EFSA Compendium of Botanicals

Draft - work in progress

Heracleum sphondylium L. // Heracleum sphondylium L. subsp. sibiricum (L.) Simonk.

Family:: Apiaceae
Approval state:: Approved
Number of articles retrieved:: 433
Number of processed references:: 8

Endpoint Studies

Genotoxicity

#1: Photogenotoxic effect of isopimpinellin

Composition #1: Fischer, Frederik C., Svendsen, A. Baerheim. Apterin, a common furanocoumarin glycoside in the umbelliferae. phytochemistry (elsevier), 1976, vol. 15, no. 6, p. 1079–80. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

reference to the authors previous published method
Substance:

apterin (RF-00004152-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

also UV-spectroscopy
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Apterin in roots

Composition #2: Glowniak, K., Matysik, G., Bieganowska, M., Soczewinski, E. Gradient thin-layer chromatography of coumarins and furocoumarins. chromatographia, 1986, vol. 22(7-12), p. 307–10. .

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

petroleum ether
Substance:

osthol (RF-00011008-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Osthol in seeds

Composition #3: Glowniak, K., Matysik, G., Bieganowska, M., Soczewinski, E. Gradient thin-layer chromatography of coumarins and furocoumarins. chromatographia, 1986, vol. 22(7-12), p. 307–10. .

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

petroleum ether
Substance:

umbelliferone (RF-00004096-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Umbelliferone in seeds

Composition #4: Wawrzynowicz, T., Waksmundzka-Hajnos, M., Bieganowska, M. L. Chromatographic investigations of furocoumarins from Heracleum genus fruits. chromatographia, 1989, vol. 28(3-4), p. 161–6. .

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether
Substance:

imperatorin (RF-00003874-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Imperatorin (fruit)

Composition #5: Wawrzynowicz, T., Waksmundzka-Hajnos, M., Bieganowska, M. L. Chromatographic investigations of furocoumarins from Heracleum genus fruits. chromatographia, 1989, vol. 28(3-4), p. 161–6. .

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether
Substance:

Isopimpinellin (RF-00003784-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isopimpinellin (fruit)

Composition #6: Wawrzynowicz, T., Waksmundzka-Hajnos, M., Bieganowska, M. L. Chromatographic investigations of furocoumarins from Heracleum genus fruits. chromatographia, 1989, vol. 28(3-4), p. 161–6. .

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether
Substance:

Phellopterin (RF-00003560-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Phellopterin (fruit)

Composition #7: Wawrzynowicz, T., Waksmundzka-Hajnos, M., Bieganowska, M. L. Chromatographic investigations of furocoumarins from Heracleum genus fruits. chromatographia, 1989, vol. 28(3-4), p. 161–6. .

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether
Substance:

Xanthotoxol (RF-00011114-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Xanthotoxol (fruit)

Composition #8: Wawrzynowicz, T., Waksmundzka-Hajnos, M., Bieganowska, M. L. Chromatographic investigations of furocoumarins from Heracleum genus fruits. chromatographia, 1989, vol. 28(3-4), p. 161–6. .

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether
Substance:

Bergapten (RF-00003837-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Bergapten (fruit)

Composition #9: Ozek, T., Ozek, G., Baser, KHC., Duran, A. Comparison of the essential oils of three endemic Turkish Heracleum species obtained by different isolation techniques . journal of essential oil research, 2005, vol. 17, no. 6, p. 605–610. .

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

Hydro distillation, micro-distillation, micro steam distilled, solid phase micro extraction, microwave assisted hydrodistillation
Substance:

apiole (RF-00003690-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

4.7-20.4
Internal remarks:

apiole (fruits)

Composition #10: Ciesla, Lukasz., Bogucka-Kocka, Anna., Hajnos, Michal., Petruczynik, Anna., Waksmundzka-Hajnos, Monika. Two-dimensional thin-layer chromatography with adsorbent gradient as method of chromatographic fingerprinting of furanocoumarins for distinguishing selected varieties and forms of Heracleum spp. . journal of chromatography a, 2008, vol. 1207, no. 1-2, p. 160–168. DOI: 10.1016/j.chroma.2008.08.065. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum Ether
Substance:

Phellopterin (RF-00003560-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Phellopterin (roots)

