EFSA Compendium of Botanicals

Draft - work in progress

Helleborus viridis L.

Family:: Ranunculaceae
Approval state:: Approved
Number of articles retrieved:: 45
Number of processed references:: 5

Compositions

#1: Helleborogenone (bufadienolides) #2: Corytuberine #3: Magnoflorine #4: hellebrin and derivatives #5: Steroidal furostan saponins

Endpoint Studies

#1: haemorrhage on the digestive tract #2: many petechiae, lungs congested, haemorrhage on the whole heart, abnormality of kidneys. Cardiac arrest in systole.

Genotoxicity

Composition #1: Wissner, W. 14-Hydroxy-3-oxo-1,4,20,22-bufatetraenolide, a new compound from underground organs of Helleborus species. Planta Medica, 1973, p. 201–210. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

Helleborogenone (RF-00011330-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Helleborogenone (bufadienolides)

Composition #2: Slavik, Jiri., Bochorakova, Jitka., Slavikova, Leonora. Occurrence of magnoflorine and corytuberine in some wild or cultivated plants of Czechoslovakia. Collection of Czechoslovak Chemical Communications, 1987, p. 804–811. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

Corytuberine (RF-00011231-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Value (concentration, content):

traces
Internal remarks:

Corytuberine

Composition #3: Slavik, Jiri., Bochorakova, Jitka., Slavikova, Leonora. Occurrence of magnoflorine and corytuberine in some wild or cultivated plants of Czechoslovakia. Collection of Czechoslovak Chemical Communications, 1987, p. 804–811. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Substance:

magnoflorine (RF-00003919-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Value (concentration, content):

traces
Internal remarks:

Magnoflorine

Composition #4: Colombo, M.L., Tome', F., Servettaz, O., Bugatti, C. Phytochemical evaluation of helleborus species growing in northern Italy. pharmaceutical biology, 1990, vol. 28, no. 3, p. 219–223. DOI: 10.3109/13880209009082817. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

pethroleum ether
Substance:

Hellebrin (RF-00011270-PAR)
Additional substance description:

hellebrin and derivatives
Analytical method code:

HPLC with standard detection methods (F019A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

hellebrin and derivatives

Composition #5: Stochmal, Anna., Perrone, Angela., Piacente, Sonia., Oleszek, Wieslaw. Saponins in aerial parts of Helleborus viridis L.. PHYTOCHEMISTRY LETTERS, 2010, vol. 3, no. 3, p. 129–132. DOI: 10.1016/j.phytol.2010.04.005. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

steroidal furostan saponins steroidal furostan saponins (RF-00004397-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Steroidal furostan saponins

Endpoint study #1: JOHNSON, CT., ROUTLEDG.JK. SUSPECTED HELLEBORUS VIRIDIS POISONING OF CATTLE. VETERINARY RECORD, 1971, vol. 89, no. 7, p. 202–&. .

Study relevance:

Human health
Plant part:

Aerial part of plants (as part-nature) (A166F)
Type of toxicological test:

case report (CHD060TT)
Limit test:

Not applicable
Tested organism:

Cattle (as animal) (A057E)
Sex:

No data
Route of exposure:

ORAL: UNSPECIFIED (OECD2234PL)
Number of organisms dosed:

5
Control groups used:

no
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

Digestive (TOX18A)
Target tissue:

Digestive (TT002A)
Effect description:

haemorrhage leading to delayed mortality
Effect or parameter linked to the endpoint:

mortality (CHD010BE)
Internal remarks:

haemorrhage on the digestive tract

Endpoint study #2: JOHNSON, CT., ROUTLEDG.JK. SUSPECTED HELLEBORUS VIRIDIS POISONING OF CATTLE. VETERINARY RECORD, 1971, vol. 89, no. 7, p. 202–&. .

Study relevance:

Human health
Plant part:

Aerial part of plants (as part-nature) (A166F)
Type of toxicological test:

case report (CHD060TT)
Limit test:

Not applicable
Tested organism:

Cattle (as animal) (A057E)
Sex:

No data
Route of exposure:

ORAL: UNSPECIFIED (OECD2234PL)
Number of organisms dosed:

5
Control groups used:

no
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

SYSTEMIC (TOX01A)
Target tissue:

cardiovascular / haematological: Heart (TT011A)
Effect description:

many petechiae, lungs congested, haemorrhage on the whole heart, abnormality of kidneys. Cardiac arrest in systole
Effect or parameter linked to the endpoint:

mortality (CHD010BE)
Internal remarks:

many petechiae, lungs congested, haemorrhage on the whole heart, abnormality of kidneys. Cardiac arrest in systole.