EFSA Compendium of Botanicals

Draft - work in progress

Glehnia littoralis F.Schmidt ex Miq.

Family:: Apiaceae
Approval state:: Approved
Number of articles retrieved:: 367
Number of processed references:: 5

Compositions

#1: Polyacetylene derivatives #2: Coumarins (scopoletin, impertorin,isoimperatorin, xanthotoxin,psoralen and bergapten) #3: Angelicin #4: Psoralen (aerial parts) #5: Psoralen (roots) #6: imperatorin (coumarin) in root #7: Isoimperatorin (coumarin) in root #8: Falcarindiol (polyethylene derivative) in root #9: Psoralen (coumarin) in root #10: Coumarins (glycosides of (S)-peucedanols, (S)-7-O-methylpeucedanols, marmesin and hydroxyimperatorin derivatives ) #11: Bergapten (coumarin) in aerial parts #12: Psoralen (coumarin) in aerial parts #13: Imperatorin (coumarin) in aerial parts #14: Isoimperatorin (coumarin) in root #15: Phellopterin (coumarin) in leaf #16: Xanthotoxin (or xanthotoxol) (coumarin) in aerial parts #17: Falcarindiol (polyacetylene derivative) aerial parts #18: Bergamottin (coumarin) in root #19: Bergapten (coumarin ) in root #20: Falcarindiol (polyacetylene derivative) in root #21: Imperatorin (coumarin) in root #22: Psoralen (coumarin) in root #23: Phellopterin (coumarin) in root #24: xanthotoxin (or xanthotoxol) (coumarin) in root

Endpoint Studies

#1: Prolonged sleeping time and analgesic effect

Genotoxicity

Composition #1: Okuyama, Emi., Hasegawa, Tetsuya., Matsushita, Takamitsu., Fujimoto, Haruhiro., Ishibashi, Masami., Yamazaki, Mikio., Hosokawa, Masakiyo., Hiraoka, Noboru., Anetai, Masaki., Masuda, Takahiro., Takasugi, Mitsuo. Analgesic components of Glehnia root (Glehnia littoralis). natural medicines, 1998, vol. 52, no. 6, p. 491–501. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

polyacetylene derivatives polyacetylene derivatives (RF-00004332-PAR)
Additional substance description:

panaxynol, falcarindiol and 8E-heptadeca-1,8-dien-4,6-diyn-3,10-diol
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Polyacetylene derivatives

Composition #2: Okuyama, Emi., Hasegawa, Tetsuya., Matsushita, Takamitsu., Fujimoto, Haruhiro., Ishibashi, Masami., Yamazaki, Mikio., Hosokawa, Masakiyo., Hiraoka, Noboru., Anetai, Masaki., Masuda, Takahiro., Takasugi, Mitsuo. Analgesic components of Glehnia root (Glehnia littoralis). natural medicines, 1998, vol. 52, no. 6, p. 491–501. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

coumarins (RF-00004351-PAR)
Additional substance description:

scopoletin, imperatorin, isoimperatorin, xanthotoxin, psoralen and bergapten
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Coumarins (scopoletin, impertorin,isoimperatorin, xanthotoxin,psoralen and bergapten)

Composition #3: Dong, Nguyen Thanh., Bae, KiHwan., Kim, Young Ho., Hwang, Gwi Seo., Heo, Ok Soon., Kim, Se Eun., Kang, Jong Seong. Quantitative determination of psoralen and angelicin from some medicinal herbs by high performance liquid chromatography. Archives of Pharmacal Research, 2003, p. 516–520. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

DCM
Substance:

angelicin (RF-00004146-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.016
Internal remarks:

Angelicin

Composition #4: Dong, Nguyen Thanh., Bae, KiHwan., Kim, Young Ho., Hwang, Gwi Seo., Heo, Ok Soon., Kim, Se Eun., Kang, Jong Seong. Quantitative determination of psoralen and angelicin from some medicinal herbs by high performance liquid chromatography. Archives of Pharmacal Research, 2003, p. 516–520. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

DCM
Substance:

psoralen (RF-00004015-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.024
Internal remarks:

Psoralen (aerial parts)

