EFSA Compendium of Botanicals

Draft - work in progress

Chelidonium majus L. // Chelidonium umbelliferum Stokes

Family:: Papaveraceae
Approval state:: Approved
Number of articles retrieved:: 1534
Number of processed references:: 7

Compositions

Endpoint Studies

Genotoxicity

#1: DNA damage in mouse spleen and L1210 cells

Composition #1: Migas, Piotr., Heyka, Magdalena., Poblocka-Olech, Loretta., Krauze-Baranowska, Miroslawa. BMD-TLC - the useful technique for quantitative analysis of chelidonine, chelerythrine and berberine in herbal drugs. journal of planar chromatography--modern tlc, 2012, vol. 25, no. 5, p. 439–444. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid (12%)
Substance:

chelidonine (RF-00004205-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.332
Internal remarks:

chelidonine (aerial parts)

Composition #2: Migas, Piotr., Heyka, Magdalena., Poblocka-Olech, Loretta., Krauze-Baranowska, Miroslawa. BMD-TLC - the useful technique for quantitative analysis of chelidonine, chelerythrine and berberine in herbal drugs. journal of planar chromatography--modern tlc, 2012, vol. 25, no. 5, p. 439–444. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid (12%)
Substance:

benzophenanthridine alkaloids (RF-00010802-PAR)
Additional substance description:

chelerithrine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.078
Internal remarks:

chelerithrine (aerial parts)

Composition #3: Migas, Piotr., Heyka, Magdalena., Poblocka-Olech, Loretta., Krauze-Baranowska, Miroslawa. BMD-TLC - the useful technique for quantitative analysis of chelidonine, chelerythrine and berberine in herbal drugs. journal of planar chromatography--modern tlc, 2012, vol. 25, no. 5, p. 439–444. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid (12%)
Substance:

berberine (RF-00003706-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.058
Internal remarks:

Berberine (aerial parts)

Composition #4: Migas, Piotr., Heyka, Magdalena., Poblocka-Olech, Loretta., Krauze-Baranowska, Miroslawa. BMD-TLC - the useful technique for quantitative analysis of chelidonine, chelerythrine and berberine in herbal drugs. journal of planar chromatography--modern tlc, 2012, vol. 25, no. 5, p. 439–444. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid (12%)
Substance:

berberine (RF-00003706-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.055
Internal remarks:

Berberine (root)

Composition #5: Migas, Piotr., Heyka, Magdalena., Poblocka-Olech, Loretta., Krauze-Baranowska, Miroslawa. BMD-TLC - the useful technique for quantitative analysis of chelidonine, chelerythrine and berberine in herbal drugs. journal of planar chromatography--modern tlc, 2012, vol. 25, no. 5, p. 439–444. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid (12%)
Substance:

chelidonine (RF-00004205-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.349
Internal remarks:

chelidonine (root)

Composition #6: Migas, Piotr., Heyka, Magdalena., Poblocka-Olech, Loretta., Krauze-Baranowska, Miroslawa. BMD-TLC - the useful technique for quantitative analysis of chelidonine, chelerythrine and berberine in herbal drugs. journal of planar chromatography--modern tlc, 2012, vol. 25, no. 5, p. 439–444. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid (12%)
Substance:

benzophenanthridine alkaloids (RF-00010802-PAR)
Additional substance description:

chelerithrine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.082
Internal remarks:

chelerithrine (root)

Composition #7: Kursinszki, L., Sarkoezi, A., Kery, A., Szoeke, E. Improved RP-HPLC method for analysis of isoquinoline alkaloids in extracts of Chelidonium majus . chromatographia, 2006, vol. 63, p. S131–S135. DOI: 10.1365/s10337-006-0808-8. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

protopine (RF-00004010-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.043
Internal remarks:

protopine (aerial parts)

