EFSA Compendium of Botanicals

Draft - work in progress

Hyacinth bean (as plant) // Dolichos lablab L. // Lablab purpureus (L.) Sweet

Family:: Fabaceae
Approval state:: Approved
Number of articles retrieved:: 1158
Number of processed references:: 5

Compositions

#1: Trypsin inhibitors #2: Phytic acid #3: Haemagglutinin #4: Trypsin inhibitors #5: Trypsin inhibitor - unit per gram #6: Phenolic compounds (RF-00000010-ORG) #7: Proanthocyanidins (condensed tannins) (RF-00004370-PAR) #8: steroidal saponins

Endpoint Studies

#1: body weight decreased leading to mortality

Genotoxicity

Composition #1: Laurena, Antonio C., Revilleza, Ma. Jamela R., Mendona, Evelyn Mae T. Polyphenols, Phytate, Cyanogenic Glycosides, and Trypsin Inhibitor Activity of Several Philippine Indigenous Food Legumes. Journal of Food Composition and Analysis, 1994, vol. 7, no. 3, p. 194–202. DOI: 10.1006/jfca.1994.1019. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Additional extraction description:

inhibition activity expressed as unit per milligram
Substance:

Trypsin inhibitors Trypsin inhibitors (RF-00004339-PAR)
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

Trypsin inhibitor activity per mg sample
Result unit:

Unit (G005A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

14-27
Internal remarks:

Trypsin inhibitors

Composition #2: Laurena, Antonio C., Revilleza, Ma. Jamela R., Mendona, Evelyn Mae T. Polyphenols, Phytate, Cyanogenic Glycosides, and Trypsin Inhibitor Activity of Several Philippine Indigenous Food Legumes. Journal of Food Composition and Analysis, 1994, vol. 7, no. 3, p. 194–202. DOI: 10.1006/jfca.1994.1019. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Additional extraction description:

unspecified
Substance:

phytic acid (RF-00000363-NTR)
Analytical method code:

Colorimetry, Spectroscopy (Spectrometry) and Photometry (F008A)
Analytical method - additional description:

The value is milligram per gram P, not milligram per gram bean
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

8.5-10.1
Internal remarks:

Phytic acid

Composition #3: Ramamani, S., Subramanian, N., Parpia, H.A.B. Toxic and antigrowth effects of raw and processed field bean (Dolichos lablab) on albino rats. Journal of Biosciences, 1979, vol. 1, no. 2, p. 241–253. DOI: 10.1007/BF02706336. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Ethanol 80%
Substance:

Lectins (RF-00000439-ORG)
Additional substance description:

Haemagglutinin
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Haemagglutinin

Composition #4: Ramamani, S., Subramanian, N., Parpia, H.A.B. Toxic and antigrowth effects of raw and processed field bean (Dolichos lablab) on albino rats. Journal of Biosciences, 1979, vol. 1, no. 2, p. 241–253. DOI: 10.1007/BF02706336. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol 80%
Substance:

Trypsin inhibitors Trypsin inhibitors (RF-00004339-PAR)
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Trypsin inhibitors

Composition #5: Ignacimuthu, S., Janarthanan, S., Balachandran, B. Chemical basis of resistance in pulses to Callasobruchus maculatus (F.) (Coleoptera : Bruchidae) . journal of stored products research, 2000, vol. 36, no. 1, p. 89–99. DOI: 10.1016/S0022-474X(99)00031-4. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Tris-HCl.CaCl2
Substance:

Trypsin inhibitors Trypsin inhibitors (RF-00004339-PAR)
Additional substance description:

inhibition activity expressed as unit/gram
Analytical method code:

Gel electrophoresis (F076A)
Result unit:

Unit (G005A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

300
Internal remarks:

Trypsin inhibitor - unit per gram

Composition #6: Melaku, S., Peters, KJ., Tegegne, A. Supplementation of Menz ewes with dried leaves of Lablab purpureus or graded levels of Leucaena pallida 14203 and Sesbania sesban 1198: effects on feed intake, live weight gain and estrous cycle. ANIMAL FEED SCIENCE AND TECHNOLOGY, 2004, vol. 113, no. 1-4, p. 39–51. DOI: 10.1016/j.anifeedsci.2003.11.003. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

Phenolic compounds Phenolic compounds (RF-00000010-ORG)
Result unit:

Gram/kilogram (G015A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

10.1
Internal remarks:

Phenolic compounds (RF-00000010-ORG)

Composition #7: Melaku, S., Peters, KJ., Tegegne, A. Supplementation of Menz ewes with dried leaves of Lablab purpureus or graded levels of Leucaena pallida 14203 and Sesbania sesban 1198: effects on feed intake, live weight gain and estrous cycle. ANIMAL FEED SCIENCE AND TECHNOLOGY, 2004, vol. 113, no. 1-4, p. 39–51. DOI: 10.1016/j.anifeedsci.2003.11.003. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

Proanthocyanidins (condensed tannins) Proanthocyanidins (condensed tannins) (RF-00004370-PAR)
Result unit:

Gram/kilogram (G015A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

7.3
Internal remarks:

Proanthocyanidins (condensed tannins) (RF-00004370-PAR)

Composition #8: Ha, Tae Joung., Lee, Byong Won., Park, Ki Hun., Jeong, Seong Hun., Kim, Hyun-Tae., Ko, Jong-Min., Baek, In-Youl., Lee, Jin Hwan. Rapid characterisation and comparison of saponin profiles in the seeds of Korean Leguminous species using ultra performance liquid chromatography with photodiode array detector and electrospray ionisation/mass spectrometry (UPLC-PDA-ESI/MS) analysis. FOOD CHEMISTRY, 2014, vol. 146, p. 270–277. DOI: 10.1016/j.foodchem.2013.09.051. [DOI link]

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Additional extraction description:

methanol extraction
Substance:

steroidal saponins (RF-00010818-PAR)
Analytical method code:

Mass Spectroscopy and hyphenated methods without chromatography (F063A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

steroidal saponins

Endpoint study #1: Ramamani, S., Subramanian, N., Parpia, H.A.B. Toxic and antigrowth effects of raw and processed field bean (Dolichos lablab) on albino rats. Journal of Biosciences, 1979, vol. 1, no. 2, p. 241–253. DOI: 10.1007/BF02706336. [DOI link]

Study relevance:

Human health
Plant part:

Seed (as part-nature) (A066P)
Type of toxicological test:

other (CHD008TT)
Tested organism:

Rat (as animal) (A0CNV)
Sex:

Male/Female
Route of exposure:

ORAL: FEED (OECD2230PL)
Control groups used:

yes
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

SYSTEMIC (TOX01A)
Target tissue:

Digestive (TT002A)
Effect description:

body weight decreased leading to mortality
Effect or parameter linked to the endpoint:

mortality (CHD010BE)
Internal remarks:

body weight decreased leading to mortality