EFSA Compendium of Botanicals

Draft - work in progress

Digitalis ciliata Trautv.

Family:: Plantaginaceae
Approval state:: Approved
Number of articles retrieved:: 80
Number of processed references:: 13

Compositions

#1: Gitoxigenin digilanidobioside (leaves) #2: Lanatoside A to C in leaves #3: Cardenolides in leaves #4: digitonine (seed) (quantified) #5: cardiac glycosides (alpha-acetyldigitoxin, digitoxigenin, digitoxin, gitoxigenin) in seeds #6: Digoxin #7: Digitalin #8: Lanatoside A #9: Triterpene saponins in leaves #10: Gitaloxin (leaves) #11: Acetyldigitoxin (leaves) #12: Steroidal glycosides (furostane and 25R-spirostane) (leaves) #13: protodioscin #14: Steroidal furostan saponines (ciliatasides A, B and C) (roots) #15: Cardiac glycosides (digisilicide A, B and C) (roots) #16: Cardiac glycoside (digitoside) (leaves)

Endpoint Studies

Genotoxicity

Composition #1: Kemertelidze, E. P. Glucogitoroside from Digitalis ciliate. khimiya prirodnykh soedinenii, 1971, vol. 7, no. 1, p. 125–6. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Extraction with a mixture of ethanol and chloroform
Substance:

gitoxigenin (RF-00003408-PAR)
Additional substance description:

Gitoxigenin digilanidobioside
Analytical method code:

Chromatographic tests (F015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Gitoxigenin digilanidobioside (leaves)

Composition #2: Kemertelidze, E. P. Digitalinum verum and lanatoside A from Digitalis ciliata. khimiya prirodnykh soedinenii, 1971, vol. (5), p. 673–4. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Unspecified
Substance:

cardenolides (RF-00004389-PAR)
Additional substance description:

lanatosides A, B and C
Analytical method code:

Chromatographic tests (F015A)
Analytical method - additional description:

Chemical tests
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Lanatoside A to C in leaves

Composition #3: Kemertelidze, E. P. Cardenolides of Digitalis ciliata. khimiya prirodnykh soedinenii, 1973, vol. 9, no. 4, p. 563–4. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

not specified
Substance:

cardenolides (RF-00004389-PAR)
Additional substance description:

Digitoxigenin 3-O-bisdigitoxoside, alpha-acetylgitoxin, gitoxin, alpha-acetyldigitoxin, digitoxin, digoxigenin, 3-O-tridigitoxoside, and gitoxigenin-3-O-monodigitaloside
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Cardenolides in leaves

Composition #4: Bylka, Wieslawa., Ellnain-Wojtaszek, Maria., Kowalewski, Zdzislaw., Skrzypczakowa, Lutoslawa. Digitonin content in seeds of some species of the Digitalis genus. herba polonica, 1976, vol. 22(3-4), p. 239–42. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

not specified
Substance:

digitonine (RF-00003777-PAR)
Analytical method code:

UV/VIS spectroscopy (photometry) (F009A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

6.67
Internal remarks:

digitonine (seed) (quantified)

Composition #5: Sulakvelidze, Ts. P., Kemertelidze, E. P. Cardiac glycosides of seeds of Digitalis ciliata. khimiya prirodnykh soedinenii, 1985, vol. (4), p. 577–8. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

not specified
Substance:

cardiac glycosides (RF-00010823-PAR)
Additional substance description:

alpha-acetyldigitoxin, digitoxigenin, digitoxin, gitoxigenin
Analytical method code:

Traditional analytical techniques (wet chemical tests) (F003A)
Analytical method - additional description:

Column of silica gel
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

cardiac glycosides (alpha-acetyldigitoxin, digitoxigenin, digitoxin, gitoxigenin) in seeds

Composition #6: Lichius, J.J., Wichtl, M. Quantitative analysis of cardenolide glycosides in the leaves of Digitalis atlantica, Digitalis ciliata, and Digitalis nervosa. planta medica, 1991, vol. 57, no. SUPPL. 2, p. A73–A74. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Unspecified
Substance:

digoxin (RF-00003779-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

Unspecified
Result unit:

Milligram/100 gram (G057A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

34
Internal remarks:

Digoxin

Composition #7: Lichius, J.J., Wichtl, M. Quantitative analysis of cardenolide glycosides in the leaves of Digitalis atlantica, Digitalis ciliata, and Digitalis nervosa. planta medica, 1991, vol. 57, no. SUPPL. 2, p. A73–A74. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Unspecified
Substance:

