EFSA Compendium of Botanicals

Draft - work in progress

Berberis sibirica Pall.

Family:: Berberidaceae
Approval state:: Approved
Number of articles retrieved:: 12
Number of processed references:: 2

Compositions

#1: Pseudoprotopine (alkaloid) #2: Chelidonine (alkaloid) #3: Glaziovine (alkaloid) #4: Tetrahydropseudocoptisine (alkaloid) #5: Berberine (alkaloid) #6: Isothalidezine (alkaloid) #7: 8-oxoberberine (alkaloid) #8: 8-oxopalmatine (alkaloid) #9: Validivianine (alkaloid) #10: Armepavine (alkaloid) #11: Pakistanamine (alkaloid) #12: Pakistanine (alkaloid) #13: pronuciferine (alkaloid) #14: isocorydine (alkaloid) #15: Isothalidezine (alkaloid) #16: Isquinoline alkaloids #17: columbamine (alkaloid) #18: jatrorrhizine (alkaloid)

Endpoint Studies

Genotoxicity

Composition #1: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Pseudoprotopine (RF-00011423-PAR)
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Pseudoprotopine (alkaloid)

Composition #2: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

chelidonine (RF-00004205-PAR)
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Chelidonine (alkaloid)

Composition #3: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Glaziovine (RF-00011142-PAR)
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Glaziovine (alkaloid)

Composition #4: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Tetrahydropseudocoptisine (RF-00011427-PAR)
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Tetrahydropseudocoptisine (alkaloid)

Composition #5: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

berberine (RF-00003706-PAR)
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

separated by VLC on silica gel, then CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Berberine (alkaloid)

Composition #6: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

isothalidezine (RF-00011171-PAR)
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

separated by VLC on silica gel, then CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isothalidezine (alkaloid)

Composition #7: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Isoquinoline alkaloids (RF-00004391-PAR)
Additional substance description:

8-oxo-berberine
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

separated by VLC on silica gel, then CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

8-oxoberberine (alkaloid)

Composition #8: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Live plants (as part-nature) (A0BA0)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Oxypalmatine (RF-00011163-PAR)
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

separated by VLC on silica gel, then CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

8-oxopalmatine (alkaloid)

Composition #9: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Valdivianine (RF-00011150-PAR)
Additional substance description:
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

separated by VLC on silica gel, then CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Validivianine (alkaloid)

Composition #10: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Armepavine (RF-00011153-PAR)
Additional substance description:
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

separated by VLC on silica gel, then CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Armepavine (alkaloid)

Composition #11: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Pakistanamine (RF-00011143-PAR)
Additional substance description:
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

separated by VLC on silica gel, then CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Pakistanamine (alkaloid)

Composition #12: Istatkova, Ralitsa., Philipov, Stefan., Tuleva, Pepa., Amgalan, Solongo., Samdan, Javzan., Dangaa, Selenge. Alkaloids from Mongolian species Berberis sibirica Pall.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2007, vol. 60, no. 11, p. 1177–1182. .

Language:

English (en)
Plant part:

Roots and other underground parts (as part-nature) (A067M)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Pakistanine (RF-00011175-PAR)
Additional substance description:

Pakistanine and derivatives
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

separated by VLC on silica gel, then CC on neutra alumina, then PTLC, structures by comparison of its 1H NMR, EI MS, UV and IR data
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Pakistanine (alkaloid)

Composition #13: Istatkova, Ralitsa., Solongo, Amgalan., Javzan, Samdan., Selenge, Dangaa., Philipov, Stefan. ALKALOIDS FROM AERIAL PARTS OF BERBERIS SIBIRICA PALL.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2011, vol. 64, no. 2, p. 219–224. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

pronuciferine (RF-00011238-PAR)
Additional substance description:
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

UV, IR, EI MS and 1H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

pronuciferine (alkaloid)

Composition #14: Istatkova, Ralitsa., Solongo, Amgalan., Javzan, Samdan., Selenge, Dangaa., Philipov, Stefan. ALKALOIDS FROM AERIAL PARTS OF BERBERIS SIBIRICA PALL.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2011, vol. 64, no. 2, p. 219–224. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

isocorydine (RF-00003443-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

UV, IR, EI MS and 1H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

isocorydine (alkaloid)

Composition #15: Istatkova, Ralitsa., Solongo, Amgalan., Javzan, Samdan., Selenge, Dangaa., Philipov, Stefan. ALKALOIDS FROM AERIAL PARTS OF BERBERIS SIBIRICA PALL.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2011, vol. 64, no. 2, p. 219–224. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

isothalidezine (RF-00011171-PAR)
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

UV, IR, EI MS and 1H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isothalidezine (alkaloid)

Composition #16: Istatkova, Ralitsa., Solongo, Amgalan., Javzan, Samdan., Selenge, Dangaa., Philipov, Stefan. ALKALOIDS FROM AERIAL PARTS OF BERBERIS SIBIRICA PALL.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2011, vol. 64, no. 2, p. 219–224. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

Isoquinoline alkaloids (RF-00004391-PAR)
Additional substance description:

Dehydrocorypalline; 1-O-methylisotebaidine
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

UV, IR, EI MS and 1H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

Isquinoline alkaloids

Composition #17: Istatkova, Ralitsa., Solongo, Amgalan., Javzan, Samdan., Selenge, Dangaa., Philipov, Stefan. ALKALOIDS FROM AERIAL PARTS OF BERBERIS SIBIRICA PALL.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2011, vol. 64, no. 2, p. 219–224. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

columbamine (RF-00011147-PAR)
Additional substance description:
Analytical method code:

Nuclear Magnetic Resonance (NMR) (F061A)
Analytical method - additional description:

UV, IR, EI MS and 1H NMR
Yes/No Qualitative:

Positive/Present (POS)
Result type:

Qualitative Value (Binary) (BIN)
Internal remarks:

columbamine (alkaloid)

Composition #18: Istatkova, Ralitsa., Solongo, Amgalan., Javzan, Samdan., Selenge, Dangaa., Philipov, Stefan. ALKALOIDS FROM AERIAL PARTS OF BERBERIS SIBIRICA PALL.. COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, 2011, vol. 64, no. 2, p. 219–224. .

Language:

English (en)
Plant part:

Aerial part of plants (as part-nature) (A166F)
Extraction/Preparation method:

Solvent extraction (A0BZR)
Additional extraction description:

extracted with 95% EtOH, acidified with 5% HCl, extracted with n-hexane, alkalized with 25% NH4OH, extracted with CHCl3
Substance:

jatrorrhizine (RF-00003887-PAR)
Additional substance description:
Analytical method code:

Classification not possible (F001A)
Analytical method - additional description:

VLC on silica gel, CC on neutral alumina, then PTLC with Mph1, UV, IR, EI MS and 1H NMR
Result unit:

Milligram (G155A)
Result expression:

Dry matter (B002A)
Result type:

Numerical Value (VAL)
Value (concentration, content):

11.10
Internal remarks:

jatrorrhizine (alkaloid)