EFSA Compendium of Botanicals

Draft - work in progress

Strawberries, wild (as plant) // Fragaria vesca L.

Family:: Rosaceae
Approval state:: Approved
Number of articles retrieved:: 1420
Number of processed references:: 3

Compositions

#1: gallic acid (leaf, quantified) #2: agrimoniin (leaf, quantified) #3: pedunculagin (leaf, quantified) #4: sanguiin H-6 #5: lambertianin C #6: agrimoniin

Endpoint Studies

Genotoxicity

#1: mutagenic in high dosages 5%, 10%, and 15% (wt/vol) Saccharomyces cervisiae reverse mutation test. also antimutagenic

Composition #1: Fecka, Izabela. Development of chromatographic methods for determination of agrimoniin and related polyphenols in pharmaceutical products. Journal of AOAC International, 2009, p. 410–418. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water methanol
Substance:

gallic acid (RF-00003821-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.11-0.16
Internal remarks:

gallic acid (leaf, quantified)

Composition #2: Fecka, Izabela. Development of chromatographic methods for determination of agrimoniin and related polyphenols in pharmaceutical products. Journal of AOAC International, 2009, p. 410–418. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water methanol
Substance:

Agrimoniin (RF-00010970-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.89-1.11
Internal remarks:

agrimoniin (leaf, quantified)

Composition #3: Fecka, Izabela. Development of chromatographic methods for determination of agrimoniin and related polyphenols in pharmaceutical products. Journal of AOAC International, 2009, p. 410–418. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

water methanol
Substance:

pedunculagin (RF-00011366-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.40-0.68
Internal remarks:

pedunculagin (leaf, quantified)

Composition #4: Vrhovsek, Urska., Guella, Graziano., Gasperotti, Mattia., Pojer, Elisa., Zancato, Mirella., Mattivi, Fulvio. Clarifying the Identity of the Main Ellagitannin in the Fruit of the Strawberry, Fragaria vesca and Fragaria ananassa Duch.. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, vol. 60, no. 10, p. 2507–2516. DOI: 10.1021/jf2052256. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Aqueous acetone
Substance:

sanguiin H-6 (RF-00004039-PAR)
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

HPLC, NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

sanguiin H-6

Composition #5: Vrhovsek, Urska., Guella, Graziano., Gasperotti, Mattia., Pojer, Elisa., Zancato, Mirella., Mattivi, Fulvio. Clarifying the Identity of the Main Ellagitannin in the Fruit of the Strawberry, Fragaria vesca and Fragaria ananassa Duch.. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, vol. 60, no. 10, p. 2507–2516. DOI: 10.1021/jf2052256. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Aqueous acetone
Substance:

lambertianin C (RF-00003893-PAR)
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

HPLC, NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

lambertianin C

Composition #6: Vrhovsek, Urska., Guella, Graziano., Gasperotti, Mattia., Pojer, Elisa., Zancato, Mirella., Mattivi, Fulvio. Clarifying the Identity of the Main Ellagitannin in the Fruit of the Strawberry, Fragaria vesca and Fragaria ananassa Duch.. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, vol. 60, no. 10, p. 2507–2516. DOI: 10.1021/jf2052256. [DOI link]

Language:

English (en)
Plant part:

Fruit unspecified (as part-nature) (A067D)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Aqueous acetone
Substance:

Agrimoniin (RF-00010970-PAR)
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

HPLC, NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

agrimoniin

Genotox #1: Spada, Patrcia D. S., Nunes de Souza, Gabrielle Gianna., Bortolini, Giovana Vera., Henriques, Joo A. P., Salvador, Mirian. Antioxidant, Mutagenic, and Antimutagenic Activity of Frozen Fruits. journal of medicinal food, 2008, p. 144–151. .

Study category:

Mutagenicity
Type of toxicological test:

bacterial reverse mutation assay (CHD028TT)
Method type:

in vitro
Guideline used:

No guideline followed
Tested organism:

Saccharomyces cerevisiae (as organism) (A0CPH)
Exogenous metabolic activation applied:

not applicable
Exposure duration for in vivo:

4.0
Unit of exposure duration:

Hour (G099A)
Control groups used:

yes
Endpoint measured:

gene mutation
Is genotoxic:

Positive
Remarks on genotox study:

frozen fruits mutagenic in high dosages 5%, 10%, and 15% (wt/vol); also showing antimutagenic activity
Internal remarks:

mutagenic in high dosages 5%, 10%, and 15% (wt/vol) Saccharomyces cervisiae reverse mutation test. also antimutagenic
Internal ID:

CAPLUS_2008:396710