Composition #5: Dong, Nguyen Thanh., Bae, KiHwan., Kim, Young Ho., Hwang, Gwi Seo., Heo, Ok Soon., Kim, Se Eun., Kang, Jong Seong. Quantitative determination of psoralen and angelicin from some medicinal herbs by high performance liquid chromatography. Archives of Pharmacal Research, 2003, p. 516–520. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

DCM
Substance:

psoralen (RF-00004015-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/gram (G060A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.023
Internal remarks:

Psoralen (roots)

Composition #6: Li, Jun., Cheung, Anna W. H., Bi, Cathy W. C., Duan, Ran., Zheng, Ken Y. Z., Huang, Wen., Chen, Ji J., Dong, Tina T. X., Tsim, Karl W. K. Quality evaluation of Radix Glehniae (Glehnia littoralis) by HPLC-DAD: chromatographic fingerprinting and quantitative analysis of the herbs from different regions of China. Asian Journal of Traditional Medicines, 2010, p. 40–48. .

Language:

English (en)
Plant part:

Roots and other underground parts used as staple food (as part-nature) (A0F5F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

DCM
Substance:

imperatorin (RF-00003874-PAR)
Analytical method code:

HPLC-DAD (F587A)
Result unit:

Microgram/gram (G049A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.41-2.24
Internal remarks:

imperatorin (coumarin) in root

Composition #7: Li, Jun., Cheung, Anna W. H., Bi, Cathy W. C., Duan, Ran., Zheng, Ken Y. Z., Huang, Wen., Chen, Ji J., Dong, Tina T. X., Tsim, Karl W. K. Quality evaluation of Radix Glehniae (Glehnia littoralis) by HPLC-DAD: chromatographic fingerprinting and quantitative analysis of the herbs from different regions of China. Asian Journal of Traditional Medicines, 2010, p. 40–48. .

Language:

English (en)
Plant part:

Roots and other underground parts used as staple food (as part-nature) (A0F5F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

DCM
Substance:

isoimperatorin (RF-00003880-PAR)
Analytical method code:

HPLC-DAD (F587A)
Result unit:

Microgram/gram (G049A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.45-3.74
Internal remarks:

Isoimperatorin (coumarin) in root

Composition #8: Li, Jun., Cheung, Anna W. H., Bi, Cathy W. C., Duan, Ran., Zheng, Ken Y. Z., Huang, Wen., Chen, Ji J., Dong, Tina T. X., Tsim, Karl W. K. Quality evaluation of Radix Glehniae (Glehnia littoralis) by HPLC-DAD: chromatographic fingerprinting and quantitative analysis of the herbs from different regions of China. Asian Journal of Traditional Medicines, 2010, p. 40–48. .

Language:

English (en)
Plant part:

Roots and other underground parts used as staple food (as part-nature) (A0F5F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

DCM
Substance:

falcarindiol (RF-00003811-PAR)
Analytical method code:

HPLC-DAD (F587A)
Result unit:

Microgram/gram (G049A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

28.65-93.66
Internal remarks:

Falcarindiol (polyethylene derivative) in root

Composition #9: Li, Jun., Cheung, Anna W. H., Bi, Cathy W. C., Duan, Ran., Zheng, Ken Y. Z., Huang, Wen., Chen, Ji J., Dong, Tina T. X., Tsim, Karl W. K. Quality evaluation of Radix Glehniae (Glehnia littoralis) by HPLC-DAD: chromatographic fingerprinting and quantitative analysis of the herbs from different regions of China. Asian Journal of Traditional Medicines, 2010, p. 40–48. .

Language:

English (en)
Plant part:

Roots and other underground parts used as staple food (as part-nature) (A0F5F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

DCM
Substance:

psoralen (RF-00004015-PAR)
Analytical method code:

HPLC-DAD (F587A)
Result unit:

Microgram/gram (G049A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

>=3.77
Internal remarks:

