EFSA Compendium of Botanicals

Draft - work in progress

Calystegia sepium (L.) R.Br.

Family:: Convolvulaceae
Approval state:: Approved
Number of articles retrieved:: 326
Number of processed references:: 5

Compositions

#1: calystegines A3 from root culture #2: calystegines B2 (0.51 microgram/spot ) and B1 (0.39) from root culture #3: Four-five days after germination, seedlings of Calystegia sepium showed the highest concentration of calystegines (5.7 mg/g dry mass).In the plants grown up to 19 cm in the climate chamber after 1 week, calistegins in roots were in the same range as in seedlings, while in the aerial parts calistegins were less concentrated #4: In 3 weeks , old plants young leaves were the tissue with the highest calystegin levels ( 2.3 mg/g dry mass). Calystegins A3 and B2 were the major components with a rather constant ratio (1:2-3) in all tissues , except flowers which contained similar concentrations of these two caystegins and of calystegin B1. Calystegins A5 and B1 were detected in most samples, but could not be quantified. Other calystegins cannot be excluded, but 2,7-dihydrixynortropane was not found in the aerial part. #5: lectins ( Calystegia sepium agglutinin - Calsepa ) #6: cuscohygrine in roots #7: Calystegines are a group of polyhydroxy nortropane alkaloids that have been isolated from Calystegia sepium

Endpoint Studies

Genotoxicity

Composition #1: Scholl, Y., Asano, N., Drager, B. Automated multiple development thin layer chromatography for calystegines and their biosynthetic precursors . journal of chromatography a, 2001, vol. 928, no. 2, p. 217–224. DOI: 10.1016/S0021-9673(01)01128-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

Calystegin A3 (RF-00004700-PAR)
Additional substance description:

calystegines A3 from root culture
Analytical method code:

Gaschromatography (GC) (F037A)
Result unit:

Microgram (G283A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.08
Internal remarks:

calystegines A3 from root culture

Composition #2: Scholl, Y., Asano, N., Drager, B. Automated multiple development thin layer chromatography for calystegines and their biosynthetic precursors . journal of chromatography a, 2001, vol. 928, no. 2, p. 217–224. DOI: 10.1016/S0021-9673(01)01128-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

Calystegine B2 (RF-00004703-PAR)
Additional substance description:

calystegines B2 and B1 from root culture
Analytical method code:

Gaschromatography (GC) (F037A)
Result unit:

Microgram (G283A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.51
Internal remarks:

calystegines B2 (0.51 microgram/spot ) and B1 (0.39) from root culture

Composition #3: Scholl, Y., Hoke, D., Drager, B. Calystegines in Calystegia sepium derive from the tropane alkaloid pathway . phytochemistry, 2001, vol. 58, no. 6, p. 883–889. DOI: 10.1016/S0031-9422(01)00362-4. [DOI link]

Language:

English (en)
Plant part:

Stem / stalk (as part-nature) (A067S)
Substance:

Calystegines (RF-00010841-PAR)
Result unit:

Gram/kilogram (G015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

5.7
Internal remarks:

Four-five days after germination, seedlings of Calystegia sepium showed the highest concentration of calystegines (5.7 mg/g dry mass).In the plants grown up to 19 cm in the climate chamber after 1 week, calistegins in roots were in the same range as in seedlings, while in the aerial parts calistegins were less concentrated

Composition #4: Scholl, Y., Hoke, D., Drager, B. Calystegines in Calystegia sepium derive from the tropane alkaloid pathway . phytochemistry, 2001, vol. 58, no. 6, p. 883–889. DOI: 10.1016/S0031-9422(01)00362-4. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Substance:

Calystegines (RF-00010841-PAR)
Result unit:

Gram/kilogram (G015A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.3
Internal remarks:

In 3 weeks , old plants young leaves were the tissue with the highest calystegin levels ( 2.3 mg/g dry mass). Calystegins A3 and B2 were the major components with a rather constant ratio (1:2-3) in all tissues , except flowers which contained similar concentrations of these two caystegins and of calystegin B1. Calystegins A5 and B1 were detected in most samples, but could not be quantified. Other calystegins cannot be excluded, but 2,7-dihydrixynortropane was not found in the aerial part.

Composition #5: Nakamura-Tsuruta, Sachiko., Uchiyama, Noboru., Peumans, Willy J., Van Damme, Els J. M., Totani, Kiichiro., Ito, Yukishige., Hirabayashi, Jun. Analysis of the sugar-binding specificity of mannose-binding-type Jacalin-related lectins by frontal affinity chromatography - an approach to functional classification . febs journal, 2008, vol. 275, no. 6, p. 1227–1239. DOI: 10.1111/j.1742-4658.2008.06282.x. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

Lectins (RF-00000439-ORG)
Additional substance description:

lectins ( Calystegia sepium agglutinin - Calsepa )
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lectins ( Calystegia sepium agglutinin - Calsepa )

Composition #6: EVANS, WC., SOMANABANDHU, AO. CUSCOHYGRINE - CONSTITUENT OF ROOTS OF SOME BRITISH CONVOLVULACEAE. phytochemistry, 1974, vol. 13, no. 2, p. 519–520. DOI: 10.1016/S0031-9422(00)91250-0. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

cuscohygrine (RF-00003758-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

IR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

cuscohygrine in roots

Composition #7: DRAGER, B. IDENTIFICATION AND QUANTIFICATION OF CALYSTEGINES, POLYHYDROXYL NORTROPANE ALKALOIDS . phytochemical analysis, 1995, vol. 6, no. 1, p. 31–37. DOI: 10.1002/pca.2800060105. [DOI link]

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Substance:

Calystegines (RF-00010841-PAR)
Additional substance description:

Tropane alkaloids
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Calystegines are a group of polyhydroxy nortropane alkaloids that have been isolated from Calystegia sepium