EFSA Compendium of Botanicals

Draft - work in progress

Smilax china L. // Coprosmanthus japonicus Kunth

Family:: Smilacaceae
Approval state:: Approved
Number of articles retrieved:: 512
Number of processed references:: 5

Compositions

#1: dioscin #2: Methylprotogracillin #3: Prosapogenin A #4: Gracillin #5: Methylprotodioscin #6: alkaloids #7: saponins #8: tannins #9: Phenylpropanoids (glucosides) #10: Gallic acid #11: Gentisic acid #12: Steroidal saponins

Endpoint Studies

Genotoxicity

Composition #1: Kim, Sung Whan., Chung, Kyu Charn., Son, Kun Ho., Kang, Sam Sik. Steroidal saponins from the rhizomes of Smilax china. Saengyak Hakhoechi, 1989, p. 76–82. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

dioscin (RF-00003782-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC, C and H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

dioscin

Composition #2: Kim, Sung Whan., Chung, Kyu Charn., Son, Kun Ho., Kang, Sam Sik. Steroidal saponins from the rhizomes of Smilax china. Saengyak Hakhoechi, 1989, p. 76–82. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

Methylprotogracillin (RF-00011257-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC, C and H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Methylprotogracillin

Composition #3: Kim, Sung Whan., Chung, Kyu Charn., Son, Kun Ho., Kang, Sam Sik. Steroidal saponins from the rhizomes of Smilax china. Saengyak Hakhoechi, 1989, p. 76–82. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

prosapogenin A (RF-00004008-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC, C and H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Prosapogenin A

Composition #4: Kim, Sung Whan., Chung, Kyu Charn., Son, Kun Ho., Kang, Sam Sik. Steroidal saponins from the rhizomes of Smilax china. Saengyak Hakhoechi, 1989, p. 76–82. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

Gracillin (RF-00011258-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC, C and H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Gracillin

Composition #5: Kim, Sung Whan., Chung, Kyu Charn., Son, Kun Ho., Kang, Sam Sik. Steroidal saponins from the rhizomes of Smilax china. Saengyak Hakhoechi, 1989, p. 76–82. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

methylprotodioscin (RF-00003934-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Analytical method - additional description:

TLC, C and H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Methylprotodioscin

Composition #6: Solomon Raju, B.G., Battu, G.R., Manju Latha, Y.B., Srinivas, K. Antihepatotoxic activity of smilax china roots on ccl 4 induced hepatic damage in rats. International Journal of Pharmacy and Pharmaceutical Sciences, 2012, vol. 4, no. 1, p. 494–496. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

Alkaloids (RF-00004360-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

alkaloids

Composition #7: Solomon Raju, B.G., Battu, G.R., Manju Latha, Y.B., Srinivas, K. Antihepatotoxic activity of smilax china roots on ccl 4 induced hepatic damage in rats. International Journal of Pharmacy and Pharmaceutical Sciences, 2012, vol. 4, no. 1, p. 494–496. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

Saponins (RF-00004333-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

saponins

Composition #8: Solomon Raju, B.G., Battu, G.R., Manju Latha, Y.B., Srinivas, K. Antihepatotoxic activity of smilax china roots on ccl 4 induced hepatic damage in rats. International Journal of Pharmacy and Pharmaceutical Sciences, 2012, vol. 4, no. 1, p. 494–496. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

Tannins (RF-00004337-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

tannins

Composition #9: Kuo, YH., Hsu, YW., Liaw, CC., Lee, JK., Huang, HC., Kuo, LMY. Cytotoxic phenylpropanoid glycosides from the stems of Smilax china. journal of natural products, 2005, vol. 68, no. 10, p. 1475–1478. DOI: 10.1021/np050109q. [DOI link]

Language:

English (en)
Plant part:

Stem / stalk (as part-nature) (A067S)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH 70%
Substance:

phenylpropanoids (RF-00004367-PAR)
Additional substance description:

Helonioside B; 2′,6′-Diacetyl-3,6-diferuloylsucrose; smilaside A to F; smiglaside E
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

HPLC fractionation, NMR, MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Phenylpropanoids (glucosides)

Composition #10: Yang, Chunhua., Tang, Qingfa., Liu, Jinghan., Zhang, Zunjian., Liu, Wenying. Preparative isolation and purification of phenolic acids from Smilax china by high-speed counter-current chromatography . separation and purification technology, 2008, vol. 61, no. 3, p. 474–478. DOI: 10.1016/j.seppur.2008.02.017. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH 70%
Substance:

gallic acid (RF-00003821-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

HSCCC, HPLC
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Gallic acid

Composition #11: Yang, Chunhua., Tang, Qingfa., Liu, Jinghan., Zhang, Zunjian., Liu, Wenying. Preparative isolation and purification of phenolic acids from Smilax china by high-speed counter-current chromatography . separation and purification technology, 2008, vol. 61, no. 3, p. 474–478. DOI: 10.1016/j.seppur.2008.02.017. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

EtOH 70%
Substance:

Salicylates (RF-00004331-PAR)
Additional substance description:

gentisic acid
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

HSCCC, HPLC
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Gentisic acid

Composition #12: SASHIDA, Y., KUBO, S., MIMAKI, Y., NIKAIDO, T., OHMOTO, T. STEROIDAL SAPONINS FROM SMILAX-RIPARIA AND S-CHINA. PHYTOCHEMISTRY, 1992, vol. 31, no. 7, p. 2439–2443. DOI: 10.1016/0031-9422(92)83295-A. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

steroidal saponins (RF-00010818-PAR)
Additional substance description:

isonarthogenin glucoside, dioscin
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

C and H NMR, MS, UV-Vis
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Steroidal saponins