EFSA Compendium of Botanicals

Draft - work in progress

Coleus forskohlii (Willd.) Briq. // Plectranthus barbatus Andr.

Family:: Lamiaceae
Approval state:: Approved
Number of articles retrieved:: 833
Number of processed references:: 6

Compositions

#1: Forskolin from stems #2: Forskolin from roots #3: forskolin and derivatives in aerial parts #4: Cardiac glycosides #5: forskolin from roots #6: alkaloids analysis of various extracts of Coleus forskohlii leaves and roots #7: coumarins analysis of various extracts of Coleus forskohlii leaves and roots #8: saponins analysis of various extracts of Coleus forskohlii leaves and roots #9: labdane diterpenes in roots

Endpoint Studies

#1: hepatotoxicity with diet containing 0.5% forskolin

Genotoxicity

Composition #1: Schaneberg, BT., Khan, IA. Quantitative analysis of forskolin in Coleus forskohlii (Lamiaceae) by reversed-phase liquid chromatography . journal of aoac international, 2003, vol. 86, no. 3, p. 467–470. .

Language:

English (en)
Plant part:

Stem / stalk (as part-nature) (A067S)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetonitrile + sonication
Substance:

forskolin (RF-00004295-PAR)
Analytical method code:

LC-MS (F026A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.03
Internal remarks:

Forskolin from stems

Composition #2: Schaneberg, BT., Khan, IA. Quantitative analysis of forskolin in Coleus forskohlii (Lamiaceae) by reversed-phase liquid chromatography . journal of aoac international, 2003, vol. 86, no. 3, p. 467–470. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetonitrile + sonication
Substance:

forskolin (RF-00004295-PAR)
Analytical method code:

LC-MS (F026A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.3
Internal remarks:

Forskolin from roots

Composition #3: Bodiwala, Hardik S., Sabde, Sudeep., Mitra, Debashis., Bhutani, Kamlesh Kumar., Singh, Inder Pal. Anti-MV Diterpenes from Coleus forskohlii. natural product communications, 2009, vol. 4, no. 9, p. 1173–1175. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Chloroform
Substance:

forskolin (RF-00004295-PAR)
Additional substance description:

forskolin and derivatives
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

forskolin and derivatives in aerial parts

Composition #4: Khatun, Selima., Cakilcioglu, Ugur., Chatterjee, Narayan Chandra. Phytochemical constituents vis-a-vis histochemical localization of forskolin in a medicinal plant Coleus forskohlii Briq. . journal of medicinal plants research, 2011, vol. 5, no. 5, p. 711–718. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Distilled water
Substance:

cardiac glycosides (RF-00010823-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Cardiac glycosides

Composition #5: Virgona, Nantiga., Taki, Yuko., Yamada, Shizuo., Umegaki, Keizo. Dietary Coleus forskohlii extract generates dose-related hepatotoxicity in mice . journal of applied toxicology, 2013, vol. 33, no. 9, p. 924–932. DOI: 10.1002/jat.2770. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Super-critical gas extraction (A0BZQ)
Additional extraction description:

CO2 gas
Substance:

forskolin (RF-00004295-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

20-30
Internal remarks:

forskolin from roots

Composition #6: Takshak, Swabha., Agrawal, S. B. The role of supplemental ultraviolet-B radiation in altering the metabolite profile, essential oil content and composition, and free radical scavenging activities of Coleus forskohlii, an indigenous medicinal plant . environmental science and pollution research, 2016, vol. 23, no. 8, p. 7324–7337. DOI: 10.1007/s11356-015-5965-6. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

different solvents were used (100 ml each; methanol, ethanol, petroleum ether, acetone, chloroform, and water)
Substance:

Alkaloids (RF-00004360-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

alkaloids analysis of various extracts of Coleus forskohlii leaves and roots

Composition #7: Takshak, Swabha., Agrawal, S. B. The role of supplemental ultraviolet-B radiation in altering the metabolite profile, essential oil content and composition, and free radical scavenging activities of Coleus forskohlii, an indigenous medicinal plant . environmental science and pollution research, 2016, vol. 23, no. 8, p. 7324–7337. DOI: 10.1007/s11356-015-5965-6. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

different solvents were used (100 ml each; methanol, ethanol, petroleum ether, acetone, and water)
Substance:

coumarins (RF-00004351-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

coumarins analysis of various extracts of Coleus forskohlii leaves and roots

Composition #8: Takshak, Swabha., Agrawal, S. B. The role of supplemental ultraviolet-B radiation in altering the metabolite profile, essential oil content and composition, and free radical scavenging activities of Coleus forskohlii, an indigenous medicinal plant . environmental science and pollution research, 2016, vol. 23, no. 8, p. 7324–7337. DOI: 10.1007/s11356-015-5965-6. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

different solvents were used (100 ml each; methanol, ethanol, petroleum ether, acetone, and water)
Substance:

Saponins (RF-00004333-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

saponins analysis of various extracts of Coleus forskohlii leaves and roots

Composition #9: ROY, R., MISHRA, A., VARMA, N., TANDON, JS., SAUX, M., CARPY, A. MINOR DITERPENES FROM COLEUS-FORSKOHLII. phytochemistry, 1993, vol. 34, no. 6, p. 1577–1580. DOI: 10.1016/S0031-9422(00)90848-3. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

1,2-dichloroethane, methanol
Substance:

labdane diterpenes (RF-00004392-PAR)
Additional substance description:

1,9-dideoxy coleonol-B, 1-acetoxy coleosol
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

1D H-NMR, IR and MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

labdane diterpenes in roots

Endpoint study #1: Virgona, Nantiga., Taki, Yuko., Yamada, Shizuo., Umegaki, Keizo. Dietary Coleus forskohlii extract generates dose-related hepatotoxicity in mice . journal of applied toxicology, 2013, vol. 33, no. 9, p. 924–932. DOI: 10.1002/jat.2770. [DOI link]

Study relevance:

Human health
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Preparation/Extraction method:

Super-critical gas extraction (A0BZQ)
Additional plant/substance description:

standardized CFE containing 10.88% forskolin w/w
Type of toxicological test:

short-term toxicity (CHD017TT)
Tested organism:

House mouse (as animal) (A0CNT)
Strain of test organism:

ICR (CHD044ST)
Sex:

Male
Route of exposure:

ORAL: FEED (OECD2230PL)
Exposure duration:

3.0
Unit of exposure duration:

Week (G205A)
Number of organisms dosed:

6
Control groups used:

yes
Endpoint measured:

Dose level (CHD014EP)
Concentration unit:

Milligram/kilogram (G061A)
Effect concentration:

500
Critical effect:

HEPATOTOXICITY (TOX02A)
Target tissue:

digestive: Liver (TT023A)
Effect description:

hepatotoxicity: Hepatocyte single cell necrosis, Hepatocyte hypertrophy, fatty change with diet containing 0.5% forskolin
Effect or parameter linked to the endpoint:

histopathology non neoplastic (CHD025BE)
Internal remarks:

hepatotoxicity with diet containing 0.5% forskolin