Composition #8: Kursinszki, L., Sarkoezi, A., Kery, A., Szoeke, E. Improved RP-HPLC method for analysis of isoquinoline alkaloids in extracts of Chelidonium majus . chromatographia, 2006, vol. 63, p. S131–S135. DOI: 10.1365/s10337-006-0808-8. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

coptisine (RF-00004215-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.361
Internal remarks:

coptisine (aerial parts)

Composition #9: Kursinszki, L., Sarkoezi, A., Kery, A., Szoeke, E. Improved RP-HPLC method for analysis of isoquinoline alkaloids in extracts of Chelidonium majus . chromatographia, 2006, vol. 63, p. S131–S135. DOI: 10.1365/s10337-006-0808-8. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

berberine (RF-00003706-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.013
Internal remarks:

berberine (aerial parts)

Composition #10: Kursinszki, L., Sarkoezi, A., Kery, A., Szoeke, E. Improved RP-HPLC method for analysis of isoquinoline alkaloids in extracts of Chelidonium majus . chromatographia, 2006, vol. 63, p. S131–S135. DOI: 10.1365/s10337-006-0808-8. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

sanguinarine (RF-00004040-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.041
Internal remarks:

sanguinarine (aerial parts)

Composition #11: Kursinszki, L., Sarkoezi, A., Kery, A., Szoeke, E. Improved RP-HPLC method for analysis of isoquinoline alkaloids in extracts of Chelidonium majus . chromatographia, 2006, vol. 63, p. S131–S135. DOI: 10.1365/s10337-006-0808-8. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

chelidonine (RF-00004205-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.027
Internal remarks:

chelidonine (aerial parts)

Composition #12: Kaminskyy, Vitaliy., Lin, Kah-Wai., Filyak, Yevhen., Stoika, Rostyslav. Differential effect of sanguinarine, chelerythrine and chelidonine on DNA damage and cell viability in primary mouse spleen cells and mouse leukemic cells . cell biology international, 2008, vol. 32, no. 2, p. 271–277. DOI: 10.1016/j.cellbi.2007.09.004. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Additional extraction description:

unspecified
Substance:

sanguinarine (RF-00004040-PAR)
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Sanguinarine (roots)

Composition #13: Kaminskyy, Vitaliy., Lin, Kah-Wai., Filyak, Yevhen., Stoika, Rostyslav. Differential effect of sanguinarine, chelerythrine and chelidonine on DNA damage and cell viability in primary mouse spleen cells and mouse leukemic cells . cell biology international, 2008, vol. 32, no. 2, p. 271–277. DOI: 10.1016/j.cellbi.2007.09.004. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Additional extraction description:

unspecified
Substance:

chelerythrine (RF-00004204-PAR)
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Chelerythrine (roots)

Composition #14: Cahlikova, Lucie., Opletal, Lubomir., Kurfurst, Milan., Macakova, Katerina., Kulhankova, Andrea., Host'alkova, Anna. Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Chelidonium majus (Papaveraceae) . natural product communications, 2010, vol. 5, no. 11, p. 1751–1754. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

chelerythrine (RF-00004204-PAR)
Additional substance description:

chelerythrine derivative: 6-ethoxydihydrochelerythrine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Column chromatography and preparative TLC
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

chelerythrine derivative: 6-ethoxydihydrochelerythrine (aerial and roots)

Composition #15: Cahlikova, Lucie., Opletal, Lubomir., Kurfurst, Milan., Macakova, Katerina., Kulhankova, Andrea., Host'alkova, Anna. Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Chelidonium majus (Papaveraceae) . natural product communications, 2010, vol. 5, no. 11, p. 1751–1754. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

chelidonine (RF-00004205-PAR)
Additional substance description:

chelidonine derivative: homochelidonine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Column chromatography and preparative TLC
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

chelidonine derivative: homochelidonine (aerial and roots)

