EFSA Compendium of Botanicals

Draft - work in progress

Cytisus scoparius (L.) Link // Sarothamnus scoparius (L.) W.D.J.Koch

Family:: Fabaceae
Approval state:: Approved
Number of articles retrieved:: 1058
Number of processed references:: 3

Compositions

#1: sparteine (broom tops) #2: Lupanine and its derivatives: (+)-13 alpha-hydroxylupanine, (+)-13 alpha-tigloyloxylupanine, etc. (seedlings) #3: oxosparteine (seedlings) #4: sparteine (seedlings) #5: Quinolizidine alkaloids alpha-Isosparteine, sparteine, 11,12-dehydrosparteine, tetrahydrorhom bifoline , 17-oxosparteine, angustifoline , alpha-isolupanine , 5,6-dehydrolupanine, lupanine, aphylline, 11,12- dehydrolupanine, 3ß-hydroxylupanine, multiflorine, 10,17-dioxosparteine, 17-oxolupanine, anagyrine, 13a-hydroxylupanine, 3ß,13a-dihydroxylupanine, 13alpha-angeloyloxylupanine and 13alpha-tigloyloxylupanine, aphylline and 10,17-dioxosparteine

Endpoint Studies

Genotoxicity

Composition #1: Gilfillan, F. A., Logan, Felipe P. The alkaloidal content of Oregon-grown Cytisus scoparius (Scotch broom). journal of the american pharmaceutical association (1912-1977), 1936, vol. 25, p. 505–6. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alcohol, sulfuric acid
Substance:

(-)-Sparteine (RF-00004059-PAR)
Analytical method code:

Unspecified (F598A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.863-1.026
Internal remarks:

sparteine (broom tops)

Composition #2: SAITO, K., SUZUKI, H., YAMASHITA, Y., MURAKOSHI, I. ISOLATION AND ENZYMATIC-SYNTHESIS OF AN ESTER ALKALOID, (-)-3-BETA-HYDROXY-13-ALPHA-TIGLOYLOXYLUPANINE, FROM CYTISUS-SCOPARIUS . phytochemistry, 1994, vol. 36, no. 2, p. 309–311. DOI: 10.1016/S0031-9422(00)97066-3. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

Lupanine (RF-00003910-PAR)
Additional substance description:

Lupanine and many its derivatives: (+)-13 alpha-hydroxylupanine, (+)-13 alpha-tigloyloxylupanine, etc.
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

co-HPLC, co-TLC and GC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Lupanine and its derivatives: (+)-13 alpha-hydroxylupanine, (+)-13 alpha-tigloyloxylupanine, etc. (seedlings)

Composition #3: SAITO, K., SUZUKI, H., YAMASHITA, Y., MURAKOSHI, I. ISOLATION AND ENZYMATIC-SYNTHESIS OF AN ESTER ALKALOID, (-)-3-BETA-HYDROXY-13-ALPHA-TIGLOYLOXYLUPANINE, FROM CYTISUS-SCOPARIUS . phytochemistry, 1994, vol. 36, no. 2, p. 309–311. DOI: 10.1016/S0031-9422(00)97066-3. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

(-)-Sparteine (RF-00004059-PAR)
Additional substance description:

Sparteine derivative: oxosparteine
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

co-HPLC, co-TLC and GC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

oxosparteine (seedlings)

Composition #4: SAITO, K., SUZUKI, H., YAMASHITA, Y., MURAKOSHI, I. ISOLATION AND ENZYMATIC-SYNTHESIS OF AN ESTER ALKALOID, (-)-3-BETA-HYDROXY-13-ALPHA-TIGLOYLOXYLUPANINE, FROM CYTISUS-SCOPARIUS . phytochemistry, 1994, vol. 36, no. 2, p. 309–311. DOI: 10.1016/S0031-9422(00)97066-3. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

(-)-Sparteine (RF-00004059-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

co-HPLC, co-TLC and GC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

sparteine (seedlings)

Composition #5: Gresser, G., Witte, L., Dedkov, VP., Czygan, FC. A survey of quinolizidine alkaloids and phenylethylamine tyramine in Cytisus scoparius (Leguminosae) from different origins . zeitschrift fur naturforschung c-a journal of biosciences, 1996, vol. 51, no. 11-12, p. 791–801. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

Quinolizidine alkaloids (RF-00004327-PAR)
Additional substance description:

alpha-Isosparteine, sparteine, 11,12-dehydrosparteine, tetrahydrorhom bifoline , 17-oxosparteine, angustifoline , alpha-isolupanine , 5,6-dehydrolupanine, lupanine, etc.
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Quinolizidine alkaloids alpha-Isosparteine, sparteine, 11,12-dehydrosparteine, tetrahydrorhom bifoline , 17-oxosparteine, angustifoline , alpha-isolupanine , 5,6-dehydrolupanine, lupanine, aphylline, 11,12- dehydrolupanine, 3ß-hydroxylupanine, multiflorine, 10,17-dioxosparteine, 17-oxolupanine, anagyrine, 13a-hydroxylupanine, 3ß,13a-dihydroxylupanine, 13alpha-angeloyloxylupanine and 13alpha-tigloyloxylupanine, aphylline and 10,17-dioxosparteine