EFSA Compendium of Botanicals

Draft - work in progress

Digitalis lutea L.

Family:: Plantaginaceae
Approval state:: Approved
Number of articles retrieved:: 73
Number of processed references:: 5

Compositions

#1: Lugrandoside #2: lanatoside C, leaves, paper chromatography #3: cardiac glycosides, leaves, paper chromatography #4: acetyldigitoxin, leaves, paper chromatography #5: digitoxin, leaves, paper chromatography #6: lanatoside A, leaves, paper chromatography #7: lanatoside B, leaves, paper chromatography #8: Digoxin equivalents in leaves (22 microgr per gr fresh weight) #9: Digitoxin equivalents in leaves (500 microgr per gr fresh weight) #10: acetyldigitoxin in powdered leaf #11: glucoverodoxin in powdered leaf #12: glucoevatromonoside in powdered leaf #13: lanatosid B in powdered leaf #14: lanatosid A in powdered leaf #15: lanatoside B, leaves, HPTLC #16: lanatoside A, leaves, HPLC

Endpoint Studies

Genotoxicity

Composition #1: Baudouin, G., Skaltsounis, A L., Tillequin, F., Koch, M. Lugrandoside: a new phenylpropanoid glycoside from various digitalis species. . planta medica, 1988, vol. 54, no. 4, p. 321–3. DOI: 10.1055/s-2006-962445. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

phenylpropanoids (RF-00004367-PAR)
Additional substance description:

Lugrandoside
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Lugrandoside

Composition #2: COLE, JR., GISVOLD, O. A PRELIMINARY PHYTOCHEMICAL INVESTIGATION OF DIGITALIS-LUTEA 1ST YEAR GROWTH . journal of the american pharmaceutical association, 1958, vol. 47, no. 9, p. 654–656. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water
Substance:

lanatoside B (RF-00003895-PAR)
Additional substance description:

lanatoside C
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lanatoside C, leaves, paper chromatography

Composition #3: COLE, JR., GISVOLD, O. A PRELIMINARY PHYTOCHEMICAL INVESTIGATION OF DIGITALIS-LUTEA 1ST YEAR GROWTH . journal of the american pharmaceutical association, 1958, vol. 47, no. 9, p. 654–656. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water
Substance:

cardiac glycosides (RF-00010823-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

cardiac glycosides, leaves, paper chromatography

Composition #4: COLE, JR., GISVOLD, O. A PRELIMINARY PHYTOCHEMICAL INVESTIGATION OF DIGITALIS-LUTEA 1ST YEAR GROWTH . journal of the american pharmaceutical association, 1958, vol. 47, no. 9, p. 654–656. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water
Substance:

acetyldigitoxin (RF-00003663-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

acetyldigitoxin, leaves, paper chromatography

Composition #5: COLE, JR., GISVOLD, O. A PRELIMINARY PHYTOCHEMICAL INVESTIGATION OF DIGITALIS-LUTEA 1ST YEAR GROWTH . journal of the american pharmaceutical association, 1958, vol. 47, no. 9, p. 654–656. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water
Substance:

digitoxin (RF-00003778-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

digitoxin, leaves, paper chromatography

Composition #6: COLE, JR., GISVOLD, O. A PRELIMINARY PHYTOCHEMICAL INVESTIGATION OF DIGITALIS-LUTEA 1ST YEAR GROWTH . journal of the american pharmaceutical association, 1958, vol. 47, no. 9, p. 654–656. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water
Substance:

lanatoside A (RF-00003894-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lanatoside A, leaves, paper chromatography

Composition #7: COLE, JR., GISVOLD, O. A PRELIMINARY PHYTOCHEMICAL INVESTIGATION OF DIGITALIS-LUTEA 1ST YEAR GROWTH . journal of the american pharmaceutical association, 1958, vol. 47, no. 9, p. 654–656. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water
Substance:

lanatoside B (RF-00003895-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lanatoside B, leaves, paper chromatography

Composition #8: HAGIMORI, M., MATSUMOTO, T., KISAKI, T. STUDIES ON THE PRODUCTION OF DIGITALIS CARDENOLIDES BY PLANT-TISSUE CULTURE .1. DETERMINATION OF DIGITOXIN AND DIGOXIN CONTENTS IN 1ST AND 2ND PASSAGE CALLI AND ORGAN RE-DIFFERENTIATING CALLI OF SEVERAL DIGITALIS SPECIES BY RADIOIMMUNOASSAY . plant and cell physiology, 1980, vol. 21, no. 8, p. 1391–1404. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

