EFSA Compendium of Botanicals

Draft - work in progress

Pseudotsuga menziesii (Mirb.) Franco

Family:: Pinaceae
Approval state:: Approved
Number of articles retrieved:: 8350
Number of processed references:: 3

Compositions

#1: Myrcene in twigs #2: Myrcene in needles #3: Tannins #4: Myrcene #5: Hydrolysable tannins

Endpoint Studies

Genotoxicity

#1: Ames test for Douglas tir condensate positive.

Composition #1: BUCHBAUER, G., JIROVETZ, L., WASICKY, M., NIKIFOROV, A. COMPARATIVE INVESTIGATION OF DOUGLAS-FIR HEADSPACE SAMPLES, ESSENTIAL OILS, AND EXTRACTS (NEEDLES AND TWIGS) USING GC-FID AND GC-FTIR-MS . journal of agricultural and food chemistry, 1994, vol. 42, no. 12, p. 2852–2854. DOI: 10.1021/jf00048a037. [DOI link]

Language:

English (en)
Plant part:

Stem / stalk (as part-nature) (A067S)
Additional extraction description:

Volatiles in the Headspace
Substance:

myrcene (RF-00003503-PAR)
Additional substance description:

in twigs
Analytical method code:

GC-FID (F040A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

4.11
Internal remarks:

Myrcene in twigs

Composition #2: BUCHBAUER, G., JIROVETZ, L., WASICKY, M., NIKIFOROV, A. COMPARATIVE INVESTIGATION OF DOUGLAS-FIR HEADSPACE SAMPLES, ESSENTIAL OILS, AND EXTRACTS (NEEDLES AND TWIGS) USING GC-FID AND GC-FTIR-MS . journal of agricultural and food chemistry, 1994, vol. 42, no. 12, p. 2852–2854. DOI: 10.1021/jf00048a037. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

petroleum ether
Substance:

myrcene (RF-00003503-PAR)
Additional substance description:

in needles
Analytical method code:

GC-FID (F040A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

3.18
Internal remarks:

Myrcene in needles

Composition #3: ZOU, JP., CATES, RG. FOLIAGE CONSTITUENTS OF DOUGLAS-FIR (PSEUDOTSUGA-MENZIESII (MIRB) FRANCO (PINACEAE)) - THEIR SEASONAL-VARIATION AND POTENTIAL ROLE IN DOUGLAS-FIR RESISTANCE AND SILVICULTURE MANAGEMENT . journal of chemical ecology, 1995, vol. 21, no. 4, p. 387–402. DOI: 10.1007/BF02036736. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetone 70%
Substance:

Tannins (RF-00004337-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

16.7-32
Internal remarks:

Tannins

Composition #4: ZOU, JP., CATES, RG. FOLIAGE CONSTITUENTS OF DOUGLAS-FIR (PSEUDOTSUGA-MENZIESII (MIRB) FRANCO (PINACEAE)) - THEIR SEASONAL-VARIATION AND POTENTIAL ROLE IN DOUGLAS-FIR RESISTANCE AND SILVICULTURE MANAGEMENT . journal of chemical ecology, 1995, vol. 21, no. 4, p. 387–402. DOI: 10.1007/BF02036736. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

CHCl3
Substance:

myrcene (RF-00003503-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/gram (G060A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.098-0.166
Internal remarks:

Myrcene

Composition #5: ZOU, JP., CATES, RG. FOLIAGE CONSTITUENTS OF DOUGLAS-FIR (PSEUDOTSUGA-MENZIESII (MIRB) FRANCO (PINACEAE)) - THEIR SEASONAL-VARIATION AND POTENTIAL ROLE IN DOUGLAS-FIR RESISTANCE AND SILVICULTURE MANAGEMENT . journal of chemical ecology, 1995, vol. 21, no. 4, p. 387–402. DOI: 10.1007/BF02036736. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetone 70%
Substance:

Ellagitannins (hydrolysable tannins) Ellagitannins (hydrolysable tannins) (RF-00004379-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

14
Internal remarks:

Hydrolysable tannins

Genotox #1: Singer, J.T., Jackson, J.A., Rice, R.W. Microbiological methods for the detection of mutagens and cytotoxic components in wood drying condensates from Douglas fir, red oak, southern yellow pine, yellow poplar and eastern white pine. journal of microbiological methods, 1995, vol. 22, no. 3, p. 229–242. DOI: 10.1016/0167-7012(95)00006-7. [DOI link]

Study category:

Mutagenicity
Type of toxicological test:

bacterial gene mutation assay (CHD027TT)
Method type:

in vitro
Tested organism:

Salmonella typhimurium (as organism) (A0CPZ)
Exogenous metabolic activation applied:

with
Control groups used:

yes
Endpoint measured:

gene mutation
Is genotoxic:

Positive
Remarks on genotox study:

wood drying condensates mutagenic after S9 activation with strains TA97a, TA100 ans TA102
Internal remarks:

Ames test for Douglas tir condensate positive.
Internal ID:

eid=2-s2.0-0029072793