EFSA Compendium of Botanicals

Draft - work in progress

Crotalaria sessiliflora L.

Family:: Fabaceae
Approval state:: Approved
Number of articles retrieved:: 69
Number of processed references:: 5

Compositions

#1: Integerrimine #2: Trichodesmine #3: Monocrotaline #4: Hydroquinone #5: Isoflavones #6: Isoflavones (7,2',4'-trihydroxyisoflavone-4'-O-beta-D-glucopyranoside) #7: Pyrrolizidine alkaloids

Endpoint Studies

#1: estrogenic activity, tumor promoting effect

Genotoxicity

Composition #1: Roeder, E., Liang, X. T., Kabus, K. J. Pyrrolizidine alkaloids from the seeds of Crotalaria sessiliflora. Planta Medica, 1992 . .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether , EtOH, HAc
Substance:

Integerrimine (RF-00000285-TOX)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Integerrimine

Composition #2: Roeder, E., Liang, X. T., Kabus, K. J. Pyrrolizidine alkaloids from the seeds of Crotalaria sessiliflora. Planta Medica, 1992 . .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether, ethanol HAc
Substance:

Trichodesmine (RF-00000306-TOX)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Trichodesmine

Composition #3: Roeder, E., Liang, X. T., Kabus, K. J. Pyrrolizidine alkaloids from the seeds of Crotalaria sessiliflora. Planta Medica, 1992 . .

Language:

English (en)
Plant part:

Seed (as part-nature) (A066P)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

Petroleum ether, EtOH HAc
Substance:

Monocrotaline (RF-00000238-TOX)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Monocrotaline

Composition #4: Mun'im, A., Negishi, O., Ozawa, T. Antioxidative compounds from Crotalaria sessiliflora. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2003, vol. 67, no. 2, p. 410–414. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

MeOH, EtOAc, n-ButOH
Substance:

Hydroquinone (RF-00007127-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

IR, FAB-MS
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Hydroquinone

Composition #5: Yoo, HS., Lee, JS., Kim, CY., Kim, J. Flavonoids of Crotalaria sessiliflora. ARCHIVES OF PHARMACAL RESEARCH, 2004, vol. 27, no. 5, p. 544–546. DOI: 10.1007/BF02980129. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Substance:

isoflavones (RF-00004359-PAR)
Additional substance description:

2',4',5,7-tetrahydroxyisoflavone and 2',4',7-trihydroxyisoflavone
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isoflavones

Composition #6: Mun'im, A., Isoda, H., Seki, M., Negishi, O., Ozawa, T. Estrogenic and acetylcholinesterase-enhancement activity of a new isoflavone, 7,2 ',4 '-trihydroxyisoflavone-4 '-O-beta-D-glucopyranoside from Crotalaria sessililflora. CYTOTECHNOLOGY, 2003, vol. 43, no. 1-3, p. 127–134. DOI: 10.1023/B:CYTO.0000039899.67160.18. [DOI link]

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

methanol extraction
Substance:

isoflavones (RF-00004359-PAR)
Additional substance description:

7,2',4'-trihydroxyisoflavone-4'-O-beta-D-glucopyranoside
Analytical method code:

HPLC with standard detection methods (F019A)
Analytical method - additional description:

NMR, IR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isoflavones (7,2',4'-trihydroxyisoflavone-4'-O-beta-D-glucopyranoside)

Composition #7: Lin, Ge., Li, Na., Li, Mi., Chan, Wood-Yee., Fu, Peter P., Xu, Hong-Xi. Potential biomarker for the assessment of hepatotoxicity induced by metabolic activation of pyrrolizidine alkaloids. DRUG METABOLISM REVIEWS, 2010, vol. 42, p. 207–207. .

Language:

English (en)
Plant part:

Unspecified (A07XD)
Substance:

Pyrrolizidine alkaloids (RF-00000230-TOX)
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Pyrrolizidine alkaloids

Endpoint study #1: Mun'im, A., Isoda, H., Seki, M., Negishi, O., Ozawa, T. Estrogenic and acetylcholinesterase-enhancement activity of a new isoflavone, 7,2 ',4 '-trihydroxyisoflavone-4 '-O-beta-D-glucopyranoside from Crotalaria sessililflora. CYTOTECHNOLOGY, 2003, vol. 43, no. 1-3, p. 127–134. DOI: 10.1023/B:CYTO.0000039899.67160.18. [DOI link]

Study relevance:

Human health
Substance:

isoflavones (RF-00004359-PAR)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Preparation/Extraction method:

Solvent extraction (A0BZR)
Additional plant/substance description:

7,2',4'-trihydroxyisoflavone-4'-O-beta-D-glucopyranoside
Type of toxicological test:

in vitro (CHD061TT)
Limit test:

Not applicable
Route of exposure:

OTHER (OECD0PL)
Control groups used:

yes
Endpoint measured:

Dose not reported (CHD066EP)
Critical effect:

ENDOCRINE (TOX12A)
Effect description:

estrogenic activity, tumor promoting effect
Effect or parameter linked to the endpoint:

Co-carcinogenic (CHD037BE)
Internal remarks:

estrogenic activity, tumor promoting effect