Composition #16: Cahlikova, Lucie., Opletal, Lubomir., Kurfurst, Milan., Macakova, Katerina., Kulhankova, Andrea., Host'alkova, Anna. Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Chelidonium majus (Papaveraceae) . natural product communications, 2010, vol. 5, no. 11, p. 1751–1754. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

dihydrosanguinarine (RF-00003780-PAR)
Additional substance description:

dihydrosanguinarine derivative: 6-ethoxydihydrosanguinarine
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Column chromatography and preparative TLC
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

dihydrosanguinarine derivative: 6-ethoxydihydrosanguinarine (aerial and roots)

Composition #17: Kulp, Maria., Bragina, Olga., Kogerman, Priit., Kaljurand, Mihkel. Capillary electrophoresis with led-induced native fluorescence detection for determination of isoquinoline alkaloids and their cytotoxicity in extracts of Chelidonium majus L . journal of chromatography a, 2011, vol. 1218, no. 31, p. 5298–5304. DOI: 10.1016/j.chroma.2011.06.016. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

sanguinarine (RF-00004040-PAR)
Analytical method code:

Capillary (Zone) Electrophoresis (CE or CZE) (F071A)
Analytical method - additional description:

CE-LEDIF, HPLC-UV
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.041-0.055
Internal remarks:

Sanguinarine (aerial)

Composition #18: Kulp, Maria., Bragina, Olga., Kogerman, Priit., Kaljurand, Mihkel. Capillary electrophoresis with led-induced native fluorescence detection for determination of isoquinoline alkaloids and their cytotoxicity in extracts of Chelidonium majus L . journal of chromatography a, 2011, vol. 1218, no. 31, p. 5298–5304. DOI: 10.1016/j.chroma.2011.06.016. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

allocryptopine (RF-00004136-PAR)
Analytical method code:

Capillary (Zone) Electrophoresis (CE or CZE) (F071A)
Analytical method - additional description:

CE-LEDIF
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.028
Internal remarks:

Allocryptopine (aerial)

Composition #19: Kulp, Maria., Bragina, Olga., Kogerman, Priit., Kaljurand, Mihkel. Capillary electrophoresis with led-induced native fluorescence detection for determination of isoquinoline alkaloids and their cytotoxicity in extracts of Chelidonium majus L . journal of chromatography a, 2011, vol. 1218, no. 31, p. 5298–5304. DOI: 10.1016/j.chroma.2011.06.016. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

chelerythrine (RF-00004204-PAR)
Analytical method code:

Capillary (Zone) Electrophoresis (CE or CZE) (F071A)
Analytical method - additional description:

CE-LEDIF
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.040
Internal remarks:

Chelerythrine (aerial)

Composition #20: Kulp, Maria., Bragina, Olga., Kogerman, Priit., Kaljurand, Mihkel. Capillary electrophoresis with led-induced native fluorescence detection for determination of isoquinoline alkaloids and their cytotoxicity in extracts of Chelidonium majus L . journal of chromatography a, 2011, vol. 1218, no. 31, p. 5298–5304. DOI: 10.1016/j.chroma.2011.06.016. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

stylopine (RF-00003592-PAR)
Analytical method code:

Capillary (Zone) Electrophoresis (CE or CZE) (F071A)
Analytical method - additional description:

CE-LEDIF
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.038
Internal remarks:

Stylopine (aerial)

Composition #21: Kulp, Maria., Bragina, Olga., Kogerman, Priit., Kaljurand, Mihkel. Capillary electrophoresis with led-induced native fluorescence detection for determination of isoquinoline alkaloids and their cytotoxicity in extracts of Chelidonium majus L . journal of chromatography a, 2011, vol. 1218, no. 31, p. 5298–5304. DOI: 10.1016/j.chroma.2011.06.016. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

chelidonine (RF-00004205-PAR)
Analytical method code:

Capillary (Zone) Electrophoresis (CE or CZE) (F071A)
Analytical method - additional description:

CE-LEDIF, HPLC-UV
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.025-0.027
Internal remarks:

Chelidonine (aerial)

