EFSA Compendium of Botanicals

Draft - work in progress

Hyoscyamus muticus L.

Family:: Solanaceae
Approval state:: Approved
Number of articles retrieved:: 414
Number of processed references:: 5

Compositions

#1: Atropine #2: Scopolamine #3: Atropine #4: Scopolamine #5: Tropane alkaloids (apoatropine, hyoscyamine, tropine) #6: Scopoletin #7: Erucylamide #8: Hygrine (pyrrolidine alkaloids) #9: Scopolamine #10: total alkaloids in flowers #11: total alkaloids in fruits #12: total alkaloids in leaves #13: total alkaloids in roots #14: total alkaloids in stems #15: Apoatropine #16: Hyoscyamine #17: Tropane alkaloids

Endpoint Studies

#1: Neurological collapse

Genotoxicity

Composition #1: Ashraf, Muhammad., Alam, Mahbub., Dara, M. Shahid Tauqeer. Nonaqueous determination of scopolamine and atropine in Hyoscyamus and Datura seeds. journal of pharmacy (lahore), 1985, p. 55–58. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified
Substance:

Atropine (RF-00000246-TOX)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Atropine

Composition #2: Ashraf, Muhammad., Alam, Mahbub., Dara, M. Shahid Tauqeer. Nonaqueous determination of scopolamine and atropine in Hyoscyamus and Datura seeds. journal of pharmacy (lahore), 1985, p. 55–58. .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

unspecified
Substance:

Scopolamine (RF-00000248-TOX)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Scopolamine

Composition #3: Mandal, S., Naqvi, A. A., Thakur, R. S. Simultaneous determination of atropine and scopolamine in plants by mixed-column high performance liquid chromatography. Phytochemical Analysis, 1991, p. 208–210. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol, dichlormethan, ammonium hydroxide
Substance:

Atropine (RF-00000246-TOX)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.32- 0.71
Internal remarks:

Atropine

Composition #4: Mandal, S., Naqvi, A. A., Thakur, R. S. Simultaneous determination of atropine and scopolamine in plants by mixed-column high performance liquid chromatography. Phytochemical Analysis, 1991, p. 208–210. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol, dichlormethan, ammonium hydroxide
Substance:

Scopolamine (RF-00000248-TOX)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.002- 0.04
Internal remarks:

Scopolamine

Composition #5: Dawidar, Abdel-Aziz M., Abdel-Mogib, Mamdouh., El-Naggar, Mahmoud E., Mostafa, Mohamed E. Acaricidal activity and chemical constituents of Hyoscyamus muticus against Tetranychus urticae Koch. Revista Latinoamericana de Quimica, 2009, p. 45–55. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

Tropane alkaloids (RF-00000245-TOX)
Additional substance description:

Apoatropine, hyoscyamine, tropine
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Tropane alkaloids (apoatropine, hyoscyamine, tropine)

Composition #6: Dawidar, Abdel-Aziz M., Abdel-Mogib, Mamdouh., El-Naggar, Mahmoud E., Mostafa, Mohamed E. Acaricidal activity and chemical constituents of Hyoscyamus muticus against Tetranychus urticae Koch. Revista Latinoamericana de Quimica, 2009, p. 45–55. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether, ether, methanol (1:1:1)
Substance:

scopoletin (RF-00004048-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Scopoletin

Composition #7: Dawidar, Abdel-Aziz M., Abdel-Mogib, Mamdouh., El-Naggar, Mahmoud E., Mostafa, Mohamed E. Acaricidal activity and chemical constituents of Hyoscyamus muticus against Tetranychus urticae Koch. Revista Latinoamericana de Quimica, 2009, p. 45–55. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

Erucic acid (RF-00000249-NTR)
Additional substance description:

Erucic acid derivative (Erucylamide)
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Erucylamide

Composition #8: El-Shazly, A., Tei, A., Witte, L., El-Domiaty, M., Wink, M. Tropane alkaloids of Hyoscyamus boveanus, H-desertorum, H-muticus and H-albus from Egypt . zeitschrift fur naturforschung c-a journal of biosciences, 1997, vol. 52, no. 11-12, p. 729–739. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkaloid extraction protocol
Substance:

Hygrine (RF-00011035-PAR)
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Hygrine (pyrrolidine alkaloids)

Composition #9: El-Shazly, A., Tei, A., Witte, L., El-Domiaty, M., Wink, M. Tropane alkaloids of Hyoscyamus boveanus, H-desertorum, H-muticus and H-albus from Egypt . zeitschrift fur naturforschung c-a journal of biosciences, 1997, vol. 52, no. 11-12, p. 729–739. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkaloid extraction protocol
Substance:

Scopolamine (RF-00000248-TOX)
Additional substance description:

expressed as percentage range of the total alkaloids found in the different plant parts
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.02-2.8
Internal remarks:

Scopolamine

Composition #10: El-Shazly, A., Tei, A., Witte, L., El-Domiaty, M., Wink, M. Tropane alkaloids of Hyoscyamus boveanus, H-desertorum, H-muticus and H-albus from Egypt . zeitschrift fur naturforschung c-a journal of biosciences, 1997, vol. 52, no. 11-12, p. 729–739. .