Psoralen (coumarin) in root

Composition #10: Kitajima, J., Okamura, C., Ishikawa, T., Tanaka, Y. Coumarin glycosides of Glehnia littoralis root and rhizoma. CHEMICAL & PHARMACEUTICAL BULLETIN, 1998, vol. 46, no. 9, p. 1404–1407. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Methanol
Substance:

coumarins (RF-00004351-PAR)
Additional substance description:

glycosides of (S)-peucedanols, (S)-7-O-methylpeucedanols, marmesin and hydroxyimperatorin derivatives
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Coumarins (glycosides of (S)-peucedanols, (S)-7-O-methylpeucedanols, marmesin and hydroxyimperatorin derivatives )

Composition #11: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

Bergapten (RF-00003837-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

8-173
Internal remarks:

Bergapten (coumarin) in aerial parts

Composition #12: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

psoralen (RF-00004015-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=2
Internal remarks:

Psoralen (coumarin) in aerial parts

Composition #13: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

imperatorin (RF-00003874-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=10
Internal remarks:

Imperatorin (coumarin) in aerial parts

Composition #14: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

isoimperatorin (RF-00003880-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=1
Internal remarks:

Isoimperatorin (coumarin) in root

Composition #15: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Leaves (as part-nature) (A0EKT)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

Phellopterin (RF-00003560-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=5
Internal remarks:

Phellopterin (coumarin) in leaf

Composition #16: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

Xanthotoxol (RF-00011114-PAR)
Additional substance description:
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=21
Internal remarks:

Xanthotoxin (or xanthotoxol) (coumarin) in aerial parts

Composition #17: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

falcarindiol (RF-00003811-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

14-152
Internal remarks:

Falcarindiol (polyacetylene derivative) aerial parts

Composition #18: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

Bergamottin (RF-00003800-PAR)
Analytical method code:

HPLC with standard detection methods (F019A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=2.0
Internal remarks:

Bergamottin (coumarin) in root

Composition #19: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

Bergapten (RF-00003837-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

16-88
Internal remarks:

Bergapten (coumarin ) in root

Composition #20: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

falcarindiol (RF-00003811-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

124-250
Internal remarks:

Falcarindiol (polyacetylene derivative) in root

Composition #21: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

imperatorin (RF-00003874-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=1
Internal remarks:

Imperatorin (coumarin) in root

Composition #22: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

psoralen (RF-00004015-PAR)
Additional substance description:

psoralen derivative: 8-geranyloxypsolaren
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

2-58
Internal remarks:

Psoralen (coumarin) in root

Composition #23: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

Phellopterin (RF-00003560-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=1
Internal remarks:

Phellopterin (coumarin) in root

Composition #24: Hiraoka, N., Chang, JI., Bohm, LR., Bohm, BA. Furanocoumarin and polyacetylenic compound composition of wild Glehnia littoralis in North America. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2002, vol. 30, no. 4, p. 321–325. DOI: 10.1016/S0305-1978(01)00104-1. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Drying (dehydration) (A07KG)
Substance:

Xanthotoxol (RF-00011114-PAR)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Result unit:

Milligram/100 gram (G057A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

5-144
Internal remarks:

xanthotoxin (or xanthotoxol) (coumarin) in root

Endpoint study #1: Okuyama, Emi., Hasegawa, Tetsuya., Matsushita, Takamitsu., Fujimoto, Haruhiro., Ishibashi, Masami., Yamazaki, Mikio., Hosokawa, Masakiyo., Hiraoka, Noboru., Anetai, Masaki., Masuda, Takahiro., Takasugi, Mitsuo. Analgesic components of Glehnia root (Glehnia littoralis). natural medicines, 1998, vol. 52, no. 6, p. 491–501. .

Study relevance:

Human health
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

methanol and ethyl acetate
Type of toxicological test:

other (CHD008TT)
Tested organism:

House mouse (as animal) (A0CNT)
Strain of test organism:

ddy mouse (CHD069ST)
Sex:

Male
Route of exposure:

ORAL: UNSPECIFIED (OECD2234PL)
Endpoint measured:

Concentration level (CHD013EP)
Concentration unit:

Gram/kilogram (G015A)
Effect concentration:

1
Critical effect:

NEUROTOXICITY (TOX04A)
Target tissue:

Neurologic (TT004A)
Effect description:

prolonged pentobarbita induced sleeping time and analgesic effect
Effect or parameter linked to the endpoint:

neurology (CHD027BE)
Internal remarks:

Prolonged sleeping time and analgesic effect