EFSA Compendium of Botanicals

Draft - work in progress

Geissospermum laeve (Vell.) Miers // Geissospermum vellosii Allem.

Family:: Apocynaceae
Approval state:: Approved
Number of articles retrieved:: 66
Number of processed references:: 6

Compositions

#1: beta-carboline alkaloids in bark #2: Geissospermine in stem bark #3: Alkaloids derivatives in bark (leuconolam, Geissolosimine, Geissospermine, geissolaevine) #4: Flavopereirine in bark #5: Indole alkaloids (vellosimine, vellosiminol, geissolosimine) in plant #6: Geissoschizoline in plant #7: Geissovelline, an indole alkaloid, in bark

Endpoint Studies

Genotoxicity

Composition #1: Bemis, Debra L., Capodice, Jillian L., Desai, Manisha., Katz, Aaron E., Buttyan, Ralph. beta-carboline alkaloid-enriched extract from the amazonian rain forest tree pao pereira suppresses prostate cancer cells. . journal of the society for integrative oncology, 2009, vol. 7, no. 2, p. 59–65. .

Language:

English (en)
Plant part:

Bark (as part-nature) (A066X)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

carboline alkaloids (RF-00004384-PAR)
Additional substance description:

beta-carboline alkaloids
Analytical method code:

Unspecified (F598A)
Analytical method - additional description:

Compound group mentioned in paper, but not identified specifically in this study. No methods details.
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

beta-carboline alkaloids in bark

Composition #2: Lima, Joselia A., Costa, Rodrigo Saar., Epifanio, Rosangela A., Castro, Newton G., Rocha, Monica S., Pinto, Angelo C. Geissospermum vellosii stembark Anticholinesterase activity and improvement of scopolamine-induced memory deficits . pharmacology biochemistry and behavior, 2009, vol. 92, no. 3, p. 508–513. DOI: 10.1016/j.pbb.2009.01.024. [DOI link]

Language:

English (en)
Plant part:

Bark (as part-nature) (A066X)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol, then water/hexane and ethyl acetate
Substance:

geissospermine (RF-00003826-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

TLC separation
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Geissospermine in stem bark

Composition #3: Ramos, Alexander E. Fox., Alcover, Charlotte., Evanno, Laurent., Maciuk, Alexandre., Litaudon, Marc., Duplais, Christophe., Bernadat, Guillaume., Gallard, Jean-Francois., Jullian, Jean-Christophe., Mouray, Elisabeth., Grellier, Philippe., Loiseau, Philippe M., Pomel, Sebastien., Poupon, Erwan., Champy, Pierre., Beniddir, Mehdi A. Revisiting Previously Investigated Plants: A Molecular Networking Based Study of Geissospermum laeve . journal of natural products, 2017, vol. 80, no. 4, p. 1007–1014. DOI: 10.1021/acs.jnatprod.6b01013. [DOI link]

Language:

English (en)
Plant part:

Bark (as part-nature) (A066X)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkalinized with 6 M NH4OH and extracted with CH2Cl2. The CH2Cl2 crude extract was concentrated under vacuum and dissolved in CH2Cl2, then extracted with 0.1M HCl
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

leuconolam, Geissolosimine, Geissospermine, geissolaevine
Analytical method code:

HPLC-MS/MS (F698A)
Result unit:

Percent (G138A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.0013- 0.008
Internal remarks:

Alkaloids derivatives in bark (leuconolam, Geissolosimine, Geissospermine, geissolaevine)

Composition #4: HUGHES, NA., RAPOPORT, H. FLAVOPEREIRINE, AN ALKALOID FROM GEISSOSPERMUM-VELLOSII. journal of the american chemical society, 1958, vol. 80, no. 7, p. 1604–1609. DOI: 10.1021/ja01540a024. [DOI link]

Language:

English (en)
Plant part:

Bark (as part-nature) (A066X)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Fractionation of the total crude alkaloids between ether and water led to a strongly basic fraction, isolated from the aqueous pH solution by extraction at pH10
Substance:

flavopereirine (RF-00003817-PAR)
Analytical method code:

Traditional analytical techniques (wet chemical tests) (F003A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Flavopereirine in bark

Composition #5: RAPOPORT, H., MOORE, RE. ALKALOIDS OF GEISSOSPERMUM VELLOSSI - ISOLATION AND STRUCTURE DETERMINATIONS OF VELLOSIMINE, VELLOSIMINOL, AND GEISSOLOSIMINE . journal of organic chemistry, 1962, vol. 27, no. 9, p. 2981–&. DOI: 10.1021/jo01056a004. [DOI link]

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified for initial extraction - the compounds were separated by chromatography of separate pH fractions
Substance:

indole alkaloids (RF-00004503-PAR)
Additional substance description:

Vellosimine, vellosiminol, geissolosimine
Analytical method code:

Traditional analytical techniques (wet chemical tests) (F003A)
Analytical method - additional description:

Chromatography
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Indole alkaloids (vellosimine, vellosiminol, geissolosimine) in plant

Composition #6: RAPOPORT, H., MOORE, RE. ALKALOIDS OF GEISSOSPERMUM VELLOSSI - ISOLATION AND STRUCTURE DETERMINATIONS OF VELLOSIMINE, VELLOSIMINOL, AND GEISSOLOSIMINE . journal of organic chemistry, 1962, vol. 27, no. 9, p. 2981–&. DOI: 10.1021/jo01056a004. [DOI link]

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified for initial extraction - the compounds were separated by chromatography of separate pH fractions
Substance:

geissoschizoline (RF-00003825-PAR)
Analytical method code:

Traditional analytical techniques (wet chemical tests) (F003A)
Analytical method - additional description:

Chromatography
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Geissoschizoline in plant

Composition #7: MOORE, RE., RAPOPORT, H. GEISSOVELLINE - NEW ALKALOID FROM GEISSOSPERMUM-VELLOSII. journal of organic chemistry, 1973, vol. 38, no. 2, p. 215–230. DOI: 10.1021/jo00942a007. [DOI link]

Language:

English (en)
Plant part:

Bark (as part-nature) (A066X)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

separation of alkaloid fractions with liquid-liquid extractions
Substance:

indole alkaloids (RF-00004503-PAR)
Additional substance description:

Geissovelline an indole alkaloid
Analytical method code:

Traditional analytical techniques (wet chemical tests) (F003A)
Result unit:

Gram (G148A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

9.45
Internal remarks:

Geissovelline, an indole alkaloid, in bark