Digitalin (RF-00011774-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

94
Internal remarks:

Digitalin

Composition #8: Lichius, J.J., Wichtl, M. Quantitative analysis of cardenolide glycosides in the leaves of Digitalis atlantica, Digitalis ciliata, and Digitalis nervosa. planta medica, 1991, vol. 57, no. SUPPL. 2, p. A73–A74. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Unspecified
Substance:

lanatoside A (RF-00003894-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

146
Internal remarks:

Lanatoside A

Composition #9: Kemertelidze, E.P., Gvazava, L.N., Alaniya, M.D., Kikoladze, V.S. Triterpene glycosides of the leaves of Digitalis ciliata. chemistry of natural compounds, 1991, vol. 27, no. 2, p. 212–215. DOI: 10.1007/BF00629762. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

Triterpene saponins Triterpene saponins (RF-00004338-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Triterpene saponins in leaves

Composition #10: Kemertelidze, E.P., Gvazava, L.N. Gitaloxin from Digitalis ciliata. chemistry of natural compounds, 1976, vol. 12, no. 6, p. 745. DOI: 10.1007/BF00564991. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Unspecified
Substance:

gitaloxin (RF-00003834-PAR)
Analytical method code:

Chromatographic tests (F015A)
Analytical method - additional description:

Chemical tests
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Gitaloxin (leaves)

Composition #11: Kemertelidze, E.P. Cardiac glycosides of Digitalis ciliata Trautv. - II. Acetyldigitoxin. chemistry of natural compounds, 1965, vol. 1, no. 6, p. 297–299. DOI: 10.1007/BF00568296. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extraction with 80% methanol, then dissolved in chloroform and passed through a column containing alumina
Substance:

acetyldigitoxin (RF-00003663-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Acetyldigitoxin (leaves)

Composition #12: Gvazava, L. N., Kikoladze, V. S. Steroidal glycosides from Digitalis ciliata leaves. chemistry of natural compounds, 2006, vol. 42, no. 5, p. 562–566. DOI: 10.1007/s10600-006-0214-3. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extraction with n-butanol, ethanol and acetone
Substance:

steroidal glycosides (RF-00011482-PAR)
Additional substance description:

furostane and 25R-spirostane
Analytical method code:

Chromatographic tests (F015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Steroidal glycosides (furostane and 25R-spirostane) (leaves)

Composition #13: Gvazava, L. N., Kukoladze, V. S. Sterohal glycoside-protodioscin from Digitalis ciliata. chemistry of natural compounds, 2006, vol. 42, no. 5, p. 614–615. DOI: 10.1007/s10600-006-0231-2. [DOI link]

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified
Substance:

protodioscin (RF-00004009-PAR)
Analytical method code:

Chromatographic tests (F015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

protodioscin

Composition #14: Kemertelidze, E. P., Skhirtladze, A. V., Ganzera, M. Steroidal and Triterpenoid Glycosides from Roots of Digitalis ciliata. chemistry of natural compounds, 2017, vol. 53, no. 3, p. 492–496. DOI: 10.1007/s10600-017-2029-9. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

80% methanol
Substance:

steroidal furostan saponins steroidal furostan saponins (RF-00004397-PAR)
Additional substance description:

ciliatasides A, B and C
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Steroidal furostan saponines (ciliatasides A, B and C) (roots)

Composition #15: Kemertelidze, E. P., Skhirtladze, A. V., Ganzera, M. Steroidal and Triterpenoid Glycosides from Roots of Digitalis ciliata. chemistry of natural compounds, 2017, vol. 53, no. 3, p. 492–496. DOI: 10.1007/s10600-017-2029-9. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

80% methanol
Substance:

cardiac glycosides (RF-00010823-PAR)
Additional substance description:

Digisilicide A, B and C
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Cardiac glycosides (digisilicide A, B and C) (roots)

Composition #16: KEMERTELIDZE, EP., GVAZAVA, LN., ALANIA, MD., KIKOLADZE, VS., TSITSISHVILI, VG. DIGITOSIDE, A NOVEL TRITERPENE GLYCOSIDE FROM DIGITALIS-CILIATA. journal of natural products, 1992, vol. 55, no. 2, p. 217–220. DOI: 10.1021/np50080a010. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70% methanol
Substance:

cardiac glycosides (RF-00010823-PAR)
Additional substance description:

Digitoside
Analytical method code:

Chromatographic tests (F015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Cardiac glycoside (digitoside) (leaves)