Composition #11: Ciesla, Lukasz., Bogucka-Kocka, Anna., Hajnos, Michal., Petruczynik, Anna., Waksmundzka-Hajnos, Monika. Two-dimensional thin-layer chromatography with adsorbent gradient as method of chromatographic fingerprinting of furanocoumarins for distinguishing selected varieties and forms of Heracleum spp. . journal of chromatography a, 2008, vol. 1207, no. 1-2, p. 160–168. DOI: 10.1016/j.chroma.2008.08.065. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether
Substance:

Bergapten (RF-00003837-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Bergapten (roots)

Composition #12: Ciesla, Lukasz., Bogucka-Kocka, Anna., Hajnos, Michal., Petruczynik, Anna., Waksmundzka-Hajnos, Monika. Two-dimensional thin-layer chromatography with adsorbent gradient as method of chromatographic fingerprinting of furanocoumarins for distinguishing selected varieties and forms of Heracleum spp. . journal of chromatography a, 2008, vol. 1207, no. 1-2, p. 160–168. DOI: 10.1016/j.chroma.2008.08.065. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum Ether
Substance:

Byakangelicol (RF-00011117-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Byakangelicol (roots)

Composition #13: Ciesla, Lukasz., Bogucka-Kocka, Anna., Hajnos, Michal., Petruczynik, Anna., Waksmundzka-Hajnos, Monika. Two-dimensional thin-layer chromatography with adsorbent gradient as method of chromatographic fingerprinting of furanocoumarins for distinguishing selected varieties and forms of Heracleum spp. . journal of chromatography a, 2008, vol. 1207, no. 1-2, p. 160–168. DOI: 10.1016/j.chroma.2008.08.065. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum Ether
Substance:

Bergapten (RF-00003837-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Bergapten (fruit)

Composition #14: Ciesla, Lukasz., Bogucka-Kocka, Anna., Hajnos, Michal., Petruczynik, Anna., Waksmundzka-Hajnos, Monika. Two-dimensional thin-layer chromatography with adsorbent gradient as method of chromatographic fingerprinting of furanocoumarins for distinguishing selected varieties and forms of Heracleum spp. . journal of chromatography a, 2008, vol. 1207, no. 1-2, p. 160–168. DOI: 10.1016/j.chroma.2008.08.065. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum Ether
Substance:

Isopimpinellin (RF-00003784-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isopimpinellin (roots)

Composition #15: Ciesla, Lukasz., Bogucka-Kocka, Anna., Hajnos, Michal., Petruczynik, Anna., Waksmundzka-Hajnos, Monika. Two-dimensional thin-layer chromatography with adsorbent gradient as method of chromatographic fingerprinting of furanocoumarins for distinguishing selected varieties and forms of Heracleum spp. . journal of chromatography a, 2008, vol. 1207, no. 1-2, p. 160–168. DOI: 10.1016/j.chroma.2008.08.065. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum Ether
Substance:

heraclenin (RF-00011110-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Heraclenin (roots)

Composition #16: Ciesla, Lukasz., Bogucka-Kocka, Anna., Hajnos, Michal., Petruczynik, Anna., Waksmundzka-Hajnos, Monika. Two-dimensional thin-layer chromatography with adsorbent gradient as method of chromatographic fingerprinting of furanocoumarins for distinguishing selected varieties and forms of Heracleum spp. . journal of chromatography a, 2008, vol. 1207, no. 1-2, p. 160–168. DOI: 10.1016/j.chroma.2008.08.065. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum Ether
Substance:

Isopimpinellin (RF-00003784-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isopimpinellin (fruits)

Composition #17: Ciesla, Lukasz., Petruczynik, Anna., Hajnos, Michal., Bogucka-Kocka, Anna., Waksmundzka-Hajnos, Monika. Two-Dimensional Thin-Layer Chromatography of Structural Analogs. Part II. Method for Quantitative Analysis of Selected Coumarins in Plant Material . jpc-journal of planar chromatography-modern tlc, 2008, vol. 21, no. 6, p. 447–452. DOI: 10.1556/JPC.21.2008.6.10. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether
Substance:

Byakangelicol (RF-00011117-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

4.13
Internal remarks:

Byakangelicol (fruit)

Composition #18: Ciesla, Lukasz., Petruczynik, Anna., Hajnos, Michal., Bogucka-Kocka, Anna., Waksmundzka-Hajnos, Monika. Two-Dimensional Thin-Layer Chromatography of Structural Analogs. Part II. Method for Quantitative Analysis of Selected Coumarins in Plant Material . jpc-journal of planar chromatography-modern tlc, 2008, vol. 21, no. 6, p. 447–452. DOI: 10.1556/JPC.21.2008.6.10. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether
Substance:

