EFSA Compendium of Botanicals

Draft - work in progress

Chickling vetch (as plant) // Lathyrus sativus L.

Family:: Fabaceae
Approval state:: Approved
Number of articles retrieved:: 2066
Number of processed references:: 5

Compositions

#1: trypsin inhibitor in raw seeds #2: beta-ODPA in raw seeds #3: phytic acid in seeds #4: Phytate in seeds #5: ODAP in seeds #6: beta-ODAP in seeds

Endpoint Studies

#1: 45 individuals 25-35 years after intake of 400g peas daily for three months, skeletal changes identified: absence of ossification centers of the iliac creasts, ischial tuberosis and vertebrae; bowing with thickening of the femoral shaft also occurred. #2: neuropathological findings and behavioral changes after BOOA from seed in monkeys

Genotoxicity

Composition #1: Ramachandran, S., Ray, A.K. Effect of different processing techniques on the nutritive value of grass pea, lathyrus sativus l., seed meal in compound diets for indian major carp rohu, labeo rohita (hamilton), fingerlings. archives of polish fisheries, 2008, vol. 16, no. 2, p. 189–202. DOI: 10.2478/s10086-008-0016-2. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Powder (A06JD)
Additional extraction description:

meal from raw seeds
Substance:

Trypsin inhibitors Trypsin inhibitors (RF-00004339-PAR)
Analytical method - additional description:

method of Kakade
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

30.49-32.35
Internal remarks:

trypsin inhibitor in raw seeds

Composition #2: Ramachandran, S., Ray, A.K. Effect of different processing techniques on the nutritive value of grass pea, lathyrus sativus l., seed meal in compound diets for indian major carp rohu, labeo rohita (hamilton), fingerlings. archives of polish fisheries, 2008, vol. 16, no. 2, p. 189–202. DOI: 10.2478/s10086-008-0016-2. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Powder (A06JD)
Additional extraction description:

raw peas
Substance:

beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP) (RF-00004108-PAR)
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

spectrophotometry method
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.23-1.33
Internal remarks:

beta-ODPA in raw seeds

Composition #3: Ramachandran, S., Ray, A.K. Effect of different processing techniques on the nutritive value of grass pea, lathyrus sativus l., seed meal in compound diets for indian major carp rohu, labeo rohita (hamilton), fingerlings. archives of polish fisheries, 2008, vol. 16, no. 2, p. 189–202. DOI: 10.2478/s10086-008-0016-2. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Powder (A06JD)
Additional extraction description:

meal from raw seeds
Substance:

phytic acid (RF-00000363-NTR)
Analytical method - additional description:

method according Wheeler and Ferrel
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

6.29-6.75
Internal remarks:

phytic acid in seeds

Composition #4: Hanbury, CD., White, CL., Mullan, BP., Siddique, KHM. A review of the potential of Lathyrus sativus L. and L. cicera L. grain for use as animal feed . animal feed science and technology, 2000, vol. 87, no. 1-2, p. 1–27. DOI: 10.1016/S0377-8401(00)00186-3. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Additional extraction description:

review, not specified
Substance:

phytic acid (RF-00000363-NTR)
Additional substance description:

analysed as phytate
Analytical method code:

Unspecified (F598A)
Analytical method - additional description:

review
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.49-1.09
Internal remarks:

Phytate in seeds

Composition #5: Hanbury, CD., White, CL., Mullan, BP., Siddique, KHM. A review of the potential of Lathyrus sativus L. and L. cicera L. grain for use as animal feed . animal feed science and technology, 2000, vol. 87, no. 1-2, p. 1–27. DOI: 10.1016/S0377-8401(00)00186-3. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Additional extraction description:

review, not specified
Substance:

beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP) (RF-00004108-PAR)
Analytical method code:

Unspecified (F598A)
Analytical method - additional description:

review
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.04-1.5
Internal remarks:

ODAP in seeds

Composition #6: Xing, GM., Wang, F., Cui, KR., Li, ZX. Assay of neurotoxin beta-ODAP and non-protein amino acids in Lathyrus sativus by high-performance liquid chromatography with dansylation . analytical letters, 2001, vol. 34, no. 15, p. 2649–2657. DOI: 10.1081/AL-100108411. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol and water (3:7, v/v)
Substance:

beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP) (RF-00004108-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.3-0.45
Internal remarks:

beta-ODAP in seeds

Endpoint study #1: Cohn, D F., Streifler, M. Intoxication by the chickling pea (Lathyrus sativus): nervous system and skeletal findings. . archives of toxicology. supplement. = archiv fur toxikologie. supplement, 1983, vol. 6, p. 190–3. .

Study relevance:

Human health
Plant part:

Seed (as part-nature) (A066P)
Additional plant/substance description:

cooked in water as daily diet
Type of toxicological test:

case report (CHD060TT)
Tested organism:

Human (as organism) (A056J)
Sex:

Male/Female
Route of exposure:

ORAL: UNSPECIFIED (OECD2234PL)
Exposure duration:

3.0
Unit of exposure duration:

Month (G206A)
Number of organisms dosed:

45
Control groups used:

no data
Endpoint measured:

Dose level (CHD014EP)
Concentration unit:

Grams per day (G233A)
Effect concentration:

400
Critical effect:

MUSCLO-SKELETAL (TOX13A)
Target tissue:

Muscle, Skeletal (TT055A)
Effect description:

45 individuals 25-35 years after intake of 400g peas daily for 3 months; skeletal findings: absence of ossification centers of the iliac creasts, ischial tuberosities and vertebrae; bowing with thickening of the femoral shaft also occurred.
Effect or parameter linked to the endpoint:

other (CHD003BE)
Internal remarks:

45 individuals 25-35 years after intake of 400g peas daily for three months, skeletal changes identified: absence of ossification centers of the iliac creasts, ischial tuberosis and vertebrae; bowing with thickening of the femoral shaft also occurred.

Endpoint study #2: Spencer, PS. Food toxins, AMPA receptors, and motor neuron diseases. drug metabolism reviews, 1999, vol. 31, no. 3, p. 561–587. DOI: 10.1081/DMR-100101936. [DOI link]

Study relevance:

Human health
Substance:

beta-N-oxalylamino-L-alanine (BOAA) (RF-00004109-PAR)
Plant part:

Seed (as part-nature) (A066P)
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

unspecified
Type of toxicological test:

subchronic (CHD013TT)
Limit test:

Not applicable
Tested organism:

Rhesus monkey (as animal) (A0CNQ)
Sex:

No data
Exposure duration:

3.0
Unit of exposure duration:

Month (G206A)
Control groups used:

yes, plain diet
Endpoint measured:

Dose level (CHD014EP)
Concentration unit:

Grams per kilogram body weight per day (G212A)
Effect concentration:

1.1-1.5
Critical effect:

NEUROTOXICITY (TOX04A)
Target tissue:

Muscle, Skeletal (TT055A)
Effect description:

neuropathological findings (increased muscle tone, reflex activity, crossed-adductor response), behavioral changes
Effect or parameter linked to the endpoint:

neurology (CHD027BE)
Internal remarks:

neuropathological findings and behavioral changes after BOOA from seed in monkeys