EFSA Compendium of Botanicals

Draft - work in progress

Clivia miniata (Lindl.) Bosse

Family:: Amaryllidaceae
Approval state:: Approved
Number of articles retrieved:: 267
Number of processed references:: 4

Endpoint Studies

#1: Neurotoxicity, unspecified animal species.

Genotoxicity

Composition #1: Briggs, Carol K., Highet, Patricia F., Highet, R. J., Wildman, W. C. Alkaloids of the Amaryllidaceae. VII. Alkaloids containing the hemiacetal or lactone group. journal of the american chemical society, 1956, vol. 78, p. 2899–2904. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Additional extraction description:

unspecified
Substance:

lycorine (RF-00003913-PAR)
Analytical method code:

Unspecified (F598A)
Result unit:

Percent (G138A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.53
Internal remarks:

Lycorine (rhizomes)

Composition #2: Briggs, Carol K., Highet, Patricia F., Highet, R. J., Wildman, W. C. Alkaloids of the Amaryllidaceae. VII. Alkaloids containing the hemiacetal or lactone group. journal of the american chemical society, 1956, vol. 78, p. 2899–2904. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Additional extraction description:

unspecified
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

Clivonine
Analytical method code:

Unspecified (F598A)
Result unit:

Percent (G138A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.07
Internal remarks:

Clivonine (rhizomes)

Composition #3: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

Sissotrin (RF-00011021-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

43.4
Internal remarks:

Sissotrin (leaves)

Composition #4: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

Ononin (RF-00011023-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

59.0-80.1
Internal remarks:

Ononin (leaves)

Composition #5: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

daidzein (RF-00000061-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=21.8
Internal remarks:

Daidzein (leaves)

Composition #6: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

genistein (RF-00000297-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

23.8-30.5
Internal remarks:

Genistein (leaves)

Composition #7: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

glycitin (RF-00003847-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=122.5
Internal remarks:

Glycitin (leaves)

Composition #8: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

genistin (RF-00003829-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

399.7
Internal remarks:

Genistin (roots)

Composition #9: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

Puerarin (RF-00011022-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

27.2
Internal remarks:

Puerarin (roots)

Composition #10: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

formononetin (RF-00000291-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

23.4
Internal remarks:

Formononetin (roots)

Composition #11: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

daidzin (RF-00003763-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Value (concentration, content):

traces
Internal remarks:

Daidzin (leaves)

Composition #12: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

daidzein (RF-00000061-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Value (concentration, content):

traces
Internal remarks:

Daidzein (roots)

Composition #13: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

biochanin-A (RF-00004182-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=9.2
Internal remarks:

Biochanin A (leaves)

Composition #14: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

glycitin (RF-00003847-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Value (concentration, content):

traces
Internal remarks:

Glycitin (roots)

Composition #15: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

formononetin (RF-00000291-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

8.7-31.0
Internal remarks:

Formononetin (leaves)

Composition #16: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

genistein (RF-00000297-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Value (concentration, content):

traces
Internal remarks:

Genistein (roots)

Composition #17: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

genistin (RF-00003829-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=117.5
Internal remarks:

Genistin (leaves)

Composition #18: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

Sissotrin (RF-00011021-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Value (concentration, content):

traces
Internal remarks:

Sissotrin (roots)

Composition #19: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

biochanin-A (RF-00004182-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

Identity verified by ELISA
Value (concentration, content):

traces
Internal remarks:

Biochanin A (roots)

Composition #20: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

Puerarin (RF-00011022-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

18.7
Internal remarks:

Puerarin (leaves)

Composition #21: Miksatkova, Petra., Lankova, Petra., Huml, Lukas., Lapcik, Oldrich. Isoflavonoids in the Amaryllidaceae family. natural product research, 2014, vol. 28, no. 10, p. 690–697. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

70 % ethanol
Substance:

isoflavones (RF-00004359-PAR)
Additional substance description:

tectoridin
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/gram (G076A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

324.8
Internal remarks:

Isoflavone tectoridin (roots)

Composition #22: Nair, Jerald J., van Staden, Johannes. Pharmacological and toxicological insights to the South African Amaryllidaceae . food and chemical toxicology, 2013, vol. 62, p. 262–275. DOI: 10.1016/j.fct.2013.08.042. [DOI link]

Language:

English (en)
Plant part:

Unspecified (A07XD)
Additional extraction description:

unspecified
Substance:

indole alkaloids (RF-00004503-PAR)
Additional substance description:

Haemanthamine, caranine, lycorine and hippeastrine
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

indole alkaloids

Composition #23: VANDAMME, EJM., GOLDSTEIN, IJ., PEUMANS, WJ. A COMPARATIVE-STUDY OF MANNOSE-BINDING LECTINS FROM THE AMARYLLIDACEAE AND ALLIACEAE . phytochemistry, 1991, vol. 30, no. 2, p. 509–514. DOI: 10.1016/0031-9422(91)83716-X. [DOI link]

Language:

English (en)
Plant part:

Bulb / clove (as part-nature) (A067A)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

1M (NH4)2SO4; isolated by gel permeation
Substance:

Lectins (RF-00000439-ORG)
Additional substance description:

Mannose-binding polypeptides Mr = 13,000 and 14,000
Analytical method code:

Gel electrophoresis (F076A)
Analytical method - additional description:

SDS-PAGE
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Lectins

Endpoint study #1: Nair, Jerald J., van Staden, Johannes. Pharmacological and toxicological insights to the South African Amaryllidaceae . food and chemical toxicology, 2013, vol. 62, p. 262–275. DOI: 10.1016/j.fct.2013.08.042. [DOI link]

Study relevance:

Human health
Substance:

indole alkaloids (RF-00004503-PAR)
Additional plant/substance description:

Haemanthamine, caranine, lycorine and hippeastrine
Type of toxicological test:

not reported (CHD015TT)
Limit test:

No data
Sex:

No data
Control groups used:

no data
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

NEUROTOXICITY (TOX04A)
Target tissue:

neurologic: Central Nervous System (TT035A)
Effect description:

analgesia, paralysis, respiratory paralysis
Effect or parameter linked to the endpoint:

mobility (CHD011BE)
Internal remarks:

Neurotoxicity, unspecified animal species.