Bergapten (RF-00003837-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.73-2.63
Internal remarks:

Bergapten (fruit)

Composition #19: Ciesla, Lukasz., Petruczynik, Anna., Hajnos, Michal., Bogucka-Kocka, Anna., Waksmundzka-Hajnos, Monika. Two-Dimensional Thin-Layer Chromatography of Structural Analogs. Part II. Method for Quantitative Analysis of Selected Coumarins in Plant Material . jpc-journal of planar chromatography-modern tlc, 2008, vol. 21, no. 6, p. 447–452. DOI: 10.1556/JPC.21.2008.6.10. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum Ether
Substance:

Phellopterin (RF-00003560-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.88-1.07
Internal remarks:

Phellopterin (fruit)

Composition #20: ABEL, G., ERDELMEIER, C., MEIER, B., STICHER, O. ISOPIMPINELLIN A FUROCOUMARIN FROM HERACLEUM-SPHONDYLIUM WITH CHROMOSOME DAMAGING ACTIVITY . planta medica, 1985, vol. 51, no. 3, p. 250–252. DOI: 10.1055/s-2007-969470. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

pimpinellin (RF-00003997-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Pimpinellin in roots

Composition #21: ABEL, G., ERDELMEIER, C., MEIER, B., STICHER, O. ISOPIMPINELLIN A FUROCOUMARIN FROM HERACLEUM-SPHONDYLIUM WITH CHROMOSOME DAMAGING ACTIVITY . planta medica, 1985, vol. 51, no. 3, p. 250–252. DOI: 10.1055/s-2007-969470. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

sphondin (RF-00004060-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Sphondin in roots

Composition #22: ABEL, G., ERDELMEIER, C., MEIER, B., STICHER, O. ISOPIMPINELLIN A FUROCOUMARIN FROM HERACLEUM-SPHONDYLIUM WITH CHROMOSOME DAMAGING ACTIVITY . planta medica, 1985, vol. 51, no. 3, p. 250–252. DOI: 10.1055/s-2007-969470. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

isobergapten (RF-00003441-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isobergapten in roots

Composition #23: ABEL, G., ERDELMEIER, C., MEIER, B., STICHER, O. ISOPIMPINELLIN A FUROCOUMARIN FROM HERACLEUM-SPHONDYLIUM WITH CHROMOSOME DAMAGING ACTIVITY . planta medica, 1985, vol. 51, no. 3, p. 250–252. DOI: 10.1055/s-2007-969470. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

Isopimpinellin (RF-00003784-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isopimpinellin in roots

Composition #24: ABEL, G., ERDELMEIER, C., MEIER, B., STICHER, O. ISOPIMPINELLIN A FUROCOUMARIN FROM HERACLEUM-SPHONDYLIUM WITH CHROMOSOME DAMAGING ACTIVITY . planta medica, 1985, vol. 51, no. 3, p. 250–252. DOI: 10.1055/s-2007-969470. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

Bergapten (RF-00003837-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Bergapten in roots

Composition #25: BORGKARLSON, AK., VALTEROVA, I., NILSSON, LA. VOLATILE COMPOUNDS FROM FLOWERS OF 6 SPECIES IN THE FAMILY APIACEAE - BOUQUETS FOR DIFFERENT POLLINATORS . phytochemistry, 1994, vol. 35, no. 1, p. 111–119. .

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Substance:

myrcene (RF-00003503-PAR)
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<10
Internal remarks:

Myrcene (flower)

Genotox #1: ABEL, G., ERDELMEIER, C., MEIER, B., STICHER, O. ISOPIMPINELLIN A FUROCOUMARIN FROM HERACLEUM-SPHONDYLIUM WITH CHROMOSOME DAMAGING ACTIVITY . planta medica, 1985, vol. 51, no. 3, p. 250–252. DOI: 10.1055/s-2007-969470. [DOI link]

Study category:

Genotoxicity
Type of toxicological test:

sister chromatid exchange assay in mammalian cells (CHD039TT)
Method type:

in vitro
Tested organism:

Human (as organism) (A056J)
Strain of test organism:

lymphocytes (CHD036ST)
Exposure duration for in vivo:

15.0
Unit of exposure duration:

Minute (G074A)
Endpoint measured:

DNA damage and/or repair
Is genotoxic:

Positive
Remarks on genotox study:

A weak clastogenic effect after UV-A exposure -photogenotoxic effect
Internal remarks:

Photogenotoxic effect of isopimpinellin
Internal ID:

WOS:A1985ALS7900020