Composition #1: Bladh, Katarina Wedelsback., Olsson, Kerstin M., Yndgaard, Flemming. EVALUATION OF GLUCOSINOLATES IN NORDIC HORSERADISH (ARMORACIA RUSTICANA). botanica lithuanica, 2013, vol. 19, no. 1, p. 48–56. DOI: 10.2478/botlit-2013-0009.
[
DOI link]
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Plant part:
Roots and other underground parts (as part-nature) (A067M)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
maceration, ethanol-water extraction
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Substance:
sinigrin (RF-00004055-PAR)
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Analytical method code:
High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
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Result unit:
Millimole/kilogram (G070A)
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Result expression:
Dry matter (B002A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
10-45
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Internal remarks:
sinigrin in roots
Composition #2: Bladh, Katarina Wedelsback., Olsson, Kerstin M., Yndgaard, Flemming. EVALUATION OF GLUCOSINOLATES IN NORDIC HORSERADISH (ARMORACIA RUSTICANA). botanica lithuanica, 2013, vol. 19, no. 1, p. 48–56. DOI: 10.2478/botlit-2013-0009.
[
DOI link]
-
-
Plant part:
Roots and other underground parts (as part-nature) (A067M)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
maceration, ethanol-water extraction
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Substance:
gluconasturtiin (RF-00003843-PAR)
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Analytical method code:
High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
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Result unit:
Millimole/kilogram (G070A)
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Result expression:
Dry matter (B002A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
1.3-7.4
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Internal remarks:
gluconasturtiin in roots
Composition #3: Bladh, Katarina Wedelsback., Olsson, Kerstin M., Yndgaard, Flemming. EVALUATION OF GLUCOSINOLATES IN NORDIC HORSERADISH (ARMORACIA RUSTICANA). botanica lithuanica, 2013, vol. 19, no. 1, p. 48–56. DOI: 10.2478/botlit-2013-0009.
[
DOI link]
-
-
Plant part:
Roots and other underground parts (as part-nature) (A067M)
-
Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
maceration, ethanol-water extraction
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Substance:
glucobrassicin (RF-00003840-PAR)
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Analytical method code:
High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
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Result unit:
Millimole/kilogram (G070A)
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Result expression:
Dry matter (B002A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
0.1-0.26
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Internal remarks:
glucobrassicin in roots
Composition #4: Agneta, Rosa., Rivelli, Anna Rita., Ventrella, Emanuela., Lelario, Filomena., Sarli, Giulio., Bufo, Sabino Aurelio. Investigation of Glucosinolate Profile and Qualitative Aspects in Sprouts and Roots of Horseradish (Armoracia rusticana) Using LC-ESI Hybrid Linear Ion Trap with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Infrared Multiphoton Dissociation . journal of agricultural and food chemistry, 2012, vol. 60, no. 30, p. 7474–7482. DOI: 10.1021/jf301294h.
[
DOI link]
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Plant part:
Sprout (as part-nature) (A067R)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
methanol
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Substance:
Glucosinolates (RF-00000330-TOX)
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Additional substance description:
e.g. glucoiberin, sinigrin, gluconapin, glucocochlearin, glucoconringianin, glucosativin, glucoibarin, 5-hydroxyglucobrassicin, glucotropaeolin, glucobrassicin, gluconasturtiin, 4-methoxyglucobrassicin, glucoarabishirsutain
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Analytical method code:
LC-MS (F026A)
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Yes/No Qualitative:
Positive/Present (POS)
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Result type:
Qualitative Value (Binary) (BIN)
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Internal remarks:
Glucosinolates in sprouts
Composition #5: Agneta, Rosa., Rivelli, Anna Rita., Ventrella, Emanuela., Lelario, Filomena., Sarli, Giulio., Bufo, Sabino Aurelio. Investigation of Glucosinolate Profile and Qualitative Aspects in Sprouts and Roots of Horseradish (Armoracia rusticana) Using LC-ESI Hybrid Linear Ion Trap with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Infrared Multiphoton Dissociation . journal of agricultural and food chemistry, 2012, vol. 60, no. 30, p. 7474–7482. DOI: 10.1021/jf301294h.
[
DOI link]
-
-
Plant part:
Roots and other underground parts (as part-nature) (A067M)
-
Extraction/Preparation method:
Solvent extraction (A0BZR)
-
Additional extraction description:
methanol
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Substance:
Glucosinolates (RF-00000330-TOX)
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Additional substance description:
e.g. glucoiberin, sinigrin, glucosativin, glucoibarin, glucobrassicin, gluconasturtiin, 4-methoxyglucobrassicin, glucoarabishirsutain.
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Analytical method code:
LC-MS (F026A)
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Yes/No Qualitative:
Positive/Present (POS)
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Result type:
Qualitative Value (Binary) (BIN)
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Internal remarks:
Glucosinolates in roots
Endpoint study
#1: Hasumura, Mai., Imai, Toshio., Cho, Young-Man., Ueda, Makoto., Hirose, Masao., Nishikawa, Akiyoshi., Ogawa, Kumiko. Toxic effects of a horseradish extract and allyl isothiocyanate in the urinary bladder after 13-week administration in drinking water to F344 rats. journal of toxicological sciences, 2011, vol. 36, no. 6, p. 763–774. .
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Study relevance:
Human health
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Plant part:
Roots and other underground parts (as part-nature) (A067M)
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Preparation/Extraction method:
Solvent extraction (A0BZR)
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Additional plant/substance description:
steam distillation
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Type of toxicological test:
other (CHD008TT)
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Tested organism:
Rat (as animal) (A0CNV)
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Strain of test organism:
Fischer 344/DuCrj (CHD090ST)
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Route of exposure:
ORAL: DRINKING WATER (OECD2229PL)
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Unit of exposure duration:
Week (G205A)
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Number of organisms dosed:
100
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Endpoint measured:
Dose level (CHD014EP)
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Concentration unit:
Percent (G138A)
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Effect concentration:
0.025-0.1
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Critical effect:
NOT REPORTED (TOX17A)
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Target tissue:
urogenital: Urinary Bladder (TT051A)
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Effect description:
Increased dose-dependent urea nitrogen in serum and papillary nodular hyperplasia in bladder in higher doses and hyperplasia in bladder in all dosed groups.
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Effect or parameter linked to the endpoint:
histopathology non neoplastic (CHD025BE)
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Internal remarks:
Dose-dependent histopathological changes in urinary bladder