Composition #22: Kulp, Maria., Bragina, Olga., Kogerman, Priit., Kaljurand, Mihkel. Capillary electrophoresis with led-induced native fluorescence detection for determination of isoquinoline alkaloids and their cytotoxicity in extracts of Chelidonium majus L . journal of chromatography a, 2011, vol. 1218, no. 31, p. 5298–5304. DOI: 10.1016/j.chroma.2011.06.016. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

protopine (RF-00004010-PAR)
Analytical method code:

Capillary (Zone) Electrophoresis (CE or CZE) (F071A)
Analytical method - additional description:

CE-LEDIF, HPLC-UV
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.043-0.049
Internal remarks:

protopine (aerial)

Composition #23: Kulp, Maria., Bragina, Olga., Kogerman, Priit., Kaljurand, Mihkel. Capillary electrophoresis with led-induced native fluorescence detection for determination of isoquinoline alkaloids and their cytotoxicity in extracts of Chelidonium majus L . journal of chromatography a, 2011, vol. 1218, no. 31, p. 5298–5304. DOI: 10.1016/j.chroma.2011.06.016. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, 0.05 M HCl
Substance:

coptisine (RF-00004215-PAR)
Analytical method code:

Capillary (Zone) Electrophoresis (CE or CZE) (F071A)
Analytical method - additional description:

CE-LEDIF, HPLC-UV
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.361-0.763
Internal remarks:

coptisine (aerial)

Composition #24: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

chelerythrine (RF-00004204-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.143-3.906
Internal remarks:

Chelerythrine (aerial parts)

Composition #25: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

berberine (RF-00003706-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

25.545-30.231
Internal remarks:

Berberine (aerial parts)

Composition #26: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

coptisine (RF-00004215-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

746.901-819.745
Internal remarks:

Coptisine (roots)

Composition #27: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

chelidonine (RF-00004205-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

382.439-1780.667
Internal remarks:

Chelidonine (roots)

Composition #28: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

protopine (RF-00004010-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

72.036-368.95
Internal remarks:

Protopine (roots)

Composition #29: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

sanguinarine (RF-00004040-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

15.836-30.834
Internal remarks:

Sanguinarine (roots)

Composition #30: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

berberine (RF-00003706-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

8.866-60.098
Internal remarks:

Berberine (roots)

Composition #31: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

chelerythrine (RF-00004204-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

10.361-30.74
Internal remarks:

Chelerythrine (roots)

Composition #32: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

chelidonine (RF-00004205-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

115.929-187.233
Internal remarks:

Chelidonine (aerial parts)

Composition #33: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

coptisine (RF-00004215-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

287.474-1027.096
Internal remarks:

Coptisine (aerial parts)

Composition #34: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

protopine (RF-00004010-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

125.385-241.725
Internal remarks:

Protopine (aerial parts)

Composition #35: Bogucka-Kocka, Anna., Zalewski, Daniel. Qualitative and Quantitative Determination of Main Alkaloids of Chelidonium majus L. Using Thin-Layer Chromatographic-Densitometric Method . acta chromatographica, 2017, vol. 29, no. 3, p. 385–397. DOI: 10.1556/1326.2017.29.3.09. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetic acid
Substance:

sanguinarine (RF-00004040-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

Thin-Layer Chromatography
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

4.188-4.418
Internal remarks:

Sanguinarine (aerial parts)

Composition #36: NIU, CQ., HE, LY. DETERMINATION OF ISOQUINOLINE ALKALOIDS IN CHELIDONIUM-MAJUS L BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY . journal of chromatography, 1991, vol. 542, no. 1, p. 193–199. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform and ethanol (1:1)
Substance:

chelidonine (RF-00004205-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.503-1.020
Internal remarks:

Chelidonine (SUBTERRANEAN plant part)

