EFSA Compendium of Botanicals

Draft - work in progress

Leptadenia reticulata (Retz.) Wight & Arn. // Gymnema aurantiacum Hook.f.

Family:: Apocyanaceae
Approval state:: Approved
Number of articles retrieved:: 151
Number of processed references:: 6

Compositions

#1: p-coumaric acid #2: 6-amino-3-hydroxy-4-(4'- methylphenyl)-2Hchromen-2-one (coumarin derivative) #3: Alkaloids #4: 3',5'-Dimethoxyacetophenone #5: Furan derivative #6: Saponins #7: Tannins #8: pregnanes derivatives (pregnant glycosides)

Endpoint Studies

#1: 100% of antiimplantation and abortion activity

Genotoxicity

Composition #1: Mammen, D., Daniel, M., Sane, R. T. Phytochemical investigation of water soluble phytoconstituents of Leptadenia reticulata (Retz.) Wight & Arn. international journal of research in pharmaceutical sciences, 2010, vol. 1, no. 4, p. 486–489. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

p-coumaric acid (RF-00010717-PAR)
Analytical method code:

UV/VIS spectroscopy (photometry) (F009A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

p-coumaric acid

Composition #2: Rajeswari, J., Rani, S. Anti-fertility efficacy of isolated compounds from Leptadenia reticulata and Lawsonia inermis. international journal of pharma and bio sciences, 2017, vol. 8, no. 1, p. 291–298. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

coumarins (RF-00004351-PAR)
Additional substance description:

6-amino-3-hydroxy-4-(4'- methylphenyl)-2Hchromen-2-one
Analytical method code:

Standard Chromatographic tests (paper- thin layer- and column chromatography) (F016A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

6-amino-3-hydroxy-4-(4'- methylphenyl)-2Hchromen-2-one (coumarin derivative)

Composition #3: Mammen, D., Daniel, M., Sane, R.T. Pharmacognostic and phytochemical studies on Leptadenia reticulata (Retz.) wight & arn. and Ichnocarpus frutescens R. Br. For identification of distinguishing biomarkers. pharmacognosy journal, 2011, vol. 2, no. 18, p. 7–12. DOI: 10.1016/S0975-3575(11)80019-2. [DOI link]

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol
Substance:

Alkaloids (RF-00004360-PAR)
Analytical method code:

Gelpermeation, High Performance Thin Layer Chromatography (HPTLC) (F017A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Alkaloids

Composition #4: Rajeswari, J., Rani, S. Gc-Ms analysis of whole plant of Leptadenia Reticulata. international journal of pharmtech research, 2014, vol. 6, no. 7, p. 2043–2050. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

1-phenylethanone (RF-00007224-PAR)
Additional substance description:

3',5'-Dimethoxyacetophenone
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

3',5'-Dimethoxyacetophenone

Composition #5: Rajeswari, J., Rani, S. Gc-Ms analysis of whole plant of Leptadenia Reticulata. international journal of pharmtech research, 2014, vol. 6, no. 7, p. 2043–2050. .

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

furan derivatives (RF-00004396-PAR)
Additional substance description:

2(4H)-Benzofuranone, 5,6,7,7atetrahydro-4,4,7a-trimethyl
Analytical method code:

GC-MS (F046A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Furan derivative

Composition #6: Rajeswari, J., Rani, S. Isolation and structural revelation of flavonoid constituents from Leptadenia reticulata Linn. der pharma chemica, 2016, vol. 8, no. 13, p. 70–76. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

Saponins (RF-00004333-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Saponins

Composition #7: Rajeswari, J., Rani, S. Isolation and structural revelation of flavonoid constituents from Leptadenia reticulata Linn. der pharma chemica, 2016, vol. 8, no. 13, p. 70–76. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

ethanol
Substance:

Tannins (RF-00004337-PAR)
Analytical method code:

Phytochemical screening (F604A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Tannins

Composition #8: SRIVASTAV, S., DEEPAK, D., KHARE, A. 3 NOVEL PREGNANE GLYCOSIDES FROM LEPTADENIA-RETICULATA WIGHT AND ARN. tetrahedron, 1994, vol. 50, no. 3, p. 789–798. DOI: 10.1016/S0040-4020(01)80794-8. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether
Substance:

pregnane derivatives (RF-00004313-PAR)
Additional substance description:

pregnane glycosides: Reticulin, Deniculatin and Leptaculatin
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

pregnanes derivatives (pregnant glycosides)

Endpoint study #1: Rajeswari, J., Rani, S. Anti-fertility efficacy of isolated compounds from Leptadenia reticulata and Lawsonia inermis. international journal of pharma and bio sciences, 2017, vol. 8, no. 1, p. 291–298. .

Study relevance:

Human health
Plant part:

Live plants (as part-nature) (A0BA0)
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

ethanol
Type of toxicological test:

reproduction toxicity (CHD009TT)
Tested organism:

Rat (as animal) (A0CNV)
Strain of test organism:

Wistar (CHD013ST)
Sex:

Female
Route of exposure:

ORAL: FEED (OECD2230PL)
Exposure duration:

10.0
Unit of exposure duration:

Day (G134A)
Number of organisms dosed:

6
Control groups used:

yes
Endpoint measured:

Dose level (CHD014EP)
Concentration unit:

Milligram/kilogram (G061A)
Effect concentration:

20
Critical effect:

REPRODUCTIVE (TOX05A)
Target tissue:

urogenital: Uterus (TT052A)
Effect description:

100% of antiimplantation and abortion activity
Effect or parameter linked to the endpoint:

reproduction (CHD014BE)
Internal remarks:

100% of antiimplantation and abortion activity