Composition #1: Bennett, Richard N., Mellon, Fred A., Kroon, Paul A. Screening crucifer seeds as sources of specific intact glucosinolates using ion-pair high-performance liquid chromatography negative ion electrospray mass spectrometry. journal of agricultural and food chemistry, 2004, p. 428–438. .
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Plant part:
Seed (as part-nature) (A066P)
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Extraction/Preparation method:
Solvent extraction (A0BZR)
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Additional extraction description:
Methanol
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Substance:
Glucosinolates (RF-00000330-TOX)
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Additional substance description:
2-propenyl sinigrin
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Analytical method code:
HPLC with standard detection methods (F019A)
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Result unit:
Millimole/kilogram (G070A)
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Result expression:
Dry matter (B002A)
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Result type:
Numerical Value (VAL)
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Value (concentration, content):
50-75
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Internal remarks:
2-propenyl sinigrin
Endpoint study
#1: Smith R A., Crowe S P. Fanweed toxicosis in cattle: case history, analytical method, suggested treatment, and fanweed detoxification. veterinary and human toxicology, 1987, p. 155–156. .
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Study relevance:
Human health
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Substance:
Glucosinolates (RF-00000330-TOX)
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Plant part:
Aerial part of plants (as part-nature) (A166F)
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Preparation/Extraction method:
Raw, no heat treatment (A07HS)
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Additional plant/substance description:
feed was found to liberate 250 mg/100g of allylisothiocyanate
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Type of toxicological test:
short-term toxicity (CHD017TT)
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Tested organism:
Cattle (as animal) (A057E)
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Route of exposure:
ORAL: FEED (OECD2230PL)
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Unit of exposure duration:
Hour (G099A)
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Number of organisms dosed:
220
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Endpoint measured:
Dose not reported (CHD066EP)
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Critical effect:
Digestive (TOX18A)
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Target tissue:
digestive: Stomach (TT027A)
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Effect description:
distressed and colicy within 4 hr of feeding; Eight died over the next 5 days despite removal of the feed and symptomatic treatment; submucosal edema of the wall of the forestomachs. Four abortions.
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Effect or parameter linked to the endpoint:
mortality (CHD010BE)
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Internal remarks:
glucosinolates/isothiocyanate intoxication in cattle
