EFSA Compendium of Botanicals

Draft - work in progress

Ononis spinosa L. // Ononis arvensis L. // Ononis spinosa subsp. hircina (Jacq.) Gams

Family:: Fabaceae (Leguminosae)
Approval state:: Approved
Number of articles retrieved:: 179
Number of processed references:: 10

Compositions

#1: Pterocarpan (trifolirhizin) #2: Carvone #3: Camphor #4: Estragole #5: Menthone and isomenthone #6: Naphthalene #7: Triterpenoid Saponin #8: Isoflavones #9: Isoflavones #10: Scopoletin #11: Lectins #12: Isoflavones #13: Formononetin #14: Biochanin A #15: Genistein

Endpoint Studies

Genotoxicity

Composition #1: Fujise, Yutaka., Toda, Takashi., Ito, Sho. Isolation of trifolirhizin from Ononis spinosa. chemical pharmaceutical bulletin, 1965, p. 93–95. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified
Substance:

Pterocarpans (RF-00010808-PAR)
Additional substance description:

trifolirhizin
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Pterocarpan (trifolirhizin)

Composition #2: Hilp, K., Kating, H., Schaden, G. Constituents of Ononis spinosa. 1. Essential oil of the radix ononidis. archiv der pharmazie (weinheim, germany), 1975, p. 429–433. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

steam distillation
Substance:

Carvone (RF-0538-001-PPP)
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Carvone

Composition #3: Hilp, K., Kating, H., Schaden, G. Constituents of Ononis spinosa. 1. Essential oil of the radix ononidis. archiv der pharmazie (weinheim, germany), 1975, p. 429–433. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

steam distillation
Substance:

Camphor (RF-00003986-PAR)
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Camphor

Composition #4: Hilp, K., Kating, H., Schaden, G. Constituents of Ononis spinosa. 1. Essential oil of the radix ononidis. archiv der pharmazie (weinheim, germany), 1975, p. 429–433. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

steam distillation
Substance:

Estragole (RF-00003806-PAR)
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Estragole

Composition #5: Hilp, K., Kating, H., Schaden, G. Constituents of Ononis spinosa. 1. Essential oil of the radix ononidis. archiv der pharmazie (weinheim, germany), 1975, p. 429–433. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Additional extraction description:

steam distillation
Substance:

menthone (RF-00011280-PAR)
Additional substance description:

Menthone and isomenthone
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Menthone and isomenthone

Composition #6: Hesse, Carmen., Hilp, Karin., Kating, Horst., Schaden, Gerhard. Constituents of Ononis spinosa L., part 2. Aromatic hydrocarbons in the essential oils of Radix and Herba Ononidis. archiv der pharmazie (weinheim, germany), 1977, p. 792–795. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Essential oil (as part-nature) (A166Q)
Substance:

Naphthalene derivatives (RF-00010833-PAR)
Analytical method code:

Gaschromatography (GC) (F037A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Naphthalene

Composition #7: Shaker, KH., Dockendorff, K., Bernhardt, M., Seifert, K. A new triterpenoid saponin from Ononis spinosa and two new flavonoid glycosides from Ononis vaginalis. ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES, 2004, vol. 59, no. 1, p. 124–128. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Ethanol and butanol
Substance:

Triterpene saponins Triterpene saponins (RF-00004338-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Triterpenoid Saponin

Composition #8: Klejdus, B., Vacek, J., Benesova, L., Kopecky, J., Lapcik, O., Kuban, V. Rapid-resolution HPLC with spectrometric detection for the determination and identification of isoflavones in soy preparations and plant extracts . analytical and bioanalytical chemistry, 2007, vol. 389, no. 7-8, p. 2277–2285. DOI: 10.1007/s00216-007-1606-3. [DOI link]

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

90% methanol
Substance:

isoflavones (RF-00004359-PAR)
Additional substance description:

pseudobaptigenin, glucoside, ononin, formononetin
Analytical method code:

HPLC-UV (F021A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isoflavones

Composition #9: Klejdus, B., Vacek, J., Lojkova, L., Benesova, L., Kuban, V. Ultrahigh-pressure liquid chromatography of isoflavones and phenolic acids on different stationary phases . journal of chromatography a, 2008, vol. 1195, no. 1-2, p. 52–59. DOI: 10.1016/j.chroma.2008.04.069. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

HCl
Substance:

isoflavones (RF-00004359-PAR)
Additional substance description:

Biochanin A and ononin
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isoflavones

Composition #10: Bajerova, Petra., Adam, Martin., Bajer, Tomas., Ventura, Karel. Comparison of various techniques for the extraction and determination of antioxidants in plants . journal of separation science, 2014, vol. 37, no. 7, p. 835–844. DOI: 10.1002/jssc.201301139. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol extraction. Other methods also used - supercritical fluid extraction, pressurized liquid extraction, ultrasonic extraction
Substance:

scopoletin (RF-00004048-PAR)
Additional substance description:

The range of concentrations reported is based on result of different extraction methods
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Microgram/100 gram (G046A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.3-44.0
Internal remarks:

Scopoletin

Composition #11: HOREJSI, V., KOCOUREK, J. STUDIES ON LECTINS .36. PROPERTIES OF SOME LECTINS PREPARED BY AFFINITY CHROMATOGRAPHY ON O-GLYCOSYL POLYACRYLAMIDE GELS. BIOCHIMICA ET BIOPHYSICA ACTA, 1978, vol. 538, no. 2, p. 299–315. DOI: 10.1016/0304-4165(78)90358-6. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified
Substance:

Lectins (RF-00000439-ORG)
Analytical method code:

Chromatographic tests (F015A)
Result unit:

Milligram/kilogram (G061A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

45
Internal remarks:

Lectins

Composition #12: KOSTER, J., STRACK, D., BARZ, W. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF ISOFLAVONES AND STRUCTURAL ELUCIDATION OF ISOFLAVONE 7-O-GLUCOSIDE 6''-MALONATES FROM CICER-ARIETINUM . planta medica, 1983, vol. 48, no. 3, p. 131–135. DOI: 10.1055/s-2007-969907. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

acetone
Substance:

isoflavones (RF-00004359-PAR)
Additional substance description:

biochanin A7-O-glc.; biochanin A7-O-glc.- 6"-malonate; genistein; formononetin; formononetin7-0-glc.; formononetin7-O-glc.- 6'-malonate
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isoflavones

Composition #13: PIETTA, P., CALATRONI, A., ZIO, C. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF FLAVONOIDS FROM ONONIS-SPINOSA L . journal of chromatography, 1983, vol. 280, no. 1, p. 172–175. DOI: 10.1016/S0021-9673(00)91555-3. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

50% ethanol
Substance:

formononetin (RF-00000291-NTR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

3.1-6.3
Internal remarks:

Formononetin

Composition #14: PIETTA, P., CALATRONI, A., ZIO, C. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF FLAVONOIDS FROM ONONIS-SPINOSA L . journal of chromatography, 1983, vol. 280, no. 1, p. 172–175. DOI: 10.1016/S0021-9673(00)91555-3. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

50% ethanol
Substance:

biochanin-A (RF-00004182-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.06-0.22
Internal remarks:

Biochanin A

Composition #15: PIETTA, P., CALATRONI, A., ZIO, C. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF FLAVONOIDS FROM ONONIS-SPINOSA L . journal of chromatography, 1983, vol. 280, no. 1, p. 172–175. DOI: 10.1016/S0021-9673(00)91555-3. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

50% ethanol
Substance:

genistein (RF-00000297-NTR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.3-6.6
Internal remarks:

Genistein