EFSA Compendium of Botanicals

Draft - work in progress

Dioscorea collettii var. hypoglauca (Palib.) S.J.Pei & C.T.Ting // Dioscorea hypoglauca Palib.

Family:: Dioscoreaceae
Approval state:: Approved
Number of articles retrieved:: 142
Number of processed references:: 7

Compositions

#1: Saponins (total) #2: Steroidal saponins #3: hypoglaucin G #4: Steroidal saponins #5: Protoneodioscin #6: Gracillin and derivatives #7: Methyl protodioscin

Endpoint Studies

Genotoxicity

Composition #1: Li, Shu-qing., Xiao, Xiao., Wang, Kun-feng., Wu, Ling-fang., Zhang, Lan-zhen. Method for determination of total saponins in Dioscorea hypoglauca from different areas. zhongguo shiyan fangjixue zazhi, 2013, p. 118–120. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Substance:

Saponins (RF-00004333-PAR)
Additional substance description:

Total saponins (from different regions of China)
Substance used for equivalence:

Diosgenin (RF-00004094-PAR)
Analytical method code:

UV/VIS spectroscopy (photometry) (F009A)
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

0.84-2.85
Internal remarks:

Saponins (total)

Composition #2: Guo Long., Zeng Su-Ling., Zhang Yu., Li Ping., Liu E-Hu. Comparative analysis of steroidal saponins in four Dioscoreae herbs by high performance liquid chromatography coupled with mass spectrometry. journal of pharmaceutical and biomedical analysis, 2015, p. 91–98. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

65% ethanol
Substance:

steroidal saponins (RF-00010818-PAR)
Additional substance description:

Protodioscin (1.5-2.3%); Protogracillin (0.01-0.4%); Pseudoprotodioscin (0.02%); Pseudoprotogracillin (0.15%); Dioscin (0.15-0.93%) and Gracillin (0.07-0.23%)
Analytical method code:

High Performance Liquid Chromatography (HPLC)/Liquid Chromatography (LC) (F018A)
Analytical method - additional description:

HPLC-MS
Result unit:

Percent (G138A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

<=4
Internal remarks:

Steroidal saponins

Composition #3: Hu, K., Yao, XS., Dong, AJ., Kobayashi, H., Iwasaki, S., Jing, YK. A new pregnane glycoside from Dioscorea collettii var. hypoglauca. JOURNAL OF NATURAL PRODUCTS, 1999, vol. 62, no. 2, p. 299–301. DOI: 10.1021/np980101z. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

75% ethanol
Substance:

hypoglaucin G (RF-00011456-PAR)
Additional substance description:

Another pregnane derivative is also reported (pregna-5,16-dien-3 beta-ol-20-one 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)]-beta-D-glucopyranoside)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

hypoglaucin G

Composition #4: Hu, K., Dong, AJ., Yao, XS., Kobayashi, H., Iwasaki, S. Antineoplastic steroidal saponins from rhizomes of Dioscorea collettii var. hypoglauca. ADVANCES IN PLANT GLYCOSIDES, CHEMISTRY AND BIOLOGY, 1999, vol. 6, p. 220–229. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

75% ethanol, rhizoma
Substance:

steroidal saponins (RF-00010818-PAR)
Additional substance description:

dioscin, gracillin, protoneodioscin, protodioscin, protoneogracillin, protogracillin, methyl protoneodioscin, methyl protodioscin, methyl protoneogracillin, methyl protogracillin, hypoglaucin F, G and H
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Steroidal saponins

Composition #5: Hu, K., Yao, XS. The cytotoxicity of protoneodioscin (NSC-698789), a furostanol saponin from the rhizomes of Dioscorea collettii var. hypoglauca, against human cancer cells in vitro . phytomedicine, 2002, vol. 9, no. 6, p. 560–565. DOI: 10.1078/09447110260573218. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

dimethyl sulfoxide (DMSO)
Substance:

protodioscin (RF-00004009-PAR)
Additional substance description:

Protoneodioscin
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Protoneodioscin

Composition #6: Hu, K., Ya, XS. The cytotoxicity of methyl protoneogracillin (NSC-698793) and gracillin (NSC-698787), two steroidal saponins from the rhizomes of Dioscorea collettii var. hypoglauca, against human cancer cells in vitro . phytotherapy research, 2003, vol. 17, no. 6, p. 620–626. DOI: 10.1002/ptr.1211. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

steroidal saponins (RF-00010818-PAR)
Additional substance description:

Gracillin and derivatives
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Gracillin and derivatives

Composition #7: Hu, K., Yao, XS. The cytotoxicity of methyl protodioscin against human cancer cell lines in vitro . cancer investigation, 2003, vol. 21, no. 3, p. 389–393. DOI: 10.1081/CNV-120018230. [DOI link]

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Substance:

methylprotodioscin (RF-00003934-PAR)
Analytical method code:

Unspecified (F598A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Methyl protodioscin