EFSA Compendium of Botanicals

Draft - work in progress

Gentiana cruciata L.

Family:: Gentianaceae
Approval state:: Approved
Number of articles retrieved:: 125
Number of processed references:: 2

Compositions

#1: tannins (aerial parts) #2: coumarins (aerial parts) #3: alkaloids (aerial parts) #4: gallic acid (aerial parts)

Endpoint Studies

Genotoxicity

#1: Positive Comet assay for extract from aerial parts

Composition #1: Borovik, T.S., Pribytkova, L.N. Devoted to comparative chemical and anatomical analysis of species Gentiana, which are growing on Western Siberia . voprosy biologicheskoi, meditsinskoi i farmatsevticheskoi khimii, 2014, no. 12, p. 33–34. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

Tannins (RF-00004337-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

UV detection
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

tannins (aerial parts)

Composition #2: Borovik, T.S., Pribytkova, L.N. Devoted to comparative chemical and anatomical analysis of species Gentiana, which are growing on Western Siberia . voprosy biologicheskoi, meditsinskoi i farmatsevticheskoi khimii, 2014, no. 12, p. 33–34. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

coumarins (RF-00004351-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

UV detection
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

coumarins (aerial parts)

Composition #3: Borovik, T.S., Pribytkova, L.N. Devoted to comparative chemical and anatomical analysis of species Gentiana, which are growing on Western Siberia . voprosy biologicheskoi, meditsinskoi i farmatsevticheskoi khimii, 2014, no. 12, p. 33–34. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

Alkaloids (RF-00004360-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

UV detection
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

alkaloids (aerial parts)

Composition #4: Borovik, T.S., Pribytkova, L.N. Devoted to comparative chemical and anatomical analysis of species Gentiana, which are growing on Western Siberia . voprosy biologicheskoi, meditsinskoi i farmatsevticheskoi khimii, 2014, no. 12, p. 33–34. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

gallic acid (RF-00003821-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

UV detection
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

gallic acid (aerial parts)

Genotox #1: Mihailovic, Vladimir., Misic, Danijela., Matic, Sanja., Mihailovic, Mirjana., Stanic, Snezana., Vrvic, Miroslav M., Katanic, Jelena., Mladenovic, Milan., Stankovic, Nevena., Boroja, Tatjana., Stankovic, Milan S. Comparative phytochemical analysis of Gentiana cruciata L. roots and aerial parts, and their biological activities. INDUSTRIAL CROPS AND PRODUCTS, 2015, vol. 73, p. 49–62. DOI: 10.1016/j.indcrop.2015.04.013. [DOI link]

Study category:

Genotoxicity
Type of toxicological test:

single cell gel/comet assay in mammalian cells for detection of DNA damage (CHD040TT)
Method type:

in vivo
Tested organism:

Rat (as animal) (A0CNV)
Strain of test organism:

Wistar (CHD013ST)
Sex:

Male
Exogenous metabolic activation applied:

not applicable
Route of exposure:

OTHER (OECD0PL)
Number of organisms dosed:

25
Control groups used:

yes
Endpoint measured:

DNA damage and/or repair
Is genotoxic:

Positive
Remarks on genotox study:

single dose extract i.p. positive in highest dose 400mg/kg bw
Internal remarks:

Positive Comet assay for extract from aerial parts
Internal ID:

WOS:000357839300006