Composition #37: NIU, CQ., HE, LY. DETERMINATION OF ISOQUINOLINE ALKALOIDS IN CHELIDONIUM-MAJUS L BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY . journal of chromatography, 1991, vol. 542, no. 1, p. 193–199. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform and ethanol (1:1)
Substance:

coptisine (RF-00004215-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.155-0.578
Internal remarks:

Coptisine (SUBTERRANEAN plant part)

Composition #38: NIU, CQ., HE, LY. DETERMINATION OF ISOQUINOLINE ALKALOIDS IN CHELIDONIUM-MAJUS L BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY . journal of chromatography, 1991, vol. 542, no. 1, p. 193–199. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform and ethanol (1:1)
Substance:

dihydrosanguinarine (RF-00003780-PAR)
Additional substance description:

Dihydrosanguinarine and derivative: 6-Methoxydihydrosanguinarine
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.019-0.151
Internal remarks:

Dihydrosanguinarine and derivative: 6-Methoxydihydrosanguinarine (SUBTERRANEAN plant part)

Composition #39: NIU, CQ., HE, LY. DETERMINATION OF ISOQUINOLINE ALKALOIDS IN CHELIDONIUM-MAJUS L BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY . journal of chromatography, 1991, vol. 542, no. 1, p. 193–199. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform and ethanol (1:1)
Substance:

protopine (RF-00004010-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.020-0.114
Internal remarks:

Protopine (SUBTERRANEAN plant part)

Composition #40: NIU, CQ., HE, LY. DETERMINATION OF ISOQUINOLINE ALKALOIDS IN CHELIDONIUM-MAJUS L BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY . journal of chromatography, 1991, vol. 542, no. 1, p. 193–199. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform and ethanol (1:1)
Substance:

berberine (RF-00003706-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.034-0.038
Internal remarks:

Berberine (SUBTERRANEAN plant part)

Composition #41: NIU, CQ., HE, LY. DETERMINATION OF ISOQUINOLINE ALKALOIDS IN CHELIDONIUM-MAJUS L BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY . journal of chromatography, 1991, vol. 542, no. 1, p. 193–199. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform and ethanol (1:1)
Substance:

Tetrahydropseudocoptisine (RF-00011427-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.064
Internal remarks:

Tetrahydrocoptisine (SUBTERRANEAN plant part)

Composition #42: NIU, CQ., HE, LY. DETERMINATION OF ISOQUINOLINE ALKALOIDS IN CHELIDONIUM-MAJUS L BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY . journal of chromatography, 1991, vol. 542, no. 1, p. 193–199. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

chloroform and ethanol (1:1)
Substance:

chelerythrine (RF-00004204-PAR)
Additional substance description:

chelerythrine derivative: 6-Methoxydihydrochelerythrine
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.081-0.198
Internal remarks:

chelerythrine derivative: 6-Methoxydihydrochelerythrine (SUBTERRANEAN plant part)

Genotox #1: Kaminskyy, Vitaliy., Lin, Kah-Wai., Filyak, Yevhen., Stoika, Rostyslav. Differential effect of sanguinarine, chelerythrine and chelidonine on DNA damage and cell viability in primary mouse spleen cells and mouse leukemic cells . cell biology international, 2008, vol. 32, no. 2, p. 271–277. DOI: 10.1016/j.cellbi.2007.09.004. [DOI link]

Study category:

Genotoxicity
Type of toxicological test:

single cell gel/comet assay in mammalian cells for detection of DNA damage (CHD040TT)
Method type:

in vitro
Tested organism:

House mouse (as animal) (A0CNT)
Strain of test organism:

spleen cells (CHD107ST)
Control groups used:

yes
Endpoint measured:

DNA damage and/or repair
Is genotoxic:

Positive
Remarks on genotox study:

Intensive DNA damage at concentration >1-8 μg/ml in mouse spleen and L1210 cells
Internal remarks:

DNA damage in mouse spleen and L1210 cells
Internal ID:

WOS:000260586300014