Language:

English (en)
Plant part:

Flower (as part-nature) (A067B)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkaloid extraction protocol
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

total alkaloids
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.25
Internal remarks:

total alkaloids in flowers

Composition #11: El-Shazly, A., Tei, A., Witte, L., El-Domiaty, M., Wink, M. Tropane alkaloids of Hyoscyamus boveanus, H-desertorum, H-muticus and H-albus from Egypt . zeitschrift fur naturforschung c-a journal of biosciences, 1997, vol. 52, no. 11-12, p. 729–739. .

Language:

English (en)
Plant part:

Fruit peel (as part-nature) (A067G)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkaloid extraction protocol
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

total alkaloids
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.02
Internal remarks:

total alkaloids in fruits

Composition #12: El-Shazly, A., Tei, A., Witte, L., El-Domiaty, M., Wink, M. Tropane alkaloids of Hyoscyamus boveanus, H-desertorum, H-muticus and H-albus from Egypt . zeitschrift fur naturforschung c-a journal of biosciences, 1997, vol. 52, no. 11-12, p. 729–739. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkaloid extraction protocol
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

total alkaloids
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.90
Internal remarks:

total alkaloids in leaves

Composition #13: El-Shazly, A., Tei, A., Witte, L., El-Domiaty, M., Wink, M. Tropane alkaloids of Hyoscyamus boveanus, H-desertorum, H-muticus and H-albus from Egypt . zeitschrift fur naturforschung c-a journal of biosciences, 1997, vol. 52, no. 11-12, p. 729–739. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkaloid extraction protocol
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

total alkaloids
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result expression:

Whole weight (B001A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.72
Internal remarks:

total alkaloids in roots

Composition #14: El-Shazly, A., Tei, A., Witte, L., El-Domiaty, M., Wink, M. Tropane alkaloids of Hyoscyamus boveanus, H-desertorum, H-muticus and H-albus from Egypt . zeitschrift fur naturforschung c-a journal of biosciences, 1997, vol. 52, no. 11-12, p. 729–739. .

Language:

English (en)
Plant part:

Stem / stalk (as part-nature) (A067S)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkaloid extraction protocol
Substance:

Alkaloids (RF-00004360-PAR)
Additional substance description:

total alkaloids
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

1.59
Internal remarks:

total alkaloids in stems

Composition #15: El-Shazly, A., Tei, A., Witte, L., El-Domiaty, M., Wink, M. Tropane alkaloids of Hyoscyamus boveanus, H-desertorum, H-muticus and H-albus from Egypt . zeitschrift fur naturforschung c-a journal of biosciences, 1997, vol. 52, no. 11-12, p. 729–739. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkaloid extraction protocol
Substance:

Apoatropine (RF-00004697-PAR)
Additional substance description:

expressed as percentage range of the total alkaloids found in the different plant parts
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2.55-11.65
Internal remarks:

Apoatropine

Composition #16: El-Shazly, A., Tei, A., Witte, L., El-Domiaty, M., Wink, M. Tropane alkaloids of Hyoscyamus boveanus, H-desertorum, H-muticus and H-albus from Egypt . zeitschrift fur naturforschung c-a journal of biosciences, 1997, vol. 52, no. 11-12, p. 729–739. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

alkaloid extraction protocol
Substance:

Hyoscyamine (RF-00000247-TOX)
Additional substance description:

expressed as percentage range of the total alkaloids found in the different plant parts
Analytical method code:

GC-MS (F046A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

80.52-96.43
Internal remarks:

Hyoscyamine

Composition #17: Oumerzouk, Jawad., Hssaini, Yahya., Boulahri, Tarik., Semlali, Adnane., El Jouehari, Abdelhafid., Bourazza, Ahmed. A massive poisoning by Hyoscyamus muticus, complicated by axonal sensorimotor polyneuropathy . presse medicale, 2013, vol. 42, no. 3, p. 354–355. DOI: 10.1016/j.lpm.2012.05.003. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

boiling water
Substance:

Tropane alkaloids (RF-00000245-TOX)
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Tropane alkaloids

Endpoint study #1: Oumerzouk, Jawad., Hssaini, Yahya., Boulahri, Tarik., Semlali, Adnane., El Jouehari, Abdelhafid., Bourazza, Ahmed. A massive poisoning by Hyoscyamus muticus, complicated by axonal sensorimotor polyneuropathy . presse medicale, 2013, vol. 42, no. 3, p. 354–355. DOI: 10.1016/j.lpm.2012.05.003. [DOI link]

Study relevance:

Human health
Substance:

Tropane alkaloids (RF-00000245-TOX)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

boiling water
Type of toxicological test:

case report (CHD060TT)
Tested organism:

Human (as organism) (A056J)
Sex:

Male
Route of exposure:

ORAL: DRINKING WATER (OECD2229PL)
Exposure duration:

1.0
Unit of exposure duration:

Meal (G109A)
Number of organisms dosed:

1
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

NEUROTOXICITY (TOX04A)
Target tissue:

neurologic: Central Nervous System (TT035A)
Effect description:

speech disorders, paresthesia, tingling sensations and in 15 hours, the patient fell into a coma
Effect or parameter linked to the endpoint:

neurology (CHD027BE)
Internal remarks:

Neurological collapse