EFSA Compendium of Botanicals

Draft - work in progress

Helleborus purpurascens Waldst. & Kit.

Family:: Ranunculaceae
Approval state:: Approved
Number of articles retrieved:: 66
Number of processed references:: 4

Compositions

#1: Hellebrin #2: hellebrin #3: Steroidal saponin (dracoside)

Endpoint Studies

#1: Lethal effects in guinea pigs #2: Lethal effects in cats #3: 53.9% inhibition of alpha-chymotrypsin - water extract

Genotoxicity

Composition #1: Vorobev, N. E., Dzyuba, N. P. Quantitative determination of corelborin in roots of Helleborus purpurascens. farmatsevtichnii zhurnal (kiev), 1966, p. 37–40. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

Hellebrin (RF-00011270-PAR)
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.440-0.515
Internal remarks:

Hellebrin

Composition #2: Dirsch, V., Lacaille-Dubois, M.-A., Wagner, H. Dracoside, a new steroidal saponin from Helleborus purpurascens. Natural Product Letters, 1994, vol. 4, no. 1, p. 29–33. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

90% methanol
Substance:

Hellebrin (RF-00011270-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

hellebrin

Composition #3: Dirsch, V., Lacaille-Dubois, M.-A., Wagner, H. Dracoside, a new steroidal saponin from Helleborus purpurascens. Natural Product Letters, 1994, vol. 4, no. 1, p. 29–33. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

90% methanol
Substance:

Steroidal spirostane saponins Steroidal spirostane saponins (RF-00004321-PAR)
Additional substance description:

steroidal saponin (dracoside)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Steroidal saponin (dracoside)

Endpoint study #1: Buran, L., Babulova, A. The pharmacology of helleborin and deglucohelleborin, cardiac glycosides from Helleborus purpurascens. casopis lekaru ceskych, 1963, p. 570–574. .

Study relevance:

Human health
Substance:

cardiac glycosides (RF-00010823-PAR)
Additional plant/substance description:

Cardiac glycosides (helleborin and deglucohelleborin)
Type of toxicological test:

acute toxicity (CHD016TT)
Tested organism:

Guinea pig (as animal) (A0CTN)
Sex:

No data
Route of exposure:

ORAL: UNSPECIFIED (OECD2234PL)
Control groups used:

no data
Endpoint measured:

Lowest lethal dose (CHD025EP)
Concentration unit:

Milligram/kilogram (G061A)
Effect concentration:

0.516-0.214
Critical effect:

NOT REPORTED (TOX17A)
Effect description:

Minimum lethal dose
Effect or parameter linked to the endpoint:

not reported (CHD030BE)
Internal remarks:

Lethal effects in guinea pigs

Endpoint study #2: Buran, L., Babulova, A. The pharmacology of helleborin and deglucohelleborin, cardiac glycosides from Helleborus purpurascens. casopis lekaru ceskych, 1963, p. 570–574. .

Study relevance:

Human health
Substance:

cardiac glycosides (RF-00010823-PAR)
Additional plant/substance description:

Cardiac glycosides (helleborin and deglucohelleborin)
Type of toxicological test:

acute toxicity (CHD016TT)
Tested organism:

Cat (as animal) (A0CNM)
Sex:

No data
Route of exposure:

ORAL: UNSPECIFIED (OECD2234PL)
Control groups used:

no data
Endpoint measured:

Lowest lethal dose (CHD025EP)
Concentration unit:

Milligram/kilogram (G061A)
Effect concentration:

0.102-0.081
Critical effect:

NOT REPORTED (TOX17A)
Effect description:

Minimum lethal dose
Effect or parameter linked to the endpoint:

not reported (CHD030BE)
Internal remarks:

Lethal effects in cats

Endpoint study #3: Paun, Gabriela., Litescu, Simona Carmen., Neagu, Elena., Tache, Andreia., Radu, Gabriel Lucian. Evaluation of Geranium spp., Helleborus spp. and Hyssopus spp. polyphenolic extracts inhibitory activity against urease and alpha-chymotrypsin. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 2014, vol. 29, no. 1, p. 28–34. DOI: 10.3109/14756366.2012.749399. [DOI link]

Study relevance:

Human health
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

ultrasound-assisted extract of grounded plant material with cold distilled water as solvent
Type of toxicological test:

in vitro (CHD061TT)
Tested organism:

Human (as organism) (A056J)
Sex:

No data
Route of exposure:

OTHER (OECD0PL)
Control groups used:

no data
Endpoint measured:

Enzyme inhibition (CHD067EP)
Concentration unit:

Percent (G138A)
Effect concentration:

53.9
Critical effect:

Digestive (TOX18A)
Target tissue:

Digestive (TT002A)
Effect description:

53.9% inhibition of alpha-chymotrypsin
Effect or parameter linked to the endpoint:

enzyme inhibition (CHD032BE)
Internal remarks:

53.9% inhibition of alpha-chymotrypsin - water extract