EFSA Compendium of Botanicals

Draft - work in progress

Trifolium repens L.

Family:: Fabaceae
Approval state:: Approved
Number of articles retrieved:: 7607
Number of processed references:: 4

Compositions

#1: Lotaustralin (cyanogenic glycoside) #2: Cyanogenic glycosides (Linamarin and Lotaustralin) in seeds #3: Cyanogenic glycosides in leaves #4: Ononin (isoflavone) from dried blooming aerial parts #5: Daidzin (isoflavone) from dried blooming aerial parts #6: Formononetin (isoflavone) from dried blooming aerial parts #7: Genistein (isoflavone) from dried blooming aerial parts #8: Genistin (isoflavone) from dried blooming aerial parts

Endpoint Studies

Genotoxicity

Composition #1: Vickery, Margaret. Plant poisons: their occurrence, biochemistry and physiological properties. science progress (st. albans, united kingdom), 2010, p. 181–221. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified
Substance:

lotaustralin (RF-00003908-PAR)
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Lotaustralin (cyanogenic glycoside)

Composition #2: Butler, G.W., Butler, B.G. Biosynthesis of linamarin and lotaustralian in white clover. nature, 1960, vol. 187, no. 4739, p. 780–781. DOI: 10.1038/187780a0. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol 80%
Substance:

Cyanogenic glycosides (RF-00004375-PAR)
Additional substance description:

Linamarin and Lotaustralin
Analytical method code:

Chromatographic tests (F015A)
Result unit:

Gram/kilogram (G015A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.5
Internal remarks:

Cyanogenic glycosides (Linamarin and Lotaustralin) in seeds

Composition #3: Gleadow, Roslyn M., Edwards, Everard J., Evans, John R. Changes in Nutritional Value of Cyanogenic Trifolium repens Grown at Elevated Atmospheric CO2 . journal of chemical ecology, 2009, vol. 35, no. 4, p. 476–478. DOI: 10.1007/s10886-009-9617-5. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified
Substance:

Cyanogenic glycosides (RF-00004375-PAR)
Additional substance description:

mostly linamarin and a smaller proportion of lotaustralin
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Cyanogenic glycosides in leaves

Composition #4: Hanganu, Daniela., Vlase, Laurian., Olah, Neli. LC/MS ANALYSIS OF ISOFLAVONES FROM FABACEAE SPECIES EXTRACTS. farmacia, 2010, vol. 58, no. 2, p. 177–183. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH (50%)
Substance:

Ononin (RF-00011023-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/millilitre (G079A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

3190.3
Internal remarks:

Ononin (isoflavone) from dried blooming aerial parts

Composition #5: Hanganu, Daniela., Vlase, Laurian., Olah, Neli. LC/MS ANALYSIS OF ISOFLAVONES FROM FABACEAE SPECIES EXTRACTS. farmacia, 2010, vol. 58, no. 2, p. 177–183. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH (50%)
Substance:

daidzin (RF-00003763-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/millilitre (G079A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

741.1
Internal remarks:

Daidzin (isoflavone) from dried blooming aerial parts

Composition #6: Hanganu, Daniela., Vlase, Laurian., Olah, Neli. LC/MS ANALYSIS OF ISOFLAVONES FROM FABACEAE SPECIES EXTRACTS. farmacia, 2010, vol. 58, no. 2, p. 177–183. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH (50%)
Substance:

formononetin (RF-00000291-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/millilitre (G079A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1574.1
Internal remarks:

Formononetin (isoflavone) from dried blooming aerial parts

Composition #7: Hanganu, Daniela., Vlase, Laurian., Olah, Neli. LC/MS ANALYSIS OF ISOFLAVONES FROM FABACEAE SPECIES EXTRACTS. farmacia, 2010, vol. 58, no. 2, p. 177–183. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH (50%)
Substance:

genistein (RF-00000297-NTR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/millilitre (G079A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1082.8
Internal remarks:

Genistein (isoflavone) from dried blooming aerial parts

Composition #8: Hanganu, Daniela., Vlase, Laurian., Olah, Neli. LC/MS ANALYSIS OF ISOFLAVONES FROM FABACEAE SPECIES EXTRACTS. farmacia, 2010, vol. 58, no. 2, p. 177–183. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH (50%)
Substance:

genistin (RF-00003829-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Nanogram/millilitre (G079A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

4707.3
Internal remarks:

Genistin (isoflavone) from dried blooming aerial parts