EFSA Compendium of Botanicals

Draft - work in progress

Crinum asiaticum L (as plant) // Crinum asiaticum L.

Family:: Amaryllidaceae
Approval state:: Approved
Number of articles retrieved:: 202
Number of processed references:: 10

Endpoint Studies

Genotoxicity

Composition #1: Boit, Hans G., Dopke, Werner., Stender, Wolfgang. Amaryllidaceae Alkaloids. XX. Alkaloids from crinum, zephyranthes, leucojum, and clivia species. chemische berichte, 1957, vol. 90, p. 2203–6. .

Language:

English (en)
Plant part:

Bulb / clove (as part-nature) (A067A)
Additional extraction description:

Not specified
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

0.05% total alkaloids: of which 0.043% Lycorin, 0.002% haemanthamine, 0.001% crinidine, 0.001% crinamine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.05
Internal remarks:

Alkaloids in bulb

Composition #2: Ghosal, Shibnath., Kumar, Yatendra., Singh, Sushil K., Kumar, Anand. Chemical constituents of Amaryllidaceae. Part 21. Ungeremine and criasbetaine, two antitumor alkaloids from Crinum asiaticum. journal of chemical research, synopses, 1986, vol. (3), p. 112–13. .

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Additional extraction description:

Not specified
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

Ungeremine and criasbetaine
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Alkaloids (fruit)

Composition #3: Phan, Tong Son., Tran, Bach Duong., Phan, Minh Giang., Nguyen, Thim Minh., Nguyen, Quang Vu., Taylor, Walter C. Study on alkaloids from Crinum asiaticum L. (Amaryllidaceae) of Vietnam. tap chi hoa hoc, 2002, vol. 40, no. 2, p. 53–58. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Additional extraction description:

Not specified
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

hippadine, pratorimine, pratorinine, crinine, 1-O-acetyl-lycorine and lycorine
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Alkaloids

Composition #4: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

genistein (RF-00000297-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPLC-ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

59.2
Internal remarks:

Genistein

Composition #5: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

biochanin-A (RF-00004182-PAR)
Additional substance description:

Isoflavonoids: Biochanin A, Diadzin, Formononetin, Glycitein, Prunetin
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPLC-ELISA
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isoflavonoids

Composition #6: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

Isoformononetin (RF-00011018-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPLC-ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

32
Internal remarks:

Isoformononetin

Composition #7: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

Ononin (RF-00011023-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPLC-ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

47.9
Internal remarks:

Ononin

Composition #8: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

Sissotrin (RF-00011021-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPLC-ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

54.9
Internal remarks:

Sissotrin

Composition #9: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

daidzein (RF-00000061-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPLC-ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

39.4
Internal remarks:

Daidzein

Composition #10: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

genistin (RF-00003829-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPLC-ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

94.7
Internal remarks:

Genistin

Composition #11: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

Puerarin (RF-00011022-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

HPLC-ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

89.2
Internal remarks:

Puerarin

Composition #12: Davey, MW., Persiau, G., De Bruyn, A., Van Damme, S., Bauw, G., Van Montagu, M. Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography . analytical biochemistry, 1998, vol. 257, no. 1, p. 80–88. DOI: 10.1006/abio.1997.2544. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

sulfuric acid extraction
Substance:

lycorine (RF-00003913-PAR)
Additional substance description:

alkaloid
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Millimole/kilogram (G070A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.0
Internal remarks:

Lycorine (leaves)

Composition #13: Davey, MW., Persiau, G., De Bruyn, A., Van Damme, S., Bauw, G., Van Montagu, M. Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography . analytical biochemistry, 1998, vol. 257, no. 1, p. 80–88. DOI: 10.1006/abio.1997.2544. [DOI link]

Language:

English (en)
Plant part:

Stem / stalk (as part-nature) (A067S)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

sulfuric acid extraction
Substance:

lycorine (RF-00003913-PAR)
Additional substance description:

alkaloid
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Millimole/kilogram (G070A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.5
Internal remarks:

Lycorin (stem)

Composition #14: Davey, MW., Persiau, G., De Bruyn, A., Van Damme, S., Bauw, G., Van Montagu, M. Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography . analytical biochemistry, 1998, vol. 257, no. 1, p. 80–88. DOI: 10.1006/abio.1997.2544. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

sulfuric acid extraction
Substance:

lycorine (RF-00003913-PAR)
Additional substance description:

alkaloid
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Millimole/kilogram (G070A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

8.3
Internal remarks:

Lycorine (root)

Composition #15: Mustafa, NR., Rhee, IK., Verpoorte, R. Rapid method for determination of galanthamine in Amaryllidaceae plants using HPLC . journal of liquid chromatography & related technologies, 2003, vol. 26, no. 19, p. 3217–3233. DOI: 10.1081/JLC-120025519. [DOI link]

Language:

English (en)
Plant part:

Bulb / clove (as part-nature) (A067A)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acid–base extraction method or acid extraction method
Substance:

galanthamine (RF-00003399-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Galanthamine (bulb)

Composition #16: Zhang, Xi., Huang, Hao., Liang, Xu., Huang, Haiqiang., Dai, WeiXing., Shen, YunHeng., Yan, ShiKai., Zhang, WeiDong. Analysis of Amaryllidaceae alkaloids from Crinum by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry . rapid communications in mass spectrometry, 2009, vol. 23, no. 18, p. 2903–2916. DOI: 10.1002/rcm.4205. [DOI link]