80% ethanol
Substance:

digoxin (RF-00003779-PAR)
Result unit:

Gram/kilogram (G015A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

22
Internal remarks:

Digoxin equivalents in leaves (22 microgr per gr fresh weight)

Composition #9: HAGIMORI, M., MATSUMOTO, T., KISAKI, T. STUDIES ON THE PRODUCTION OF DIGITALIS CARDENOLIDES BY PLANT-TISSUE CULTURE .1. DETERMINATION OF DIGITOXIN AND DIGOXIN CONTENTS IN 1ST AND 2ND PASSAGE CALLI AND ORGAN RE-DIFFERENTIATING CALLI OF SEVERAL DIGITALIS SPECIES BY RADIOIMMUNOASSAY . plant and cell physiology, 1980, vol. 21, no. 8, p. 1391–1404. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

80% ethanol
Substance:

digitoxin (RF-00003778-PAR)
Result unit:

Gram/kilogram (G015A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

500
Internal remarks:

Digitoxin equivalents in leaves (500 microgr per gr fresh weight)

Composition #10: WICHTL, M., MANGKUDIDJOJO, M., WICHTLBLEIER, W. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF DIGITALIS LEAF EXTRACTS .1. QUALITATIVE-ANALYSIS . journal of chromatography, 1982, vol. 234, no. 2, p. 503–508. DOI: 10.1016/S0021-9673(00)81897-X. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol (70%, v / v)
Substance:

acetyldigitoxin (RF-00003663-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

acetyldigitoxin in powdered leaf

Composition #11: WICHTL, M., MANGKUDIDJOJO, M., WICHTLBLEIER, W. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF DIGITALIS LEAF EXTRACTS .1. QUALITATIVE-ANALYSIS . journal of chromatography, 1982, vol. 234, no. 2, p. 503–508. DOI: 10.1016/S0021-9673(00)81897-X. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol (70%, v / v)
Substance:

glucoverodoxin (RF-00003846-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

glucoverodoxin in powdered leaf

Composition #12: WICHTL, M., MANGKUDIDJOJO, M., WICHTLBLEIER, W. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF DIGITALIS LEAF EXTRACTS .1. QUALITATIVE-ANALYSIS . journal of chromatography, 1982, vol. 234, no. 2, p. 503–508. DOI: 10.1016/S0021-9673(00)81897-X. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol (70%, v / v)
Substance:

glucoevatromonoside (RF-00003842-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

glucoevatromonoside in powdered leaf

Composition #13: WICHTL, M., MANGKUDIDJOJO, M., WICHTLBLEIER, W. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF DIGITALIS LEAF EXTRACTS .1. QUALITATIVE-ANALYSIS . journal of chromatography, 1982, vol. 234, no. 2, p. 503–508. DOI: 10.1016/S0021-9673(00)81897-X. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol (70%, v / v)
Substance:

lanatoside B (RF-00003895-PAR)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lanatosid B in powdered leaf

Composition #14: WICHTL, M., MANGKUDIDJOJO, M., WICHTLBLEIER, W. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF DIGITALIS LEAF EXTRACTS .1. QUALITATIVE-ANALYSIS . journal of chromatography, 1982, vol. 234, no. 2, p. 503–508. DOI: 10.1016/S0021-9673(00)81897-X. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol (70%, v / v)
Substance:

lanatoside A (RF-00003894-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lanatosid A in powdered leaf

Composition #15: FUJII, Y., IKEDA, Y., YAMAZAKI, M. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF LANATOSIDES IN DIGITALIS-LUTEA AND DIGITALIS-AMBIGUA LEAVES . journal of liquid chromatography, 1994, vol. 17, no. 20, p. 4451–4461. DOI: 10.1080/10826079408013629. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform-ethanol
Substance:

lanatoside B (RF-00003895-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPTLC
Result unit:

Microgram/0,01 gram (G045A)
Result expression:

Dry matter (B002A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

43-44.2
Internal remarks:

lanatoside B, leaves, HPTLC

Composition #16: FUJII, Y., IKEDA, Y., YAMAZAKI, M. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF LANATOSIDES IN DIGITALIS-LUTEA AND DIGITALIS-AMBIGUA LEAVES . journal of liquid chromatography, 1994, vol. 17, no. 20, p. 4451–4461. DOI: 10.1080/10826079408013629. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform-ethanol
Substance:

lanatoside A (RF-00003894-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPTLC
Result unit:

Microgram/0,01 gram (G045A)
Result expression:

Dry matter (B002A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

73.2-83.3
Internal remarks:

lanatoside A, leaves, HPLC