EFSA Compendium of Botanicals

Draft - work in progress

Isatis tinctoria L. // Isatis indigotica Fortune ex Lindl.

Family:: Brassicaceae
Approval state:: Approved
Number of articles retrieved:: 1817
Number of processed references:: 4

Compositions

#1: Salicylic acid in roots #2: Glucoibervirin (a glucosinolate) in leaves #3: Total Glucosinolates in roots #4: Indole alkaloids in roots #5: lariciresinol-4-O D glucopyranoside in roots

Endpoint Studies

Genotoxicity

Composition #1: Li, Jing., Liu, Yunhai., Tang, Jie., Xie, Wei. Anti-endotoxic effects of salicylic acid from Radix isatidis. zhongguo yiyuan yaoxue zazhi, 2007, vol. 27, no. 10, p. 1349–1352. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

Salicylic acid (RF-00000241-VET)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Salicylic acid in roots

Composition #2: Hu, Ye., Liang, Hao., Yuan, Qipeng., Hong, Yuancheng. Determination of glucosinolates in 19 Chinese medicinal plants with spectrophotometry and high-pressure liquid chromatography. natural product research, 2010, vol. 24, no. 13, p. 1195–1205. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Deionised water
Substance:

Glucoibervirin (RF-00013335-PAR)
Analytical method code:

HPLC-UV (F021A)
Result unit:

Millimole/kilogram (G070A)
Result expression:

Dry matter (B002A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.824
Internal remarks:

Glucoibervirin (a glucosinolate) in leaves

Composition #3: Hu, Ye., Liang, Hao., Yuan, Qipeng., Hong, Yuancheng. Determination of glucosinolates in 19 Chinese medicinal plants with spectrophotometry and high-pressure liquid chromatography. natural product research, 2010, vol. 24, no. 13, p. 1195–1205. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Deionised water
Substance:

Sum of glucosinolates Sum of glucosinolates (RF-00000331-TOX)
Additional substance description:

"Total Glucosinolates"
Analytical method code:

HPLC-UV (F021A)
Result unit:

Millimole/kilogram (G070A)
Result expression:

Dry matter (B002A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

49.973
Internal remarks:

Total Glucosinolates in roots

Composition #4: Yang, Liguo., Wang, Guan., Wang, Meng., Jiang, Hongmei., Chen, Lixia., Zhao, Feng., Qiu, Feng. Indole alkaloids from the roots of Isatis indigotica and their inhibitory effects on nitric oxide production . fitoterapia, 2014, vol. 95, p. 175–181. DOI: 10.1016/j.fitote.2014.03.019. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70% EtOH
Substance:

indole alkaloids (RF-00004503-PAR)
Additional substance description:

14 compounds isolated (3 new), all indole alkaloids
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Also: UV spec, IR spec, HRESIMS, and NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Indole alkaloids in roots

Composition #5: Li, Jing., Zhou, Beixian., Li, Chufang., Chen, QiaoYan., Wang, Yutao., Li, Zhengtu., Chen, Tingting., Yang, Chunguang., Jiang, Zhihong., Zhong, Nanshan., Yang, Zifeng., Chen, Rongchang. Lariciresinol-4-O-beta-D-glucopyranoside from the root of Isatis indigotica inhibits influenza A virus-induced pro-inflammatory response . journal of ethnopharmacology, 2015, vol. 174, p. 379–386. DOI: 10.1016/j.jep.2015.08.037. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

95% ethanol
Substance:

lariciresinol (RF-00011086-PAR)
Additional substance description:

"lariciresinol-4-O D glucopyranoside", a derivative of lariciresinol, also a lignan glycoside
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

Also: NMR spec
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lariciresinol-4-O D glucopyranoside in roots