Language:

English (en)
Plant part:

Bulb / clove (as part-nature) (A067A)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOh extract
Substance:

haemanthamine (RF-00003414-PAR)
Analytical method code:

LC-MS (F026A)
Analytical method - additional description:

LC-MS-ESI
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Haemanthamine (bulb)

Composition #17: Zhang, Xi., Huang, Hao., Liang, Xu., Huang, Haiqiang., Dai, WeiXing., Shen, YunHeng., Yan, ShiKai., Zhang, WeiDong. Analysis of Amaryllidaceae alkaloids from Crinum by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry . rapid communications in mass spectrometry, 2009, vol. 23, no. 18, p. 2903–2916. DOI: 10.1002/rcm.4205. [DOI link]

Language:

English (en)
Plant part:

Bulb / clove (as part-nature) (A067A)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOh extract
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

Tazettine-type alkaloids (Latifaliumin B and C) and Crinane-type alkaloids (11-OMethylcrinamine, Powelline, Hamayane and derivatives, Crinane-3a-ol, Buphanidrine)
Analytical method code:

LC-MS (F026A)
Analytical method - additional description:

LC-MS-ESI
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Alkaloids (bulbs)

Composition #18: Asmawi, M. Z., Arafat, O. M., Amirin, S., Eldeen, I. M. In vivo Antinociceptive Activity of Leaf Extract of Crinum asiaticum and Phytochemical Analysis of the Bioactive Fractions . international journal of pharmacology, 2011, vol. 7, no. 1, p. 125–129. DOI: 10.3923/ijp.2011.125.129. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform extract
Substance:

Alkaloids (RF-00004360-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

TLC
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Alkaloids (leaves)

Composition #19: Asmawi, M. Z., Arafat, O. M., Amirin, S., Eldeen, I. M. In vivo Antinociceptive Activity of Leaf Extract of Crinum asiaticum and Phytochemical Analysis of the Bioactive Fractions . international journal of pharmacology, 2011, vol. 7, no. 1, p. 125–129. DOI: 10.3923/ijp.2011.125.129. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform extract
Substance:

coumarins (RF-00004351-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

TLC
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Coumarins (leaves)

Composition #20: GHOSAL, S., SHANTHY, A., KUMAR, A., KUMAR, Y. CHEMICAL-CONSTITUENTS OF AMARYLLIDACEAE .18. PALMILYCORINE AND LYCORISIDE - ACYLOXY AND ACYLGLUCOSYLOXY ALKALOIDS FROM CRINUM-ASIATICUM . phytochemistry, 1985, vol. 24, no. 11, p. 2703–2706. DOI: 10.1016/S0031-9422(00)80698-6. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

crinasiatine, hippadine, lycoriside, palmilycorine
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

HPLC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Alkaloids (fruit)

Composition #21: GHOSAL, S., SHANTHY, A., KUMAR, A., KUMAR, Y. CHEMICAL-CONSTITUENTS OF AMARYLLIDACEAE .18. PALMILYCORINE AND LYCORISIDE - ACYLOXY AND ACYLGLUCOSYLOXY ALKALOIDS FROM CRINUM-ASIATICUM . phytochemistry, 1985, vol. 24, no. 11, p. 2703–2706. DOI: 10.1016/S0031-9422(00)80698-6. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

ambelline (RF-00004141-PAR)
Additional substance description:

ambelline and derivatives
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

HPLC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Ambelline and derivatives (fruit)

Composition #22: GHOSAL, S., SHANTHY, A., KUMAR, A., KUMAR, Y. CHEMICAL-CONSTITUENTS OF AMARYLLIDACEAE .18. PALMILYCORINE AND LYCORISIDE - ACYLOXY AND ACYLGLUCOSYLOXY ALKALOIDS FROM CRINUM-ASIATICUM . phytochemistry, 1985, vol. 24, no. 11, p. 2703–2706. DOI: 10.1016/S0031-9422(00)80698-6. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

crinamine (RF-00004228-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

HPLC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Crinamine (fruit)

Composition #23: GHOSAL, S., SHANTHY, A., KUMAR, A., KUMAR, Y. CHEMICAL-CONSTITUENTS OF AMARYLLIDACEAE .18. PALMILYCORINE AND LYCORISIDE - ACYLOXY AND ACYLGLUCOSYLOXY ALKALOIDS FROM CRINUM-ASIATICUM . phytochemistry, 1985, vol. 24, no. 11, p. 2703–2706. DOI: 10.1016/S0031-9422(00)80698-6. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH extraction
Substance:

lycorine (RF-00003913-PAR)
Additional substance description:

lycorine- 1 -O-beta-D-glucoside
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

HPLC-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Lycorine (fruit)

Composition #24: BEUTNER, D., FRAHM, AW. NEW ALKALOIDS FROM CRINUM-ASIATICUM. planta medica, 1986, no. 6, p. 523–523. .

Language:

English (en)
Plant part:

Bulb / clove (as part-nature) (A067A)
Additional extraction description:

Extracts from bubs and roots - method of extraction not specified
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

(-) crinane, (+) crinane and flexinine
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Alkaloids (leaves and bulbs)