 6, p. 1149–1151. DOI: 10.1016/S0031-9422(97)00415-9. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetone
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

19-acetoxypregaleopsin
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

IR, MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Labdane diterpene ( 19-acetoxypregaleopsin) in aerial part

Composition #3: Knoss, W., Zapp, J. Accumulation of furanic labdane diterpenes in Marrubium vulgare and Leonurus cardiaca . planta medica, 1998, vol. 64, no. 4, p. 357–361. DOI: 10.1055/s-2006-957451. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetone
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

mainly leosibiricin, premarrublin, marrublin
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

TLC, photometry
Result unit:

Milligram/gram (G060A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.01-0.05
Internal remarks:

Labdane diterpenes (mainly leosibiricin) in root

Composition #4: Knoss, W., Zapp, J. Accumulation of furanic labdane diterpenes in Marrubium vulgare and Leonurus cardiaca . planta medica, 1998, vol. 64, no. 4, p. 357–361. DOI: 10.1055/s-2006-957451. [DOI link]

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetone
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

mainly leosibiricin, premarrublin, marrublin
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

TLC, photometry
Result unit:

Milligram/gram (G060A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.4-3.9
Internal remarks:

Labdane diterpenes (mainly leosibiricin) in flowers

Composition #5: Knoss, W., Zapp, J. Accumulation of furanic labdane diterpenes in Marrubium vulgare and Leonurus cardiaca . planta medica, 1998, vol. 64, no. 4, p. 357–361. DOI: 10.1055/s-2006-957451. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetone
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

mainly leosibiricin, premarrublin, marrublin
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

TLC, photometry
Result unit:

Milligram/gram (G060A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.8-3.4
Internal remarks:

Labdane diterpenes (mainly leosibiricin) in leaves

Composition #6: Knoss, W., Zapp, J. Accumulation of furanic labdane diterpenes in Marrubium vulgare and Leonurus cardiaca . planta medica, 1998, vol. 64, no. 4, p. 357–361. DOI: 10.1055/s-2006-957451. [DOI link]

Language:

English (en)
Plant part:

Stem / stalk (as part-nature) (A067S)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetone
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

mainly leosibiricin, premarrublin, marrublin
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

TLC, photometry
Result unit:

Milligram/gram (G060A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.4-0.6
Internal remarks:

Labdane diterpenes (mainly leosibiricin) in stem

Composition #7: Morteza-Semnani, Katayoun., Saeedi, Majid., Akbarzadeh, Mohammad. The essential oil composition of Leonurus cardiaca L.. journal of essential oil research, 2008, vol. 20, no. 2, p. 107–109. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

hydrodistillation
Substance:

Dehydro-1,8-cineole (RF-00011478-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

8.9
Internal remarks:

dehydro-1,8-cineole in essential oil

Composition #8: Ritter, Malte., Melichar, Kerstin., Strahler, Sabine., Kuchta, Kenny., Schulte, Jan., Sartiani, Laura., Cerbai, Elisabetta., Mugelli, Alessandro., Mohr, Friedrich-Wilhelm., Rauwald, Hans Wilhelm., Dhein, Stefan. Cardiac and Electrophysiological Effects of Primary and Refined Extracts from Leonurus cardiaca L. (Ph.Eur.) . planta medica, 2010, vol. 76, no. 6, p. 572–582. DOI: 10.1055/s-0029-1240602. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

dichloromethane
Substance:

stachydrine (RF-00004062-PAR)
Analytical method code:

HPLC-DAD (F587A)
Analytical method - additional description:

TLC
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.212
Internal remarks:

Stachydrine in aerial parts

Composition #9: Kuchta, Kenny., Ortwein, Jutta., Hennig, Lothar., Wilhelm-Rauwald, Hans. H-1-qNMR for direct quantification of stachydrine in Leonurus japonicus and L. cardiaca . fitoterapia, 2014, vol. 96, p. 8–17. DOI: 10.1016/j.fitote.2014.03.023. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

aerial parts with fruits, dried, pulverised and extracted with boiling water
Substance:

stachydrine (RF-00004062-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Result unit:

Percent (G138A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.12-1.55
Internal remarks:

stachydrine in aerial parts (with fruit)

Composition #10: Rauwald, Hans Wilhelm., Savtschenko, Alex., Merten, Alexander., Rusch, Christian., Appel, Kurt., Kuchta, Kenny. GABA(A) Receptor Binding Assays of Standardized Leonurus cardiaca and Leonurus japonicus Extracts as Well as Their Isolated Constituents . planta medica, 2015, vol. 81, no. 12-13, p. 1103–1110. DOI: 10.1055/s-0035-1546234. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Pulverized drug material was extracted with 45% ethanol
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

isoleosibirin
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

EIMS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Labdan diterpene (isoleosibirin) in aerial part

Composition #11: Rauwald, Hans Wilhelm., Savtschenko, Alex., Merten, Alexander., Rusch, Christian., Appel, Kurt., Kuchta, Kenny. GABA(A) Receptor Binding Assays of Standardized Leonurus cardiaca and Leonurus japonicus Extracts as Well as Their Isolated Constituents . planta medica, 2015, vol. 81, no. 12-13, p. 1103–1110. DOI: 10.1055/s-0035-1546234. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Pulverized drug material was extracted with 45% ethanol
Substance:

stachydrine (RF-00004062-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Stachydrine in aerial parts